SASDGT8: Apolipoprotein E4 (methylated) bound to 37 µM Suramin

基本情報

登録情報

データベース: SASBDB / ID: SASDGT8

試料

Apolipoprotein E4 (methylated) bound to 37 µM Suramin

• Apolipoprotein E4 (protein), ApoE4, Homo sapiens
• Suramin (other)

機能・相同性

分子機能:

lipid transport involved in lipid storage / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / triglyceride-rich lipoprotein particle clearance / discoidal high-density lipoprotein particle / lipoprotein particle / negative regulation of triglyceride metabolic process / negative regulation of cholesterol biosynthetic process / regulation of amyloid-beta clearance / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / positive regulation of lipoprotein transport / chylomicron remnant clearance / chylomicron remnant / maintenance of location in cell / intermediate-density lipoprotein particle / very-low-density lipoprotein particle remodeling / acylglycerol homeostasis / NMDA glutamate receptor clustering / response to caloric restriction / very-low-density lipoprotein particle clearance / phosphatidylcholine-sterol O-acyltransferase activator activity / Chylomicron clearance / positive regulation of phospholipid efflux / lipid transporter activity / Chylomicron remodeling / positive regulation of cholesterol metabolic process / regulation of amyloid fibril formation / regulation of behavioral fear response / cellular response to lipoprotein particle stimulus / positive regulation of low-density lipoprotein particle receptor catabolic process / regulation of protein metabolic process / Chylomicron assembly / high-density lipoprotein particle clearance / high-density lipoprotein particle remodeling / chylomicron / phospholipid efflux / AMPA glutamate receptor clustering / very-low-density lipoprotein particle receptor binding / lipoprotein catabolic process / multivesicular body, internal vesicle / melanosome organization / reverse cholesterol transport / high-density lipoprotein particle assembly / positive regulation of amyloid-beta clearance / positive regulation by host of viral process / : / low-density lipoprotein particle / protein import / lipoprotein biosynthetic process / cholesterol transfer activity / high-density lipoprotein particle / very-low-density lipoprotein particle / low-density lipoprotein particle remodeling / heparan sulfate proteoglycan binding / negative regulation of amyloid fibril formation / regulation of Cdc42 protein signal transduction / amyloid precursor protein metabolic process / triglyceride homeostasis / regulation of amyloid precursor protein catabolic process / cholesterol catabolic process / positive regulation of membrane protein ectodomain proteolysis / synaptic transmission, cholinergic / HDL remodeling / negative regulation of endothelial cell migration / cholesterol efflux / regulation of axon extension / artery morphogenesis / Scavenging by Class A Receptors / negative regulation of protein metabolic process / regulation of cholesterol metabolic process / positive regulation of dendritic spine development / triglyceride metabolic process / low-density lipoprotein particle receptor binding / positive regulation of amyloid fibril formation / regulation of innate immune response / virion assembly / negative regulation of amyloid-beta formation / locomotory exploration behavior / negative regulation of endothelial cell proliferation / antioxidant activity / positive regulation of endocytosis / lipoprotein particle binding / response to dietary excess / regulation of neuronal synaptic plasticity / negative regulation of blood vessel endothelial cell migration / negative regulation of long-term synaptic potentiation / negative regulation of platelet activation / positive regulation of cholesterol efflux / positive regulation of dendritic spine maintenance / negative regulation of blood coagulation / negative regulation of MAP kinase activity / long-term memory / negative regulation of protein secretion / intracellular transport / fatty acid homeostasis / regulation of protein-containing complex assembly / long-chain fatty acid transport / synaptic cleft

ドメイン・相同性:

Apolipoprotein A/E / : / Apolipoprotein A1/A4/E domain

構成要素:

Apolipoprotein E

• 生物種: Homo sapiens (ヒト)

登録者

• Lucas Kraft (University of Sussex, Brighton, UK)

モデル

試料

• 試料: 名称: Apolipoprotein E4 (methylated) bound to 37 µM Suramin; 試料濃度: 4 mg/ml / Entity id: 1109 / 1682
• バッファ: 名称: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

要素 #1109

名称: ApoE4 / タイプ: protein / 記述: Apolipoprotein E4 / 分子量: 34.712 / 分子数: 4 / 由来: Homo sapiens / 参照: UniProt: P02649
配列:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVRGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KRLAVYQAGA REGAERGLSA IRERLGPLVE QGRVRAATVG SLAGQPLQER AQAWGERLRA RMEEMGSRTR DRLDEVKEQV AEVRAKLEEQ AQQIRLQAEA FQARLKSWFE PLVEDMQRQW AGLVEKVQAA VGTSAAPVPS DNH

要素 #1682

タイプ: other / 記述: Suramin / 分子量: 1.297 / 分子数: 1
配列:

C51H40N6O2 3S6

実験情報

• ビーム: 設備名称: Diamond Light Source B21 / 地域: Didcot / 国: UK / 形状: 1 x 5 mm / 線源: X-ray synchrotron / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 4.036 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Apolipoprotein E4 (methylated) bound to 37 µM Suramin
測定日: 2018年5月3日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 28 / 単位: 1/A /

	Min	Max
Q	0.003	0.37

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 993 /

	Min	Max
Q	0.00989933	0.230678
P(R) point	1	993
R	0	196

結果

Experimental MW: 168 kDa / カーブのタイプ: single_conc / コメント: ApoE4 (methylated) + 37 µM Suramin

	P(R)	Guinier	Guinier error
前方散乱 I0	0.4441	0.44	0.0032
慣性半径, Rg	5.99 nm	5.808 nm	0.062

	Min	Max
D	-	19.6
Guinier point	32	85