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- SASDGV5: The nucleotide binding domain of Lipid A export ATP-binding/perme... -

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Basic information

Entry
Database: SASBDB / ID: SASDGV5
SampleThe nucleotide binding domain of Lipid A export ATP-binding/permease protein MsbA - data from stop-and-flow time-resolved SAXS (12 s time course)
  • Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain (protein), MsbA, Escherichia coli (strain K12)
Function / homology
Function and homology information


MsbA transporter complex / lipopolysaccharide floppase activity / lipid translocation / ABC-type lipid A-core oligosaccharide transporter / lipopolysaccharide transport / ATPase-coupled lipid transmembrane transporter activity / ABC-type xenobiotic transporter activity / lipid transport / ATP-binding cassette (ABC) transporter complex / transmembrane transport ...MsbA transporter complex / lipopolysaccharide floppase activity / lipid translocation / ABC-type lipid A-core oligosaccharide transporter / lipopolysaccharide transport / ATPase-coupled lipid transmembrane transporter activity / ABC-type xenobiotic transporter activity / lipid transport / ATP-binding cassette (ABC) transporter complex / transmembrane transport / lipid binding / ATP hydrolysis activity / ATP binding / membrane / identical protein binding / plasma membrane
Similarity search - Function
Lipid A export ATP-binding/permease protein msbA family profile. / ABC transporter, lipid A-core flippase, MsbA / Type 1 protein exporter / ABC transporter transmembrane region / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter ...Lipid A export ATP-binding/permease protein msbA family profile. / ABC transporter, lipid A-core flippase, MsbA / Type 1 protein exporter / ABC transporter transmembrane region / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
ATP-dependent lipid A-core flippase
Similarity search - Component
Biological speciesEscherichia coli (strain K12) (bacteria)
Contact author
  • Clement Blanchet (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Sample

SampleName: The nucleotide binding domain of Lipid A export ATP-binding/permease protein MsbA - data from stop-and-flow time-resolved SAXS (12 s time course)
BufferName: 20 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.45 mM Mg2+-ATP / pH: 7.5
Entity #1911Name: MsbA / Type: protein
Description: Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain
Formula weight: 27.107 / Num. of mol.: 1 / Source: Escherichia coli (strain K12) / References: UniProt: P60752
Sequence: ATGDVEFRNV TFTYPGRDVP ALRNINLKIP AGKTVALVGR SGSGKSTIAS LITRFYDIDE GEILMDGHDL REYTLASLRN QVALVSQNVH LFNDTVANNI AYARTEQYSR EQIEEAARMA YAMDFINKMD NGLDTVIGEN GVLLSGGQRQ RIAIARALLR DSPILILDEA ...Sequence:
ATGDVEFRNV TFTYPGRDVP ALRNINLKIP AGKTVALVGR SGSGKSTIAS LITRFYDIDE GEILMDGHDL REYTLASLRN QVALVSQNVH LFNDTVANNI AYARTEQYSR EQIEEAARMA YAMDFINKMD NGLDTVIGEN GVLLSGGQRQ RIAIARALLR DSPILILDEA TSALDTESER AIQAALDELQ KNRTSLVIAH RLSTIEKADE IVVVEDGVIV ERGTHNDLLE HRGVYAQLHK MQFGQ

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
Scan
Title: The nucleotide binding domain of Lipid A export ATP-binding/permease protein MsbA - data from stop-and-flow time-resolved SAXS (12 s time course)
Measurement date: Dec 8, 2017 / Cell temperature: 20 °C / Exposure time: 0.03 sec. / Number of frames: 400 / Unit: 1/nm /
MinMax
Q0.0273 5.0671
ResultType of curve: other
Comments: The SAXS curve displayed in this entry corresponds to the last time point (12 s) of a stop-and-flow SAXS experiment monitoring the disassociation of NBD dimers into monomers caused by the ...Comments: The SAXS curve displayed in this entry corresponds to the last time point (12 s) of a stop-and-flow SAXS experiment monitoring the disassociation of NBD dimers into monomers caused by the introduction and subsequent hydrolysis of ATP. At 12 seconds through the time course the NBD becomes predominantly monomeric, with trace amounts of dimer. Attached to this entry, and made available in the full-entry zip archive, are all of the time-resolved stop-and-flow SAXS data out to the final 12 s time point.
ExperimentalPorod
MW33 kDa-
Volume-50 nm3

GuinierGuinier error
Forward scattering, I030582 80
Radius of gyration, Rg2.12 nm0.02

MinMax
D-6.79
Guinier point46 212

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