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- SASDGY9: Nucleolar RNA helicase 2 (DDX21) fragment 186-783 monomeric mutant -

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Basic information

Entry
Database: SASBDB / ID: SASDGY9
SampleNucleolar RNA helicase 2 (DDX21) fragment 186-783 monomeric mutant
  • Nucleolar RNA helicase 2 (protein), DDX21, Homo sapiens
Function / homology
Function and homology information


RNA polymerase inhibitor activity / 7SK snRNA binding / R-loop processing / positive regulation of myeloid dendritic cell cytokine production / B-WICH complex / miRNA binding / negative regulation of transcription by RNA polymerase I / positive regulation of transcription by RNA polymerase III / snoRNA binding / response to exogenous dsRNA ...RNA polymerase inhibitor activity / 7SK snRNA binding / R-loop processing / positive regulation of myeloid dendritic cell cytokine production / B-WICH complex / miRNA binding / negative regulation of transcription by RNA polymerase I / positive regulation of transcription by RNA polymerase III / snoRNA binding / response to exogenous dsRNA / positive regulation of transcription by RNA polymerase I / Major pathway of rRNA processing in the nucleolus and cytosol / B-WICH complex positively regulates rRNA expression / rRNA processing / osteoblast differentiation / double-stranded RNA binding / chromosome / defense response to virus / transcription by RNA polymerase II / RNA helicase activity / positive regulation of canonical NF-kappaB signal transduction / rRNA binding / RNA helicase / chromatin remodeling / innate immune response / mRNA binding / nucleolus / positive regulation of transcription by RNA polymerase II / ATP hydrolysis activity / mitochondrion / RNA binding / nucleoplasm / ATP binding / identical protein binding / membrane / cytosol
Similarity search - Function
GUCT / GUCT (NUC152) domain / : / DEAD-box RNA helicase Q motif profile. / DEAD/DEAH box helicase domain / DEAD/DEAH box helicase / RNA-binding domain superfamily / Helicase conserved C-terminal domain / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. ...GUCT / GUCT (NUC152) domain / : / DEAD-box RNA helicase Q motif profile. / DEAD/DEAH box helicase domain / DEAD/DEAH box helicase / RNA-binding domain superfamily / Helicase conserved C-terminal domain / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Nucleolar RNA helicase 2
Similarity search - Component
Biological speciesHomo sapiens (human)
Contact author
  • Maria Josefina Marcaida (École polytechnique fédérale de Lausanne (EPFL), Switzerland)

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Structure visualization

Downloads & links

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Models

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Sample

SampleName: Nucleolar RNA helicase 2 (DDX21) fragment 186-783 monomeric mutant
Specimen concentration: 10 mg/ml
BufferName: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP / pH: 7.5
Entity #1961Name: DDX21 / Type: protein / Description: Nucleolar RNA helicase 2 / Formula weight: 69.181 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q9NR30
Sequence: MGSSHHHHHH SQDPENLYFQ GAFSNFPISE ETIKLLKGRG VTFLFPIQAK TFHHVYSGKD LIAQARTGTG KTFSFAIPLI EKLHGELQDR KRGRAPQVLV LAPTRELANQ VSKDFSDITK KLSVACFYGG TPYGGQFERM RNGIDILVGT PGRIKDHIQN GKLDLTKLKH ...Sequence:
MGSSHHHHHH SQDPENLYFQ GAFSNFPISE ETIKLLKGRG VTFLFPIQAK TFHHVYSGKD LIAQARTGTG KTFSFAIPLI EKLHGELQDR KRGRAPQVLV LAPTRELANQ VSKDFSDITK KLSVACFYGG TPYGGQFERM RNGIDILVGT PGRIKDHIQN GKLDLTKLKH VVLDEVDQML DMGFADQVEE ILSVAYKKDS EDNPQTLLFS ATCPHWVFNV AKKYMKSTYE QVDLIGKKTQ KTAITVEHLA IKCHWTQRAA VIGDVIRVYS GHQGRTIIFC ETKKEAQELS QNSAIKQDAQ SLHGDIPQKQ REITLKGFRN GSFGVLVATN VAARGLDIPE VDLVIQSSPP KDVESYIHRS GRTGRAGRTG VCICFYQHKE EYQLVQVEQK AGIKFKRIGV PSATEIIKAS SKDAIRLLDS VPPTAISHFK QSAEKLIEEK GAVEARRRRR RHISGATSVD QRSLINSNVG FVTMILQCSI EMPNISYAWK ELKEQLGEEI DSKVKGMVFL KGKLGVCFDV PTASVTEIQE KWHDSRRWQL SVATEQPELE GPREGYGGFR GQREGSRGFR GQRDGNRRFR GQREGSRGPR GQRSGGGNKS NRSQNKGQKR SFSKAFGQ

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Experimental information

BeamInstrument name: ESRF BioSAXS beamline BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.099 Å / Dist. spec. to detc.: 2.867 mm
DetectorName: d / Type: PILATUS 1 M, DECTRIS / Pixsize x: 0.99 mm
Scan
Title: Nucleolar RNA helicase 2 (DDX21) fragment 186-783 monomeric mutant
Measurement date: Nov 3, 2018 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 2160 / Unit: 1/A /
MinMax
Q0.0134 0.3801
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 779 /
MinMax
Q0.0134336 0.380099
P(R) point1 779
R0 177
Result
Type of curve: sec /
ExperimentalPorod
MW68 kDa65 kDa
Volume-111 nm3

P(R)GuinierGuinier error
Forward scattering, I045.74 44.65 0.2
Radius of gyration, Rg4.4 nm4.07 nm0.03

MinMax
D-17.7
Guinier point1 40

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