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- SASDH22: Protein Disulfide Isomerase (PDI) in complex with Bovine Pancreat... -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDH22
SampleProtein Disulfide Isomerase (PDI) in complex with Bovine Pancreatic Trypsin Inhibitor (BPTI)
  • Protein disulfide-isomerase (protein), PDI, Bos taurus
  • Pancreatic trypsin inhibitor (protein), BPTI, Bos taurus
Function / homology
Function and homology information


peptidyl-proline hydroxylation to 4-hydroxy-L-proline / procollagen-proline 4-dioxygenase activity / protein disulfide-isomerase / trypsinogen activation / negative regulation of serine-type endopeptidase activity / sulfate binding / potassium channel inhibitor activity / negative regulation of platelet aggregation / zymogen binding / molecular function inhibitor activity ...peptidyl-proline hydroxylation to 4-hydroxy-L-proline / procollagen-proline 4-dioxygenase activity / protein disulfide-isomerase / trypsinogen activation / negative regulation of serine-type endopeptidase activity / sulfate binding / potassium channel inhibitor activity / negative regulation of platelet aggregation / zymogen binding / molecular function inhibitor activity / protein disulfide isomerase activity / negative regulation of thrombin-activated receptor signaling pathway / serine protease inhibitor complex / response to endoplasmic reticulum stress / serine-type endopeptidase inhibitor activity / melanosome / protein folding / protease binding / endoplasmic reticulum lumen / protein heterodimerization activity / external side of plasma membrane / calcium ion binding / endoplasmic reticulum / extracellular space
Similarity search - Function
Protein disulphide isomerase / Thioredoxin-like domain / Disulphide isomerase / : / Endoplasmic reticulum targeting sequence. / Thioredoxin / Proteinase inhibitor I2, Kunitz, conserved site / Pancreatic trypsin inhibitor (Kunitz) family signature. / BPTI/Kunitz family of serine protease inhibitors. / Pancreatic trypsin inhibitor Kunitz domain ...Protein disulphide isomerase / Thioredoxin-like domain / Disulphide isomerase / : / Endoplasmic reticulum targeting sequence. / Thioredoxin / Proteinase inhibitor I2, Kunitz, conserved site / Pancreatic trypsin inhibitor (Kunitz) family signature. / BPTI/Kunitz family of serine protease inhibitors. / Pancreatic trypsin inhibitor Kunitz domain / Kunitz/Bovine pancreatic trypsin inhibitor domain / Pancreatic trypsin inhibitor (Kunitz) family profile. / Pancreatic trypsin inhibitor Kunitz domain superfamily / Thioredoxin, conserved site / Thioredoxin family active site. / Thioredoxin domain profile. / Thioredoxin domain / Thioredoxin-like superfamily
Similarity search - Domain/homology
Pancreatic trypsin inhibitor / Protein disulfide-isomerase
Similarity search - Component
Biological speciesBos taurus (domestic cattle)
Contact author
  • Chandra Sekar (Pondicherry University)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3882
Type: dummy / Software: (dammif (r7883)) / Radius of dummy atoms: 3.20 A / Symmetry: P1 / Chi-square value: 1.284 / P-value: 0.001454
Model #3883
Type: dummy / Radius of dummy atoms: 3.20 A / Symmetry: P1 / Chi-square value: 1.284 / P-value: 0.001454
Model #3884
Type: dummy / Software: (dammif (r7883)) / Radius of dummy atoms: 3.20 A / Symmetry: P1 / Chi-square value: 1.283 / P-value: 0.001454
Model #3885
Type: dummy / Radius of dummy atoms: 3.20 A / Symmetry: P1 / Chi-square value: 1.283 / P-value: 0.001454
Model #3886
Type: dummy / Software: (DAMFILT 5.0 (r7883)) / Radius of dummy atoms: 2.75 A / Symmetry: P1 / Chi-square value: 1.290 / P-value: 0.001454

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Sample

SampleName: Protein Disulfide Isomerase (PDI) in complex with Bovine Pancreatic Trypsin Inhibitor (BPTI)
Specimen concentration: 2.50-10.00 / Entity id: 1943 / 1944
BufferName: 50 mM Tris-HCL, 150 mM NaCl / pH: 7.5
Entity #1943Name: PDI / Type: protein / Description: Protein disulfide-isomerase / Formula weight: 57.265 / Num. of mol.: 1 / Source: Bos taurus / References: UniProt: P05307
Sequence: MLRRALLCLA LTALFRAGAG APDEEDHVLV LHKGNFDEAL AAHKYLLVEF YAPWCGHCKA LAPEYAKAAG KLKAEGSEIR LAKVDATEES DLAQQYGVRG YPTIKFFKNG DTASPKEYTA GREADDIVNW LKKRTGPAAS TLSDGAAAEA LVESSEVAVI GFFKDMESDS ...Sequence:
MLRRALLCLA LTALFRAGAG APDEEDHVLV LHKGNFDEAL AAHKYLLVEF YAPWCGHCKA LAPEYAKAAG KLKAEGSEIR LAKVDATEES DLAQQYGVRG YPTIKFFKNG DTASPKEYTA GREADDIVNW LKKRTGPAAS TLSDGAAAEA LVESSEVAVI GFFKDMESDS AKQFFLAAEV IDDIPFGITS NSDVFSKYQL DKDGVVLFKK FDEGRNNFEG EVTKEKLLDF IKHNQLPLVI EFTEQTAPKI FGGEIKTHIL LFLPKSVSDY EGKLSNFKKA AESFKGKILF IFIDSDHTDN QRILEFFGLK KEECPAVRLI TLEEEMTKYK PESDELTAEK ITEFCHRFLE GKIKPHLMSQ ELPDDWDKQP VKVLVGKNFE EVAFDEKKNV FVEFYAPWCG HCKQLAPIWD KLGETYKDHE NIVIAKMDST ANEVEAVKVH SFPTLKFFPA SADRTVIDYN GERTLDGFKK FLESGGQDGA GDDDDLEDLE EAEEPDLEED DDQKAVKDEL
Entity #1944Name: BPTI / Type: protein / Description: Pancreatic trypsin inhibitor / Formula weight: 6.517 / Num. of mol.: 1 / Source: Bos taurus / References: UniProt: P00974
Sequence:
RPDFCLEPPY TGPCKARIIR YFYNAKAGLC QTFVYGGCRA KRNNFKSAED CMRTCGGA

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Experimental information

BeamInstrument name: Advanced Photon Source (APS), Argonne National Laboratory BioCAT 18ID
City: Lemont, IL / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 3.485 mm
DetectorName: Pilatus3 X 1M / Pixsize x: 0.172 mm
Scan
Title: Protein Disulfide Isomerase (PDI) in complex with Bovine Pancreatic Trypsin Inhibitor (BPTI)
Measurement date: Nov 4, 2015 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 8 / Unit: 1/A /
MinMax
Q0.0201 0.392
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 780 /
MinMax
Q0.0203962 0.253254
P(R) point1 780
R0 88.5
Result
Type of curve: merged
Comments: PDI was purified from bovine liver. Bovine Pancreatic Trypsin Inhibitor (BPTI) was purchased from SIGMA-ALDRICH in the form of lyophilized powder, dissolved with suspension medium and used ...Comments: PDI was purified from bovine liver. Bovine Pancreatic Trypsin Inhibitor (BPTI) was purchased from SIGMA-ALDRICH in the form of lyophilized powder, dissolved with suspension medium and used directly without any further purification. CAUTION! Sample is likely aggregated.
ExperimentalStandardPorod
MW63.77 kDa62.73 kDa55 kDa
Volume--88 nm3

P(R)GuinierGuinier error
Forward scattering, I029.49 30.83 0.15
Radius of gyration, Rg2.96 nm3.16 nm0.069

MinMax
D-8.85
Guinier point2 71

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