SASDH42: Bovine Pancreatic Trypsin Inhibitor (BPTI) in solution environmen...

Basic information

• Entry: Database: SASBDB / ID: SASDH42

Sample

Bovine Pancreatic Trypsin Inhibitor (BPTI) in solution environment at pH 7.5

• Pancreatic trypsin inhibitor (protein), BPTIAprotinin, Bos taurus

Function / homology

Function:

trypsinogen activation / negative regulation of serine-type endopeptidase activity / sulfate binding / potassium channel inhibitor activity / negative regulation of platelet aggregation / zymogen binding / molecular function inhibitor activity / negative regulation of thrombin-activated receptor signaling pathway / serine protease inhibitor complex / serine-type endopeptidase inhibitor activity / protease binding / calcium ion binding / extracellular space

Domain/homology:

Proteinase inhibitor I2, Kunitz, conserved site / Pancreatic trypsin inhibitor (Kunitz) family signature. / BPTI/Kunitz family of serine protease inhibitors. / Pancreatic trypsin inhibitor Kunitz domain / Kunitz/Bovine pancreatic trypsin inhibitor domain / Pancreatic trypsin inhibitor (Kunitz) family profile. / Pancreatic trypsin inhibitor Kunitz domain superfamily

Component:

Pancreatic trypsin inhibitor

• Biological species: Bos taurus (cattle)

Contact author

• Chandra Sekar (Pondicherry University)

Models

• Model #3893: ; Type: dummy / Software: (dammif (r9988)) / Radius of dummy atoms: 1.00 A / Symmetry: P1 / Chi-square value: 1.318 / P-value: 0.000004
• Model #3894: ; Type: dummy / Radius of dummy atoms: 1.00 A / Symmetry: P1 / Chi-square value: 1.318 / P-value: 0.000004
• Model #3895: ; Type: dummy / Software: (dammif (r9988)) / Radius of dummy atoms: 1.00 A / Symmetry: P1 / Chi-square value: 1.320 / P-value: 0.000004
• Model #3896: ; Type: dummy / Radius of dummy atoms: 0.97 A / Symmetry: P1 / Chi-square value: 1.320 / P-value: 0.000004
• Model #3897: ; Type: dummy / Software: (DAMFILT 5.0 (r9988)) / Radius of dummy atoms: 1.25 A / Symmetry: P1 / Chi-square value: 1.318 / P-value: 0.000004

Sample

• Sample: Name: Bovine Pancreatic Trypsin Inhibitor (BPTI) in solution environment at pH 7.5; Specimen concentration: 2.50-10.00
• Buffer: Name: 50 mM Tris-HCL, 150 mM NaCl / pH: 7.5 ; Comment: PDI was incubated with 0.5mM TCEP to reduce the Active sites one hour before SAXS data collection

Entity #1949

Name: BPTIAprotinin / Type: protein / Description: Pancreatic trypsin inhibitor / Formula weight: 6.517 / Num. of mol.: 2 / Source: Bos taurus / References: UniProt: P00974
Sequence:

RPDFCLEPPY TGPCKARIIR YFYNAKAGLC QTFVYGGCRA KRNNFKSAED CMRTCGGA

Experimental information

• Beam: Instrument name: Advanced Photon Source (APS), Argonne National Laboratory BioCAT 18ID; City: Lemont, IL / 国: USA / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 3.485 mm
• Detector: Name: Pilatus3 X 1M / Pixsize x: 0.172 mm

Scan

Title: Bovine Pancreatic Trypsin Inhibitor (BPTI) in solution environment at pH 7.5
Measurement date: Nov 4, 2015 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 8 / Unit: 1/A /

	Min	Max
Q	0.0258	0.392

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 1208 /

	Min	Max
Q	0.0311573	0.391952
P(R) point	1	1208
R	0	45.65

Result

Type of curve: merged
Comments: Bovine Pancreatic Trypsin Inhibitor (BPTI) was purchased from SIGMA-ALDRICH in the form of lyophilized powder, dissolved with suspension medium and used directly without any further purification. CAUTION! Sample likely aggregated.

	Experimental	Standard	Porod
MW	13.034 kDa	22.36 kDa	3.6 kDa
Volume	-	-	5.8 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	8.437	8.55	0.032
Radius of gyration, Rg	1.42 nm	1.42 nm	0.05

	Min	Max
D	-	4.56
Guinier point	19	218