SASDGN8: Apolipoprotein E4 (K143A K146A) mutant bound to 37 µM Suramin

基本情報

登録情報

データベース: SASBDB / ID: SASDGN8

試料

Apolipoprotein E4 (K143A K146A) mutant bound to 37 µM Suramin

• Apolipoprotein E4 (K143A K146A) mutant (protein), ApoE4 (K143A K146A), Homo sapiens
• Suraminスラミン (other)

機能・相同性

分子機能:

chylomicron remnant / lipid transport involved in lipid storage / triglyceride-rich lipoprotein particle clearance / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / positive regulation of heparan sulfate proteoglycan binding / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / discoidal high-density lipoprotein particle / chylomicron remnant clearance / リポタンパク質 / maintenance of location in cell / very-low-density lipoprotein particle clearance / intermediate-density lipoprotein particle / very-low-density lipoprotein particle remodeling / regulation of amyloid-beta clearance / response to caloric restriction / Chylomicron clearance / negative regulation of triglyceride metabolic process / NMDA glutamate receptor clustering / Chylomicron remodeling / phosphatidylcholine-sterol O-acyltransferase activator activity / lipid transporter activity / positive regulation of phospholipid efflux / カイロミクロン / Chylomicron assembly / positive regulation of low-density lipoprotein particle receptor catabolic process / positive regulation of cholesterol metabolic process / regulation of behavioral fear response / regulation of protein metabolic process / high-density lipoprotein particle clearance / multivesicular body, internal vesicle / lipoprotein catabolic process / very-low-density lipoprotein particle receptor binding / regulation of amyloid fibril formation / melanosome organization / high-density lipoprotein particle remodeling / phospholipid efflux / low-density lipoprotein particle / AMPA glutamate receptor clustering / cholesterol transfer activity / positive regulation by host of viral process / reverse cholesterol transport / positive regulation of amyloid-beta clearance / high-density lipoprotein particle assembly / very-low-density lipoprotein particle / positive regulation of CoA-transferase activity / lipoprotein biosynthetic process / protein import / negative regulation of blood coagulation / high-density lipoprotein particle / low-density lipoprotein particle remodeling / negative regulation of amyloid fibril formation / synaptic transmission, cholinergic / negative regulation of cholesterol biosynthetic process / heparan sulfate proteoglycan binding / regulation of Cdc42 protein signal transduction / amyloid precursor protein metabolic process / regulation of amyloid precursor protein catabolic process / triglyceride homeostasis / positive regulation of membrane protein ectodomain proteolysis / HDL remodeling / negative regulation of endothelial cell migration / Scavenging by Class A Receptors / cholesterol efflux / negative regulation of protein metabolic process / artery morphogenesis / regulation of axon extension / regulation of cholesterol metabolic process / low-density lipoprotein particle receptor binding / positive regulation of amyloid fibril formation / triglyceride metabolic process / positive regulation of dendritic spine development / virion assembly / regulation of innate immune response / regulation of neuronal synaptic plasticity / locomotory exploration behavior / negative regulation of amyloid-beta formation / lipoprotein particle binding / negative regulation of endothelial cell proliferation / positive regulation of endocytosis / antioxidant activity / cGMP-mediated signaling / cholesterol catabolic process / response to dietary excess / negative regulation of blood vessel endothelial cell migration / negative regulation of long-term synaptic potentiation / positive regulation of dendritic spine maintenance / negative regulation of platelet activation / positive regulation of cholesterol efflux / intracellular transport / regulation of protein-containing complex assembly / negative regulation of protein secretion / fatty acid homeostasis / long-term memory / long-chain fatty acid transport / positive regulation of lipid biosynthetic process / synaptic cleft / regulation of proteasomal protein catabolic process

ドメイン・相同性:

Apolipoprotein A/E / Apolipoprotein A1/A4/E domain

構成要素:

アポリポプロテインE

• 生物種: Homo sapiens (ヒト)

登録者

• Lucas Kraft (University of Sussex, Brighton, UK)

モデル

試料

• 試料: 名称: Apolipoprotein E4 (K143A K146A) mutant bound to 37 µM Suramin; 試料濃度: 4 mg/ml / Entity id: 1634 / 1682
• バッファ: 名称: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

要素 #1634

名称: ApoE4 (K143A K146A) / タイプ: protein / 記述: Apolipoprotein E4 (K143A K146A) mutant / 分子量: 34.598 / 分子数: 4 / 由来: Homo sapiens / 参照: UniProt: P02649
配列:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVRGRLV QYRGEVQAML GQSTEELRVR LASHLRALRA RLLRDADDLQ KRLAVYQAGA REGAERGLSA IRERLGPLVE QGRVRAATVG SLAGQPLQER AQAWGERLRA RMEEMGSRTR DRLDEVKEQV AEVRAKLEEQ AQQIRLQAEA FQARLKSWFE PLVEDMQRQW AGLVEKVQAA VGTSAAPVPS DNH

要素 #1682

タイプ: other / 記述: Suraminスラミン / 分子量: 1.297 / 分子数: 1
配列:

C51H40N6O2 3S6

実験情報

• ビーム: 設備名称: Diamond Light Source B21 / 地域: Didcot / 国: UK / 形状: 1 x 5 mm / 線源: X-ray synchrotronシンクロトロン / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 4.036 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Apolipoprotein E4 (K143A K146A) mutant bound to 37 µM Suramin
測定日: 2018年7月21日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 28 / 単位: 1/A /

	Min	Max
Q	0.0035	0.3696

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 1001 /

	Min	Max
Q	0.0101798	0.232841
P(R) point	1	1001
R	0	199

結果

Experimental MW: 150 kDa / カーブのタイプ: single_conc / コメント: ApoE4 (K143A K146A) + 37 µM Suramin

	P(R)	Guinier	Guinier error
前方散乱 I0	0.3592	0.36	0.00065
慣性半径, Rg	5.99 nm	5.939 nm	0.018

	Min	Max
D	-	19.9
Guinier point	31	73