SASDGS9: Truncated Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS)

Basic information

Entry

Database: SASBDB / ID: SASDGS9

Sample

Truncated Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS)

• Apolipoprotein E4 (1-191) (protein), ApoE4, Homo sapiens
• Suramin (other)

Function / homology

Function:

lipid transport involved in lipid storage / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / triglyceride-rich lipoprotein particle clearance / discoidal high-density lipoprotein particle / lipoprotein particle / negative regulation of triglyceride metabolic process / negative regulation of cholesterol biosynthetic process / regulation of amyloid-beta clearance / positive regulation of lipoprotein transport / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / chylomicron remnant clearance / chylomicron remnant / intermediate-density lipoprotein particle / maintenance of location in cell / very-low-density lipoprotein particle remodeling / acylglycerol homeostasis / NMDA glutamate receptor clustering / response to caloric restriction / phosphatidylcholine-sterol O-acyltransferase activator activity / Chylomicron clearance / positive regulation of phospholipid efflux / very-low-density lipoprotein particle clearance / Chylomicron remodeling / lipid transporter activity / positive regulation of cholesterol metabolic process / regulation of behavioral fear response / cellular response to lipoprotein particle stimulus / positive regulation of low-density lipoprotein particle receptor catabolic process / regulation of amyloid fibril formation / Chylomicron assembly / : / high-density lipoprotein particle clearance / chylomicron / phospholipid efflux / regulation of protein metabolic process / high-density lipoprotein particle remodeling / very-low-density lipoprotein particle receptor binding / AMPA glutamate receptor clustering / lipoprotein catabolic process / melanosome organization / multivesicular body, internal vesicle / reverse cholesterol transport / positive regulation of amyloid-beta clearance / positive regulation by host of viral process / high-density lipoprotein particle assembly / low-density lipoprotein particle / protein import / lipoprotein biosynthetic process / cholesterol transfer activity / high-density lipoprotein particle / very-low-density lipoprotein particle / cholesterol catabolic process / low-density lipoprotein particle remodeling / heparan sulfate proteoglycan binding / regulation of Cdc42 protein signal transduction / amyloid precursor protein metabolic process / negative regulation of amyloid fibril formation / triglyceride homeostasis / regulation of amyloid precursor protein catabolic process / positive regulation of membrane protein ectodomain proteolysis / synaptic transmission, cholinergic / HDL remodeling / negative regulation of endothelial cell migration / cholesterol efflux / regulation of axon extension / regulation of cholesterol metabolic process / negative regulation of protein metabolic process / artery morphogenesis / Scavenging by Class A Receptors / triglyceride metabolic process / positive regulation of dendritic spine development / low-density lipoprotein particle receptor binding / positive regulation of amyloid fibril formation / regulation of innate immune response / virion assembly / negative regulation of amyloid-beta formation / negative regulation of endothelial cell proliferation / locomotory exploration behavior / antioxidant activity / positive regulation of endocytosis / lipoprotein particle binding / response to dietary excess / negative regulation of blood vessel endothelial cell migration / regulation of neuronal synaptic plasticity / negative regulation of long-term synaptic potentiation / negative regulation of platelet activation / positive regulation of dendritic spine maintenance / negative regulation of blood coagulation / positive regulation of cholesterol efflux / negative regulation of MAP kinase activity / long-term memory / negative regulation of protein secretion / fatty acid homeostasis / regulation of protein-containing complex assembly / synaptic cleft / long-chain fatty acid transport / intracellular transport

Domain/homology:

Apolipoprotein A/E / : / Apolipoprotein A1/A4/E domain

Component:

Apolipoprotein E

• Biological species: Homo sapiens (human)

Contact author

• Lucas Kraft (University of Sussex, Brighton, UK)

Models

Sample

• Sample: Name: Truncated Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS); Specimen concentration: 10 mg/ml / Entity id: 1635 / 1682
• Buffer: Name: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

Entity #1635

Name: ApoE4 / Type: protein / Description: Apolipoprotein E4 (1-191) / Formula weight: 22.605 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P02649
Sequence:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVRGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KRLAVYQAGA REGAERGLSA IRERLGPLVE QGRVR

Entity #1682

Type: other / Description: Suramin / Formula weight: 1.297 / Num. of mol.: 1
Sequence:

C51H40N6O2 3S6

Experimental information

• Beam: Instrument name: Diamond Light Source B21 / City: Didcot / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.036 mm
• Detector: Name: Pilatus 2M

Scan

Title: Truncated Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS)
Measurement date: May 3, 2018 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 3 sec. / Number of frames: 17 / Unit: 1/A /

	Min	Max
Q	0.003	0.37

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 1447 /

	Min	Max
Q	0.0145731	0.336394
P(R) point	1	1447
R	0	83

Result

Experimental MW: 24 kDa / Type of curve: sec
Comments: Truncated ApoE4 (1-191) at 10 mg/mL was preequilibrated with 1 mM Suramin and then gel-filtered in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 8.0.

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.04744	0.047	5.6E-5
Radius of gyration, Rg	2.43 nm	2.374 nm	0.005

	Min	Max
D	-	8.3
Guinier point	53	233