SASDH52: Ribonuclease A (Type III-A) in a solution environment at pH 7.5

Basic information

• Entry: Database: SASBDB / ID: SASDH52

Sample

Ribonuclease A (Type III-A) in a solution environment at pH 7.5

• Ribonuclease pancreatic (protein), RNase-A, Bos taurus

Function / homology

Function:

pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / defense response to Gram-positive bacterium / lyase activity / extracellular region

Domain/homology:

Pancreatic ribonuclease / Ribonuclease A, active site / Ribonuclease A-domain / Ribonuclease A-like domain superfamily / Pancreatic ribonuclease / Pancreatic ribonuclease family signature. / Pancreatic ribonuclease

Component:

Ribonuclease pancreatic

• Biological species: Bos taurus (domestic cattle)

Contact author

• Chandra Sekar (Pondicherry University)

Models

• Model #3898: ; Type: dummy / Software: (dammif (r9988)) / Radius of dummy atoms: 1.80 A / Symmetry: P1 / Chi-square value: 1.265 / P-value: 0.073676
• Model #3899: ; Type: dummy / Radius of dummy atoms: 1.80 A / Symmetry: P1 / Chi-square value: 1.265 / P-value: 0.073676
• Model #3900: ; Type: dummy / Radius of dummy atoms: 1.80 A / Symmetry: P1 / Chi-square value: 1.262 / P-value: 0.142303
• Model #3901: ; Type: dummy / Software: (dammif (r9988)) / Radius of dummy atoms: 1.80 A / Symmetry: P1 / Chi-square value: 1.262 / P-value: 0.142303
• Model #3902: ; Type: dummy / Software: (DAMFILT 5.0 (r9988)) / Radius of dummy atoms: 2.00 A / Symmetry: P1 / Chi-square value: 1.268 / P-value: 0.073676

Sample

• Sample: Name: Ribonuclease A (Type III-A) in a solution environment at pH 7.5; Specimen concentration: 2.50-10.00
• Buffer: Name: 50 mM Tris-HCL, 150 mM NaCl / pH: 7.5 ; Comment: PDI was incubated with 0.5mM TCEP to reduce the Active sites one hour before SAXS data collection

Entity #1950

Name: RNase-A / Type: protein / Description: Ribonuclease pancreatic / Formula weight: 13.69 / Num. of mol.: 1 / Source: Bos taurus / References: UniProt: P61823
Sequence:

KETAAAKFER QHMDSSTSAA SSSNYCNQMM KSRNLTKDRC KPVNTFVHES LADVQAVCSQ KNVACKNGQT NCYQSYSTMS ITDCRETGSS KYPNCAYKTT QANKHIIVAC EGNPYVPVHF DASV

Experimental information

• Beam: Instrument name: Advanced Photon Source (APS), Argonne National Laboratory BioCAT 18ID; City: Lemont, IL / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 3.485 mm
• Detector: Name: Pilatus3 X 1M / Pixsize x: 0.172 mm

Scan

Title: Ribonuclease A (Type III-A) in a solution environment at pH 7.5
Measurement date: Nov 4, 2015 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 8 / Unit: 1/A /

	Min	Max
Q	0.0219	0.212

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 637 /

	Min	Max
Q	0.0218908	0.212003
P(R) point	1	637
R	0	72.17

Result

Type of curve: merged
Comments: Ribonuclease A (Type III-A) was purchased from SIGMA-ALDRICH in the form of lyophilized powder, dissolved with suspension medium and used directly without any further purification.

	Experimental	Standard	Porod
MW	27.38 kDa	16.926 kDa	12.5 kDa
Volume	-	-	20 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	7.684	7.61	0.035
Radius of gyration, Rg	1.94 nm	1.86 nm	0.02

	Min	Max
D	-	7.22
Guinier point	1	162