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- SASDGZ9: Protein Disulfide Isomerase (PDI) purified from bovine liver -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDGZ9
SampleProtein Disulfide Isomerase (PDI) purified from bovine liver
  • Protein disulfide-isomerase (protein), PDI, Bos taurus
Function / homology
Function and homology information


peptidyl-proline hydroxylation to 4-hydroxy-L-proline / procollagen-proline 4-dioxygenase activity / protein disulfide-isomerase / protein disulfide isomerase activity / response to endoplasmic reticulum stress / melanosome / protein folding / endoplasmic reticulum lumen / protein heterodimerization activity / external side of plasma membrane / endoplasmic reticulum
Similarity search - Function
Protein disulphide isomerase / Thioredoxin-like domain / Disulphide isomerase / Endoplasmic reticulum targeting sequence. / Thioredoxin / Thioredoxin, conserved site / Thioredoxin family active site. / Thioredoxin domain profile. / Thioredoxin domain / Thioredoxin-like superfamily
Similarity search - Domain/homology
Protein disulfide-isomerase
Similarity search - Component
Biological speciesBos taurus (domestic cattle)
Contact author
  • Chandra Sekar (Pondicherry University)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3863
Type: dummy / Software: (dammif (r7883)) / Radius of dummy atoms: 3.50 A / Symmetry: P1 / Chi-square value: 1.296 / P-value: 0.050249
Model #3874
Type: dummy / Radius of dummy atoms: 3.50 A / Symmetry: P1 / Chi-square value: 1.296 / P-value: 0.050249
Model #3872
Type: dummy / Software: (dammif (r7883)) / Radius of dummy atoms: 3.50 A / Symmetry: P1 / Chi-square value: 1.304 / P-value: 0.050249
Model #3875
Type: dummy / Radius of dummy atoms: 3.50 A / Symmetry: P1 / Chi-square value: 1.304 / P-value: 0.050249
Model #3876
Type: dummy / Software: (DAMFILT 5.0 (r7883)) / Radius of dummy atoms: 2.50 A / Symmetry: P1 / Chi-square value: 1.307 / P-value: 0.050249

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Sample

SampleName: Protein Disulfide Isomerase (PDI) purified from bovine liver
Specimen concentration: 2.50-10.00
BufferName: 50 mM Tris-HCL, 150 mM NaCl / pH: 7.5
Comment: PDI was incubated with 0.5mM TCEP to reduce the Active sites one hour before SAXS data collection
Entity #1937Name: PDI / Type: protein / Description: Protein disulfide-isomerase / Formula weight: 57.265 / Num. of mol.: 1 / Source: Bos taurus / References: UniProt: P05307
Sequence: MLRRALLCLA LTALFRAGAG APDEEDHVLV LHKGNFDEAL AAHKYLLVEF YAPWCGHCKA LAPEYAKAAG KLKAEGSEIR LAKVDATEES DLAQQYGVRG YPTIKFFKNG DTASPKEYTA GREADDIVNW LKKRTGPAAS TLSDGAAAEA LVESSEVAVI GFFKDMESDS ...Sequence:
MLRRALLCLA LTALFRAGAG APDEEDHVLV LHKGNFDEAL AAHKYLLVEF YAPWCGHCKA LAPEYAKAAG KLKAEGSEIR LAKVDATEES DLAQQYGVRG YPTIKFFKNG DTASPKEYTA GREADDIVNW LKKRTGPAAS TLSDGAAAEA LVESSEVAVI GFFKDMESDS AKQFFLAAEV IDDIPFGITS NSDVFSKYQL DKDGVVLFKK FDEGRNNFEG EVTKEKLLDF IKHNQLPLVI EFTEQTAPKI FGGEIKTHIL LFLPKSVSDY EGKLSNFKKA AESFKGKILF IFIDSDHTDN QRILEFFGLK KEECPAVRLI TLEEEMTKYK PESDELTAEK ITEFCHRFLE GKIKPHLMSQ ELPDDWDKQP VKVLVGKNFE EVAFDEKKNV FVEFYAPWCG HCKQLAPIWD KLGETYKDHE NIVIAKMDST ANEVEAVKVH SFPTLKFFPA SADRTVIDYN GERTLDGFKK FLESGGQDGA GDDDDLEDLE EAEEPDLEED DDQKAVKDEL

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Experimental information

BeamInstrument name: Advanced Photon Source (APS), Argonne National Laboratory BioCAT 18ID
City: Lemont, IL / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 3.485 mm
DetectorName: Pilatus3 X 1M / Pixsize x: 0.172 mm
Scan
Title: Protein Disulfide Isomerase (PDI) purified from bovine liver
Measurement date: Nov 4, 2015 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 8 / Unit: 1/A /
MinMax
Q0.0105 0.392
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 856 /
MinMax
Q0.0153146 0.27089
P(R) point1 856
R0 97.82
Result
Type of curve: merged
Comments: PDI was purified from bovine liver and was incubated with 0.5mM TCEP to reduce the active sites one hour before SAXS data collection.
ExperimentalStandardPorod
MW57 kDa58 kDa61 kDa
Volume--98 nm3

P(R)GuinierGuinier error
Forward scattering, I029.68 29.8 0.072
Radius of gyration, Rg2.95 nm2.95 nm0.09

MinMax
D-9.78
Guinier point17 113

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