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- SASDGX9: Nucleolar RNA helicase 2 (DDX21) fragment 186-620 (Nucleolar RNA ... -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDGX9
SampleNucleolar RNA helicase 2 (DDX21) fragment 186-620
  • Nucleolar RNA helicase 2 fragment 186-620 (protein), DDX21-186-620, Homo sapiens
Function / homology
Function and homology information


RNA polymerase inhibitor activity / 7SK snRNA binding / R-loop processing / central nervous system projection neuron axonogenesis / positive regulation of myeloid dendritic cell cytokine production / B-WICH complex / miRNA binding / positive regulation of transcription by RNA polymerase III / negative regulation of transcription by RNA polymerase I / snoRNA binding ...RNA polymerase inhibitor activity / 7SK snRNA binding / R-loop processing / central nervous system projection neuron axonogenesis / positive regulation of myeloid dendritic cell cytokine production / B-WICH complex / miRNA binding / positive regulation of transcription by RNA polymerase III / negative regulation of transcription by RNA polymerase I / snoRNA binding / positive regulation of transcription by RNA polymerase I / response to exogenous dsRNA / Major pathway of rRNA processing in the nucleolus and cytosol / neuron projection maintenance / B-WICH complex positively regulates rRNA expression / microtubule cytoskeleton organization / osteoblast differentiation / rRNA processing / double-stranded RNA binding / chromosome / positive regulation of canonical NF-kappaB signal transduction / defense response to virus / transcription by RNA polymerase II / RNA helicase activity / rRNA binding / RNA helicase / chromatin remodeling / innate immune response / mRNA binding / nucleolus / ATP hydrolysis activity / positive regulation of transcription by RNA polymerase II / mitochondrion / RNA binding / nucleoplasm / ATP binding / membrane / identical protein binding / cytosol
Similarity search - Function
GUCT / GUCT (NUC152) domain / DEAD-box RNA helicase Q motif profile. / DEAD/DEAH box helicase / DEAD/DEAH box helicase domain / Helicase conserved C-terminal domain / RNA-binding domain superfamily / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. ...GUCT / GUCT (NUC152) domain / DEAD-box RNA helicase Q motif profile. / DEAD/DEAH box helicase / DEAD/DEAH box helicase domain / Helicase conserved C-terminal domain / RNA-binding domain superfamily / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Nucleolar RNA helicase 2
Similarity search - Component
Biological speciesHomo sapiens (human)
Contact author
  • Maria Josefina Marcaida (École polytechnique fédérale de Lausanne (EPFL), Switzerland)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2926
Type: dummy / Software: (r10552) / Radius of dummy atoms: 2.50 A / Symmetry: P1 / Chi-square value: 1.071 / P-value: 0.317391

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Sample

SampleName: Nucleolar RNA helicase 2 (DDX21) fragment 186-620 / Specimen concentration: 10 mg/ml
BufferName: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP / pH: 7.5
Entity #1349Name: DDX21-186-620 / Type: protein / Description: Nucleolar RNA helicase 2 fragment 186-620 / Formula weight: 49.034 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: Q9NR30
Sequence: MGAFSNFPIS EETIKLLKGR GVTFLFPIQA KTFHHVYSGK DLIAQARTGT GKTFSFAIPL IEKLHGELQD RKRGRAPQVL VLAPTRELAN QVSKDFSDIT KKLSVACFYG GTPYGGQFER MRNGIDILVG TPGRIKDHIQ NGKLDLTKLK HVVLDEVDQM LDMGFADQVE ...Sequence:
MGAFSNFPIS EETIKLLKGR GVTFLFPIQA KTFHHVYSGK DLIAQARTGT GKTFSFAIPL IEKLHGELQD RKRGRAPQVL VLAPTRELAN QVSKDFSDIT KKLSVACFYG GTPYGGQFER MRNGIDILVG TPGRIKDHIQ NGKLDLTKLK HVVLDEVDQM LDMGFADQVE EILSVAYKKD SEDNPQTLLF SATCPHWVFN VAKKYMKSTY EQVDLIGKKT QKTAITVEHL AIKCHWTQRA AVIGDVIRVY SGHQGRTIIF CETKKEAQEL SQNSAIKQDA QSLHGDIPQK QREITLKGFR NGSFGVLVAT NVAARGLDIP EVDLVIQSSP PKDVESYIHR SGRTGRAGRT GVCICFYQHK EEYQLVQVEQ KAGIKFKRIG VPSATEIIKA SSKDAIRLLD SVPPTAISHF KQSAEKLIEE KGAVEALAAA LAHISGHHHH HH

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.099 Å / Dist. spec. to detc.: 2.867 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Nucleolar RNA helicase 2 (DDX21) fragment 186-620 / Measurement date: Mar 4, 2018 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 3000 / Unit: 1/A /
MinMax
Q0.0098 0.3787
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 787 /
MinMax
Q0.00982689 0.378722
P(R) point1 787
R0 169
Result
Type of curve: sec /
ExperimentalPorod
MW93 kDa94 kDa
Volume-150 nm3

P(R)GuinierGuinier error
Forward scattering, I055.97 55.51 0.092
Radius of gyration, Rg4.59 nm4.42 nm0.03

MinMax
D-16.9
Guinier point1 39

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