SASDGU7: Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin

Basic information

Entry

Database: SASBDB / ID: SASDGU7

Sample

Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin

• Heparin (other)
• Apolipoprotein E4 (1-191) (protein), ApoE4, Homo sapiens

Function / homology

Function:

lipid transport involved in lipid storage / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / triglyceride-rich lipoprotein particle clearance / discoidal high-density lipoprotein particle / lipoprotein particle / negative regulation of triglyceride metabolic process / negative regulation of cholesterol biosynthetic process / regulation of amyloid-beta clearance / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / positive regulation of lipoprotein transport / chylomicron remnant clearance / chylomicron remnant / maintenance of location in cell / intermediate-density lipoprotein particle / very-low-density lipoprotein particle remodeling / acylglycerol homeostasis / NMDA glutamate receptor clustering / response to caloric restriction / very-low-density lipoprotein particle clearance / phosphatidylcholine-sterol O-acyltransferase activator activity / Chylomicron clearance / positive regulation of phospholipid efflux / lipid transporter activity / Chylomicron remodeling / positive regulation of cholesterol metabolic process / regulation of amyloid fibril formation / regulation of behavioral fear response / cellular response to lipoprotein particle stimulus / positive regulation of low-density lipoprotein particle receptor catabolic process / regulation of protein metabolic process / Chylomicron assembly / high-density lipoprotein particle clearance / high-density lipoprotein particle remodeling / chylomicron / phospholipid efflux / AMPA glutamate receptor clustering / very-low-density lipoprotein particle receptor binding / lipoprotein catabolic process / multivesicular body, internal vesicle / melanosome organization / reverse cholesterol transport / high-density lipoprotein particle assembly / positive regulation of amyloid-beta clearance / positive regulation by host of viral process / : / low-density lipoprotein particle / protein import / lipoprotein biosynthetic process / cholesterol transfer activity / high-density lipoprotein particle / very-low-density lipoprotein particle / low-density lipoprotein particle remodeling / heparan sulfate proteoglycan binding / negative regulation of amyloid fibril formation / regulation of Cdc42 protein signal transduction / amyloid precursor protein metabolic process / triglyceride homeostasis / regulation of amyloid precursor protein catabolic process / cholesterol catabolic process / positive regulation of membrane protein ectodomain proteolysis / synaptic transmission, cholinergic / HDL remodeling / negative regulation of endothelial cell migration / cholesterol efflux / regulation of axon extension / artery morphogenesis / Scavenging by Class A Receptors / negative regulation of protein metabolic process / regulation of cholesterol metabolic process / positive regulation of dendritic spine development / triglyceride metabolic process / low-density lipoprotein particle receptor binding / positive regulation of amyloid fibril formation / regulation of innate immune response / virion assembly / negative regulation of amyloid-beta formation / locomotory exploration behavior / negative regulation of endothelial cell proliferation / antioxidant activity / positive regulation of endocytosis / lipoprotein particle binding / response to dietary excess / regulation of neuronal synaptic plasticity / negative regulation of blood vessel endothelial cell migration / negative regulation of long-term synaptic potentiation / negative regulation of platelet activation / positive regulation of cholesterol efflux / positive regulation of dendritic spine maintenance / negative regulation of blood coagulation / negative regulation of MAP kinase activity / long-term memory / negative regulation of protein secretion / intracellular transport / fatty acid homeostasis / regulation of protein-containing complex assembly / long-chain fatty acid transport / synaptic cleft

Domain/homology:

Apolipoprotein A/E / : / Apolipoprotein A1/A4/E domain

Component:

Apolipoprotein E

• Biological species: Homo sapiens (human)

Contact author

• Lucas Kraft (University of Sussex, Brighton, UK)

Models

Sample

• Sample: Name: Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin; Specimen concentration: 4 mg/ml / Entity id: 1633 / 1635
• Buffer: Name: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

Entity #1633

Type: other / Description: Heparin / Formula weight: 15 / Num. of mol.: 1
Sequence:

(C12H19NO20S3)n

Entity #1635

Name: ApoE4 / Type: protein / Description: Apolipoprotein E4 (1-191) / Formula weight: 22.605 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P02649
Sequence:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVRGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KRLAVYQAGA REGAERGLSA IRERLGPLVE QGRVR

Experimental information

• Beam: Instrument name: Diamond Light Source B21 / City: Didcot / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.036 mm
• Detector: Name: Pilatus 2M

Scan

Title: Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin
Measurement date: May 3, 2018 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 28 / Unit: 1/A /

	Min	Max
Q	0.003	0.37

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 1102 /

	Min	Max
Q	0.0248108	0.269848
P(R) point	1	1102
R	0	105

Result

Experimental MW: 33 kDa / Type of curve: single_conc
Comments: ApoE4 (1-191) + 330 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.1085	0.11	0.0017
Radius of gyration, Rg	3.13 nm	3.135 nm	0.067

	Min	Max
D	-	10.5
Guinier point	99	166