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- SASDH42: Bovine Pancreatic Trypsin Inhibitor (BPTI) in solution environmen... -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDH42
SampleBovine Pancreatic Trypsin Inhibitor (BPTI) in solution environment at pH 7.5
  • Pancreatic trypsin inhibitor (protein), BPTI, Bos taurus
Function / homology
Function and homology information


trypsinogen activation / negative regulation of serine-type endopeptidase activity / sulfate binding / potassium channel inhibitor activity / negative regulation of platelet aggregation / zymogen binding / molecular function inhibitor activity / negative regulation of thrombin-activated receptor signaling pathway / serine protease inhibitor complex / serine-type endopeptidase inhibitor activity ...trypsinogen activation / negative regulation of serine-type endopeptidase activity / sulfate binding / potassium channel inhibitor activity / negative regulation of platelet aggregation / zymogen binding / molecular function inhibitor activity / negative regulation of thrombin-activated receptor signaling pathway / serine protease inhibitor complex / serine-type endopeptidase inhibitor activity / protease binding / calcium ion binding / extracellular space
Similarity search - Function
: / Proteinase inhibitor I2, Kunitz, conserved site / Pancreatic trypsin inhibitor (Kunitz) family signature. / BPTI/Kunitz family of serine protease inhibitors. / Pancreatic trypsin inhibitor Kunitz domain / Kunitz/Bovine pancreatic trypsin inhibitor domain / Pancreatic trypsin inhibitor (Kunitz) family profile. / Pancreatic trypsin inhibitor Kunitz domain superfamily
Similarity search - Domain/homology
Pancreatic trypsin inhibitor
Similarity search - Component
Biological speciesBos taurus (domestic cattle)
Contact author
  • Chandra Sekar (Pondicherry University)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3893
Type: dummy / Software: (dammif (r9988)) / Radius of dummy atoms: 1.00 A / Symmetry: P1 / Chi-square value: 1.318 / P-value: 0.000004
Model #3894
Type: dummy / Radius of dummy atoms: 1.00 A / Symmetry: P1 / Chi-square value: 1.318 / P-value: 0.000004
Model #3895
Type: dummy / Software: (dammif (r9988)) / Radius of dummy atoms: 1.00 A / Symmetry: P1 / Chi-square value: 1.320 / P-value: 0.000004
Model #3896
Type: dummy / Radius of dummy atoms: 0.97 A / Symmetry: P1 / Chi-square value: 1.320 / P-value: 0.000004
Model #3897
Type: dummy / Software: (DAMFILT 5.0 (r9988)) / Radius of dummy atoms: 1.25 A / Symmetry: P1 / Chi-square value: 1.318 / P-value: 0.000004

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Sample

SampleName: Bovine Pancreatic Trypsin Inhibitor (BPTI) in solution environment at pH 7.5
Specimen concentration: 2.50-10.00
BufferName: 50 mM Tris-HCL, 150 mM NaCl / pH: 7.5
Comment: PDI was incubated with 0.5mM TCEP to reduce the Active sites one hour before SAXS data collection
Entity #1949Name: BPTI / Type: protein / Description: Pancreatic trypsin inhibitor / Formula weight: 6.517 / Num. of mol.: 2 / Source: Bos taurus / References: UniProt: P00974
Sequence:
RPDFCLEPPY TGPCKARIIR YFYNAKAGLC QTFVYGGCRA KRNNFKSAED CMRTCGGA

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Experimental information

BeamInstrument name: Advanced Photon Source (APS), Argonne National Laboratory BioCAT 18ID
City: Lemont, IL / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 3.485 mm
DetectorName: Pilatus3 X 1M / Pixsize x: 0.172 mm
Scan
Title: Bovine Pancreatic Trypsin Inhibitor (BPTI) in solution environment at pH 7.5
Measurement date: Nov 4, 2015 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 8 / Unit: 1/A /
MinMax
Q0.0258 0.392
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1208 /
MinMax
Q0.0311573 0.391952
P(R) point1 1208
R0 45.65
Result
Type of curve: merged
Comments: Bovine Pancreatic Trypsin Inhibitor (BPTI) was purchased from SIGMA-ALDRICH in the form of lyophilized powder, dissolved with suspension medium and used directly without any further ...Comments: Bovine Pancreatic Trypsin Inhibitor (BPTI) was purchased from SIGMA-ALDRICH in the form of lyophilized powder, dissolved with suspension medium and used directly without any further purification. CAUTION! Sample likely aggregated.
ExperimentalStandardPorod
MW13.034 kDa22.36 kDa3.6 kDa
Volume--5.8 nm3

P(R)GuinierGuinier error
Forward scattering, I08.437 8.55 0.032
Radius of gyration, Rg1.42 nm1.42 nm0.05

MinMax
D-4.56
Guinier point19 218

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