SASDGU7: Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin

基本情報

登録情報

データベース: SASBDB / ID: SASDGU7

試料

Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin

• Heparin (other)
• Apolipoprotein E4 (1-191) (protein), ApoE4, Homo sapiens

機能・相同性

分子機能:

lipid transport involved in lipid storage / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / triglyceride-rich lipoprotein particle clearance / lipoprotein particle / discoidal high-density lipoprotein particle / negative regulation of cholesterol biosynthetic process / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / regulation of amyloid-beta clearance / positive regulation of lipoprotein transport / chylomicron remnant clearance / chylomicron remnant / maintenance of location in cell / intermediate-density lipoprotein particle / very-low-density lipoprotein particle remodeling / acylglycerol homeostasis / NMDA glutamate receptor clustering / very-low-density lipoprotein particle clearance / negative regulation of triglyceride metabolic process / phosphatidylcholine-sterol O-acyltransferase activator activity / response to caloric restriction / Chylomicron clearance / positive regulation of phospholipid efflux / Chylomicron remodeling / positive regulation of cholesterol metabolic process / cellular response to lipoprotein particle stimulus / regulation of amyloid fibril formation / regulation of behavioral fear response / positive regulation of low-density lipoprotein particle receptor catabolic process / regulation of protein metabolic process / Chylomicron assembly / high-density lipoprotein particle clearance / high-density lipoprotein particle remodeling / lipid transporter activity / phospholipid efflux / chylomicron / lipoprotein catabolic process / very-low-density lipoprotein particle receptor binding / AMPA glutamate receptor clustering / multivesicular body, internal vesicle / melanosome organization / reverse cholesterol transport / high-density lipoprotein particle assembly / positive regulation of amyloid-beta clearance / cholesterol transfer activity / positive regulation by host of viral process / protein import / positive regulation of CoA-transferase activity / low-density lipoprotein particle / lipoprotein biosynthetic process / very-low-density lipoprotein particle / high-density lipoprotein particle / low-density lipoprotein particle remodeling / negative regulation of amyloid fibril formation / heparan sulfate proteoglycan binding / regulation of Cdc42 protein signal transduction / amyloid precursor protein metabolic process / triglyceride homeostasis / synaptic transmission, cholinergic / regulation of amyloid precursor protein catabolic process / positive regulation of membrane protein ectodomain proteolysis / cholesterol catabolic process / HDL remodeling / negative regulation of protein metabolic process / negative regulation of endothelial cell migration / cholesterol efflux / artery morphogenesis / regulation of axon extension / Scavenging by Class A Receptors / regulation of cholesterol metabolic process / triglyceride metabolic process / positive regulation of dendritic spine development / positive regulation of amyloid fibril formation / low-density lipoprotein particle receptor binding / regulation of innate immune response / virion assembly / negative regulation of endothelial cell proliferation / locomotory exploration behavior / negative regulation of amyloid-beta formation / antioxidant activity / regulation of neuronal synaptic plasticity / positive regulation of endocytosis / lipoprotein particle binding / response to dietary excess / negative regulation of blood vessel endothelial cell migration / negative regulation of long-term synaptic potentiation / positive regulation of dendritic spine maintenance / negative regulation of platelet activation / positive regulation of cholesterol efflux / regulation of protein-containing complex assembly / negative regulation of protein secretion / negative regulation of blood coagulation / intracellular transport / fatty acid homeostasis / long-term memory / synaptic cleft / long-chain fatty acid transport / positive regulation of lipid biosynthetic process

ドメイン・相同性:

Apolipoprotein A/E / : / Apolipoprotein A1/A4/E domain

構成要素:

Apolipoprotein E

• 生物種: Homo sapiens (ヒト)

登録者

• Lucas Kraft (University of Sussex, Brighton, UK)

モデル

試料

• 試料: 名称: Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin; 試料濃度: 4 mg/ml / Entity id: 1633 / 1635
• バッファ: 名称: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

要素 #1633

タイプ: other / 記述: Heparin / 分子量: 15 / 分子数: 1
配列:

(C12H19NO20S3)n

要素 #1635

名称: ApoE4 / タイプ: protein / 記述: Apolipoprotein E4 (1-191) / 分子量: 22.605 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P02649
配列:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVRGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KRLAVYQAGA REGAERGLSA IRERLGPLVE QGRVR

実験情報

• ビーム: 設備名称: Diamond Light Source B21 / 地域: Didcot / 国: UK / 形状: 1 x 5 mm / 線源: X-ray synchrotron / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 4.036 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin
測定日: 2018年5月3日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 28 / 単位: 1/A /

	Min	Max
Q	0.003	0.37

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 1102 /

	Min	Max
Q	0.0248108	0.269848
P(R) point	1	1102
R	0	105

結果

Experimental MW: 33 kDa / カーブのタイプ: single_conc
コメント: ApoE4 (1-191) + 330 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.

	P(R)	Guinier	Guinier error
前方散乱 I0	0.1085	0.11	0.0017
慣性半径, Rg	3.13 nm	3.135 nm	0.067

	Min	Max
D	-	10.5
Guinier point	99	166