+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-10013 | |||||||||
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Title | Cryo-ET on membrane protein | |||||||||
Map data | ||||||||||
Sample |
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Biological species | Bison bison (American bison) | |||||||||
Method | electron tomography / cryo EM | |||||||||
Authors | Zou XD / Zhao JJ / Xu HY / Carroni M | |||||||||
Funding support | Sweden, 2 items
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Citation | Journal: To Be Published Title: A simple pressure-assisted method for cryo-EM specimen preparation Authors: Zou XD / Zhao JJ / Xu HY / Carroni M | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_10013.map.gz | 67.4 MB | EMDB map data format | |
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Header (meta data) | emd-10013-v30.xml emd-10013.xml | 6.9 KB 6.9 KB | Display Display | EMDB header |
Images | emd_10013.png | 258.3 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-10013 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-10013 | HTTPS FTP |
-Validation report
Summary document | emd_10013_validation.pdf.gz | 156.9 KB | Display | EMDB validaton report |
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Full document | emd_10013_full_validation.pdf.gz | 156 KB | Display | |
Data in XML | emd_10013_validation.xml.gz | 4.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-10013 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-10013 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_10013.map.gz / Format: CCP4 / Size: 78.5 MB / Type: IMAGE STORED AS SIGNED BYTE | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 13.8 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Na+/H+ antiporter
Entire | Name: Na+/H+ antiporter |
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Components |
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-Supramolecule #1: Na+/H+ antiporter
Supramolecule | Name: Na+/H+ antiporter / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Bison bison (American bison) |
Recombinant expression | Organism: Saccharomyces cerevisiae (brewer's yeast) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | electron tomography |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
Sectioning | Other: NO SECTIONING |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.4 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Algorithm: BACK PROJECTION / Number images used: 50 |
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