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- SASDGU9: Nucleolar RNA helicase 2 (DDX21-FL) (Nucleolar RNA helicase 2, DDX21) -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDGU9
SampleNucleolar RNA helicase 2 (DDX21-FL)
  • Nucleolar RNA helicase 2 (protein), DDX21, Homo sapiens
Function / homology
Function and homology information


RNA polymerase inhibitor activity / 7SK snRNA binding / R-loop processing / positive regulation of myeloid dendritic cell cytokine production / B-WICH complex / : / miRNA binding / positive regulation of transcription by RNA polymerase III / negative regulation of transcription by RNA polymerase I / snoRNA binding ...RNA polymerase inhibitor activity / 7SK snRNA binding / R-loop processing / positive regulation of myeloid dendritic cell cytokine production / B-WICH complex / : / miRNA binding / positive regulation of transcription by RNA polymerase III / negative regulation of transcription by RNA polymerase I / snoRNA binding / response to exogenous dsRNA / positive regulation of transcription by RNA polymerase I / Major pathway of rRNA processing in the nucleolus and cytosol / B-WICH complex positively regulates rRNA expression / rRNA processing / osteoblast differentiation / double-stranded RNA binding / chromosome / positive regulation of canonical NF-kappaB signal transduction / defense response to virus / transcription by RNA polymerase II / RNA helicase activity / rRNA binding / RNA helicase / chromatin remodeling / innate immune response / nucleolus / ATP hydrolysis activity / positive regulation of transcription by RNA polymerase II / mitochondrion / RNA binding / nucleoplasm / ATP binding / membrane / identical protein binding / cytosol
Similarity search - Function
GUCT / GUCT (NUC152) domain / DEAD-box RNA helicase Q motif profile. / DEAD/DEAH box helicase / DEAD/DEAH box helicase domain / Helicase conserved C-terminal domain / RNA-binding domain superfamily / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. ...GUCT / GUCT (NUC152) domain / DEAD-box RNA helicase Q motif profile. / DEAD/DEAH box helicase / DEAD/DEAH box helicase domain / Helicase conserved C-terminal domain / RNA-binding domain superfamily / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Nucleolar RNA helicase 2
Similarity search - Component
Biological speciesHomo sapiens (human)
Contact author
  • Maria Josefina Marcaida (École polytechnique fédérale de Lausanne (EPFL), Switzerland)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2551
Type: dummy / Software: (r9988) / Radius of dummy atoms: 2.10 A / Symmetry: P1 / Chi-square value: 1.093 / P-value: 0.091176

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Sample

SampleName: Nucleolar RNA helicase 2 (DDX21-FL) / Specimen concentration: 10 mg/ml
BufferName: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP / pH: 7.5
Entity #1324Name: DDX21 / Type: protein / Description: Nucleolar RNA helicase 2 / Formula weight: 90.993 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: Q9NR30
Sequence: GSGMKETAAA KFERQHMDSP DLGTDDDDKA MADIGSPGKL RSDAGLESDT AMKKGETLRK QTEEKEKKEK PKSDKTEEIA EEEETVFPKA KQVKKKAEPS EVDMNSPKSK KAKKKEEPSQ NDISPKTKSL RKKKEPIEKK VVSSKTKKVT KNEEPSEEEI DAPKPKKMKK ...Sequence:
GSGMKETAAA KFERQHMDSP DLGTDDDDKA MADIGSPGKL RSDAGLESDT AMKKGETLRK QTEEKEKKEK PKSDKTEEIA EEEETVFPKA KQVKKKAEPS EVDMNSPKSK KAKKKEEPSQ NDISPKTKSL RKKKEPIEKK VVSSKTKKVT KNEEPSEEEI DAPKPKKMKK EKEMNGETRE KSPKLKNGFP HPEPDCNPSE AASEESNSEI EQEIPVEQKE GAFSNFPISE ETIKLLKGRG VTFLFPIQAK TFHHVYSGKD LIAQARTGTG KTFSFAIPLI EKLHGELQDR KRGRAPQVLV LAPTRELANQ VSKDFSDITK KLSVACFYGG TPYGGQFERM RNGIDILVGT PGRIKDHIQN GKLDLTKLKH VVLDEVDQML DMGFADQVEE ILSVAYKKDS EDNPQTLLFS ATCPHWVFNV AKKYMKSTYE QVDLIGKKTQ KTAITVEHLA IKCHWTQRAA VIGDVIRVYS GHQGRTIIFC ETKKEAQELS QNSAIKQDAQ SLHGDIPQKQ REITLKGFRN GSFGVLVATN VAARGLDIPE VDLVIQSSPP KDVESYIHRS GRTGRAGRTG VCICFYQHKE EYQLVQVEQK AGIKFKRIGV PSATEIIKAS SKDAIRLLDS VPPTAISHFK QSAEKLIEEK GAVEALAAAL AHISGATSVD QRSLINSNVG FVTMILQCSI EMPNISYAWK ELKEQLGEEI DSKVKGMVFL KGKLGVCFDV PTASVTEIQE KWHDSRRWQL SVATEQPELE GPREGYGGFR GQREGSRGFR GQRDGNRRFR GQREGSRGPR GQRSGGGNKS NRSQNKGQKR SFSKAFGQ

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.099 Å / Dist. spec. to detc.: 2.867 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Nucleolar RNA helicase 2 (DDX21-FL) / Measurement date: Nov 25, 2017 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 3000 / Unit: 1/A /
MinMax
Q0.0071 0.3792
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 793 /
MinMax
Q0.00713852 0.379197
P(R) point1 793
R0 319
Result
Type of curve: sec /
ExperimentalPorod
MW226 kDa425 kDa
Volume-681 nm3

P(R)GuinierGuinier error
Forward scattering, I066.45 64.97 0.19
Radius of gyration, Rg7.59 nm6.99 nm0.032

MinMax
D-31.9
Guinier point1 22

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