SASDGZ5: Suppressor of Fused from Human D30 (Suppressor of fused homolog, ...

Basic information

• Entry: Database: SASBDB / ID: SASDGZ5

Sample

Suppressor of Fused from Human D30

• Suppressor of fused homolog (protein), hSuFuD30, Homo sapiens

Function / homology

Function:

smoothened signaling pathway involved in ventral spinal cord interneuron specification / smoothened signaling pathway involved in spinal cord motor neuron cell fate specification / positive regulation of cellular response to drug / GLI-SUFU complex / ciliary tip / coronary vasculature development / aorta development / ventricular septum development / negative regulation of protein import into nucleus / skin development / ciliary base / spermatid development / heart looping / negative regulation of ubiquitin-dependent protein catabolic process / negative regulation of osteoblast differentiation / Hedgehog 'off' state / negative regulation of smoothened signaling pathway / negative regulation of DNA-binding transcription factor activity / neural tube closure / Degradation of GLI1 by the proteasome / Hedgehog 'on' state / Degradation of GLI2 by the proteasome / GLI3 is processed to GLI3R by the proteasome / beta-catenin binding / transcription corepressor activity / cilium / regulation of DNA-templated transcription / protein kinase binding / negative regulation of transcription by RNA polymerase II / signal transduction / nucleoplasm / nucleus / cytosol / cytoplasm

Domain/homology:

Suppressor of fused / Suppressor of fused, eukaryotic / Suppressor of fused C-terminal / Suppressor of fused, N-terminal / Suppressor of fused, C-terminal domain superfamily / Suppressor of Fused Gli/Ci N terminal binding domain / Suppressor of fused-like domain / Suppressor of fused protein (SUFU)

Component:

Suppressor of fused homolog

• Biological species: Homo sapiens (human)

Contact author

• Valerie Biou (IBPC-CNRS)

Models

• Model #3802: ; Type: atomic / Symmetry: P1; Comment: human SuFu truncated from 30aa. body1 = A: 30 - 255 body2 = A: 259 - 268, A: 356 - 472; Chi-square value: 3.087049 / P-value: 0.000025

Sample

• Sample: Name: Suppressor of Fused from Human D30 / Specimen concentration: 14 mg/ml
• Buffer: Name: 50 mM bis-TRIS pH 5.5, 200 mM NaCl, 10% glycerol / pH: 5.5

Entity #1907

Name: hSuFuD30 / Type: protein / Description: Suppressor of fused homolog / Formula weight: 51.09 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q9UMX1
Sequence:

PPGLHAIYGE CRRLYPDQPN PLQVTAIVKY WLGGPDPLDY VSMYRNVGSP SANIPEHWHY ISFGLSDLYG DNRVHEFTGT DGPSGFGFEL TFRLKRETGE SAPPTWPAEL MQGLARYVFQ SENTFCSGDH VSWHSPLDNS ESRIQHMLLT EDPQMQPVQT PFGVVTFLQI VGVCTEELHS AQQWNGQGIL ELLRTVPIAG GPWLITDMRR GETIFEIDPH LQERVDKGIE TDGSNLSGVS AKCAWDDLSR PPEDDEDSRS ICIGTQPRRL SGKDTEQIRE TLRRGLEINS KPVLPPINPQ RQNGLAHDRA PSRKDSLESD SSTAIIPHEL IRTRQLESVH LKFNQESGAL IPLCLRGRLL HGRHFTYKSI TGDMAITFVS TGVEGAFATE EHPYAAHGPW LQILLTEEFV EKMLEDLEDL TSPEEFKLPK EYSWPEKKLK VSILPDVVFD SPLH

Experimental information

• Beam: Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 2.09 mm
• Detector: Name: Eiger 4M / Pixsize x: 75 mm

Scan

Title: Suppressor of Fused from Human D30 / Measurement date: Jun 30, 2018 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 0.99 sec. / Number of frames: 200 / Unit: 1/A /

	Min	Max
Q	0.0036	0.5592

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 506 /

	Min	Max
Q	0.0164195	0.246598
P(R) point	1	506
R	0	140

Result

Experimental MW: 51 kDa / Type of curve: sec

	P(R)	Guinier	Guinier error
Forward scattering, I0	2.307	2.25	0.005
Radius of gyration, Rg	3.46 nm	3.18 nm	0.01

	Min	Max
D	-	14
Guinier point	32	82