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- EMDB-1021: Structure of a viral DNA gatekeeper at 10 A resolution by cryo-el... -
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Basic information
Entry | Database: EMDB / ID: EMD-1021 | |||||||||
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Title | Structure of a viral DNA gatekeeper at 10 A resolution by cryo-electron microscopy. | |||||||||
![]() | Viral DNA gatekeeper. Centre of symmetry is 60.0,60.0,60.0 | |||||||||
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Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 10.0 Å | |||||||||
![]() | Orlova EV / Gowen B / Droege A / Stiege A / Weise F / Lurz R / van Heel M / Tavares P | |||||||||
![]() | ![]() Title: Structure of a viral DNA gatekeeper at 10 A resolution by cryo-electron microscopy. Authors: Elena V Orlova / Brent Gowen / Anja Dröge / Asita Stiege / Frank Weise / Rudi Lurz / Marin van Heel / Paulo Tavares / ![]() Abstract: In tailed bacteriophages and herpes viruses, the viral DNA is packaged through the portal protein channel. Channel closure is essential to prevent DNA release after packaging. Here we present the ...In tailed bacteriophages and herpes viruses, the viral DNA is packaged through the portal protein channel. Channel closure is essential to prevent DNA release after packaging. Here we present the connector structure from bacteriophage SPP1 using cryo-electron microscopy and single particle analysis. The multiprotein complex comprises the portal protein gp6 and the head completion proteins gp15 and gp16. Although we show that gp6 in the connector has a fold similar to that of the isolated portal protein, we observe conformational changes in the region of gp6 exposed to the DNA-packaging ATPase and to gp15. This reorganization does not cause closure of the channel. The connector channel traverses the full height of gp6 and gp15, but it is closed by gp16 at the bottom of the complex. Gp16 acts as a valve whose closure prevents DNA leakage, while its opening is required for DNA release upon interaction of the virus with its host. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 10.9 KB 10.9 KB | Display Display | ![]() |
Images | ![]() | 17.6 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Viral DNA gatekeeper. Centre of symmetry is 60.0,60.0,60.0 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Bacteriophage SPP1 connector
Entire | Name: Bacteriophage SPP1 connector |
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Components |
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-Supramolecule #1000: Bacteriophage SPP1 connector
Supramolecule | Name: Bacteriophage SPP1 connector / type: sample / ID: 1000 / Oligomeric state: cyclical dodecamer / Number unique components: 3 |
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Molecular weight | Experimental: 1.0 MDa / Theoretical: 960 KDa |
-Macromolecule #1: Bacteriophage SPP1 portal protein gp6
Macromolecule | Name: Bacteriophage SPP1 portal protein gp6 / type: protein_or_peptide / ID: 1 / Name.synonym: gp6 / Number of copies: 12 / Oligomeric state: cyclical dodecamer / Recombinant expression: No |
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Source (natural) | Organism: ![]() |
Molecular weight | Experimental: 57.3 KDa / Theoretical: 57.3 KDa |
-Macromolecule #2: gp15
Macromolecule | Name: gp15 / type: protein_or_peptide / ID: 2 / Name.synonym: head completion protein / Number of copies: 12 / Oligomeric state: cyclical dodecamer / Recombinant expression: No |
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Source (natural) | Organism: ![]() |
Molecular weight | Experimental: 11.6 KDa / Theoretical: 11.2 KDa |
-Macromolecule #3: head completion protein gp16
Macromolecule | Name: head completion protein gp16 / type: protein_or_peptide / ID: 3 / Name.synonym: gp16 / Number of copies: 12 / Oligomeric state: cyclical dodecamer / Recombinant expression: No |
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Source (natural) | Organism: ![]() |
Molecular weight | Experimental: 12.5 MDa / Theoretical: 12.1 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.9 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE / Instrument: HOMEMADE PLUNGER / Details: Vitrification instrument: general plunger / Method: blot for 2-3 second before plunging |
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Electron microscopy
Microscope | FEI/PHILIPS CM200FEG/ST |
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Temperature | Average: 90 K |
Date | Jan 1, 1999 |
Image recording | Category: FILM / Film or detector model: KODAK SO-163 FILM / Digitization - Scanner: PATCHWORK DENSITOMETER / Digitization - Sampling interval: 10.2 µm / Number real images: 21 / Average electron dose: 10 e/Å2 / Od range: 1.8 / Bits/pixel: 8 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 48600 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.1 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.7 µm / Nominal magnification: 50000 |
Sample stage | Specimen holder: side-entry / Specimen holder model: GATAN LIQUID NITROGEN |
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Image processing
CTF correction | Details: Each particle |
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Final reconstruction | Applied symmetry - Point group: C12 (12 fold cyclic) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 10.0 Å / Resolution method: OTHER / Software - Name: Imagic / Number images used: 4700 |
Final two d classification | Number classes: 260 |