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- SASDGQ5: Vibrio cholerae hexameric DnaB helicase with bound ATP (VcDnaB.ATP) -

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Basic information

Entry
Database: SASBDB / ID: SASDGQ5
SampleVibrio cholerae hexameric DnaB helicase with bound ATP (VcDnaB.ATP)
  • Replicative DNA helicase (DnaB) (protein), DnaB.ATP, Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Function / homology
Function and homology information


primosome complex / DNA replication, synthesis of primer / DNA helicase activity / DNA helicase / hydrolase activity / DNA binding / ATP binding
Similarity search - Function
DNA helicase, DnaB type / DNA helicase, DnaB-like, N-terminal / DnaB-like helicase N terminal domain / DNA helicase, DnaB-like, N-terminal domain superfamily / DNA helicase DnaB, N-terminal/DNA primase DnaG, C-terminal / DnaB-like helicase C terminal domain / DNA helicase, DnaB-like, C-terminal / Superfamily 4 helicase domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Replicative DNA helicase
Similarity search - Component
Biological speciesVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (bacteria)
Contact author
  • Dominique DURAND (I2BC-CNRS, Paris, France)

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Structure visualization

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Models

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Sample

SampleName: Vibrio cholerae hexameric DnaB helicase with bound ATP (VcDnaB.ATP)
Specimen concentration: 28 mg/ml
BufferName: 20 mM Tris-HCl, 100 mM NaCl, 1 mM ATP / pH: 8.8
Entity #1898Name: DnaB.ATP / Type: protein / Description: Replicative DNA helicase (DnaB) / Formula weight: 52.781 / Num. of mol.: 6
Source: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
References: UniProt: A0A0H3AIB6
Sequence: MADPRTDNRN RKTSDAQVDA IKVPPHSLEA EQSVIGGLLL DNERWDTVSE HVVTQDFYSR PHRLIFDGVK SILEAGKPLD LITLSEYLEQ REQLEDVGGF AYLADLAKNT PSAANINAYA EIVAQRALVR NLIGVANEIA DAGYDPQGRN AEDLLDLAES KVFAIAEART ...Sequence:
MADPRTDNRN RKTSDAQVDA IKVPPHSLEA EQSVIGGLLL DNERWDTVSE HVVTQDFYSR PHRLIFDGVK SILEAGKPLD LITLSEYLEQ REQLEDVGGF AYLADLAKNT PSAANINAYA EIVAQRALVR NLIGVANEIA DAGYDPQGRN AEDLLDLAES KVFAIAEART SENEGPKNVD SILERTLERI ELLYKTPQDG VTGVNTGFTD LNKKTAGLQG SDLIIVAARP SMGKTTFAMN LCENAAMEQD KPVLIFSLEM PAEQIMMRML ASLSRVDQTK IRTGQLDDED WARISSTMGI LMEKKNMYID DSSGLTPTEV RSRARRIARE HGGLSLIMVD YLQLMRVPAL TDNRTLEIAE ISRSLKALAK ELNVPVVALS QLNRSLEQRA DKRPVNSDLR ESGSIEQDAD LIMFIYRDEV YHPDSALKGT AEIIIGKQRN GPIGSVRLTF QGQYSRFDNY AGPAFDDEHH HHHH

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Experimental information

BeamInstrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 1.8 mm
DetectorName: Eiger 4M / Pixsize x: 75 mm
Scan
Title: Vibrio cholerae hexameric DnaB helicase with bound ATP (VcDnaB.ATP)
Measurement date: Oct 4, 2018 / Storage temperature: 10 °C / Cell temperature: 15 °C / Exposure time: 2.99 sec. / Number of frames: 700 / Unit: 1/A /
MinMax
Q0.0061 0.4002
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 329 /
MinMax
Q0.00608132 0.172252
P(R) point1 329
R0 130
Result
Type of curve: sec
Comments: The scattered intensities were displayed on an absolute scale (cm-1) using the scattering of water. Frames were examined individually using the US-SOMO HPLC module and 10 identical frames ...Comments: The scattered intensities were displayed on an absolute scale (cm-1) using the scattering of water. Frames were examined individually using the US-SOMO HPLC module and 10 identical frames obtained around the maximum of the elution peak were averaged and further processed.
ExperimentalPorod
MW328 kDa365 kDa
Volume-585 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.2801 0.001 0.278 0.001
Radius of gyration, Rg4.67 nm0.02 4.64 nm0.05

MinMaxError
D-13 1
Guinier point1 32 -

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