|Entry||Database: EMDB / ID: EMD-0845|
|Title||Cryo-EM structure of African Swine Fever Virus capsid block 2|
|Sample||African swine fever virus:|
|Biological species||African swine fever virus|
|Method||single particle reconstruction / cryo EM / Resolution: 4.62 Å|
|Authors||Gao GF / Liu S|
|Funding support|| China, 1 items |
|Citation||Journal: Cell Host Microbe / Year: 2019|
Title: Cryo-EM Structure of the African Swine Fever Virus.
Authors: Sheng Liu / Yuzi Luo / Yajuan Wang / Shihua Li / Zhennan Zhao / Yuhai Bi / Junqing Sun / Ruchao Peng / Hao Song / Dongjie Zhu / Yuan Sun / Su Li / Li Zhang / Wei Wang / Yeping Sun / Jianxun Qi / Jinghua Yan / Yi Shi / Xinzheng Zhang / Peiyi Wang / Hua-Ji Qiu / George F Gao /
Abstract: African swine fever virus (ASFV) is a large double-stranded DNA virus with an icosahedral multilayered structure. ASFV causes a lethal swine hemorrhagic disease and is currently responsible for ...African swine fever virus (ASFV) is a large double-stranded DNA virus with an icosahedral multilayered structure. ASFV causes a lethal swine hemorrhagic disease and is currently responsible for widespread damage to the pork industry in Asia. Neither vaccines nor antivirals are available and the molecular characterization of the ASFV particle is outstanding. Here, we describe the cryogenic electron microscopy (cryo-EM) structure of the icosahedral capsid of ASFV at 4.6-Å. The ASFV particle consists of 8,280 copies of the major capsid protein p72, 60 copies of the penton protein, and at least 8,340 minor capsid proteins, of which there might be 3 different types. Like other nucleocytoplasmic large DNA viruses, the minor capsid proteins form a hexagonal network below the outer capsid shell, functioning as stabilizers by "gluing" neighboring capsomers together. Our findings provide a comprehensive molecular model of the ASFV capsid architecture that will contribute to the future development of countermeasures, including vaccines.
|Structure viewer||EM map: |
Downloads & links
|File||Download / File: emd_0845.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.41 Å|
|Symmetry||Space group: 1|
CCP4 map header:
-Entire African swine fever virus
|Entire||Name: African swine fever virus / Number of components: 1|
-Component #1: virus, African swine fever virus
|Virus||Name: African swine fever virus / Class: VIRION / Empty: No / Enveloped: Yes / Isolate: STRAIN|
|Species||Species: African swine fever virus|
|Specimen||Specimen state: Particle / Method: cryo EM|
|Sample solution||pH: 7.4|
|Vitrification||Cryogen name: ETHANE|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 1.25 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: GATAN K2 SUMMIT (4k x 4k)|
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