SASDGR9: Truncated Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC...

Basic information

Entry

Database: SASBDB / ID: SASDGR9

Sample

Truncated Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC-SAXS)

• Heparin (other)
• Apolipoprotein E4 (1-191) (protein), ApoE4, Homo sapiens

Function / homology

Function:

positive regulation of heparan sulfate proteoglycan binding / lipid transport involved in lipid storage / chylomicron remnant / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / regulation of cellular response to very-low-density lipoprotein particle stimulus / positive regulation of low-density lipoprotein particle receptor catabolic process / metal chelating activity / triglyceride-rich lipoprotein particle clearance / lipoprotein particle / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / regulation of amyloid-beta clearance / negative regulation of cholesterol biosynthetic process / discoidal high-density lipoprotein particle / intermediate-density lipoprotein particle / chylomicron remnant clearance / maintenance of location in cell / very-low-density lipoprotein particle remodeling / very-low-density lipoprotein particle receptor binding / very-low-density lipoprotein particle clearance / Chylomicron clearance / NMDA glutamate receptor clustering / acylglycerol homeostasis / response to caloric restriction / Chylomicron remodeling / cellular response to lipoprotein particle stimulus / negative regulation of triglyceride metabolic process / phosphatidylcholine-sterol O-acyltransferase activator activity / positive regulation of phospholipid efflux / Chylomicron assembly / positive regulation of cholesterol metabolic process / regulation of amyloid fibril formation / regulation of behavioral fear response / regulation of protein metabolic process / high-density lipoprotein particle clearance / chylomicron / lipoprotein catabolic process / lipid transporter activity / high-density lipoprotein particle remodeling / phospholipid efflux / melanosome organization / multivesicular body, internal vesicle / AMPA glutamate receptor clustering / cholesterol transfer activity / reverse cholesterol transport / high-density lipoprotein particle assembly / positive regulation of amyloid-beta clearance / very-low-density lipoprotein particle / positive regulation by host of viral process / low-density lipoprotein particle / lipoprotein biosynthetic process / positive regulation of CoA-transferase activity / protein import / high-density lipoprotein particle / negative regulation of blood coagulation / low-density lipoprotein particle remodeling / negative regulation of amyloid fibril formation / heparan sulfate proteoglycan binding / synaptic transmission, cholinergic / regulation of Cdc42 protein signal transduction / amyloid precursor protein metabolic process / regulation of amyloid precursor protein catabolic process / cholesterol catabolic process / triglyceride homeostasis / positive regulation of membrane protein ectodomain proteolysis / negative regulation of protein metabolic process / HDL remodeling / triglyceride metabolic process / negative regulation of endothelial cell migration / Scavenging by Class A Receptors / cholesterol efflux / low-density lipoprotein particle receptor binding / artery morphogenesis / regulation of axon extension / regulation of cholesterol metabolic process / positive regulation of amyloid fibril formation / virion assembly / positive regulation of dendritic spine development / regulation of innate immune response / negative regulation of amyloid-beta formation / negative regulation of endothelial cell proliferation / locomotory exploration behavior / regulation of neuronal synaptic plasticity / antioxidant activity / lipoprotein particle binding / positive regulation of endocytosis / response to dietary excess / negative regulation of blood vessel endothelial cell migration / negative regulation of long-term synaptic potentiation / negative regulation of protein secretion / positive regulation of dendritic spine maintenance / negative regulation of platelet activation / positive regulation of cholesterol efflux / regulation of protein-containing complex assembly / intracellular transport / fatty acid homeostasis / long-term memory / long-chain fatty acid transport / positive regulation of lipid biosynthetic process / regulation of proteasomal protein catabolic process

Domain/homology:

Apolipoprotein A/E / : / Apolipoprotein A1/A4/E domain

Component:

Apolipoprotein E

• Biological species: Homo sapiens (human)

Contact author

• Lucas Kraft (University of Sussex, Brighton, UK)

Models

Sample

• Sample: Name: Truncated Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC-SAXS); Specimen concentration: 10 mg/ml / Entity id: 1633 / 1635
• Buffer: Name: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

Entity #1633

Type: other / Description: Heparin / Formula weight: 15 / Num. of mol.: 1
Sequence:

(C12H19NO20S3)n

Entity #1635

Name: ApoE4 / Type: protein / Description: Apolipoprotein E4 (1-191) / Formula weight: 22.605 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P02649
Sequence:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVRGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KRLAVYQAGA REGAERGLSA IRERLGPLVE QGRVR

Experimental information

• Beam: Instrument name: Diamond Light Source B21 / City: Didcot / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.036 mm
• Detector: Name: Pilatus 2M

Scan

Title: Truncated Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC-SAXS)
Measurement date: May 3, 2018 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 3 sec. / Number of frames: 11 / Unit: 1/A /

	Min	Max
Q	0.003	0.37

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 940 /

	Min	Max
Q	0.0163536	0.225337
P(R) point	1	940
R	0	168

Result

Experimental MW: 59 kDa / Type of curve: sec
Comments: Truncated ApoE4 (1-191) at 10 mg/mL was gel-filtered in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, 1 mg/mL heparin, pH 8.0.

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.06997	0.069	0.00045
Radius of gyration, Rg	4.51 nm	4.178 nm	0.041

	Min	Max
D	-	16.8
Guinier point	56	120