SASDGL8: Apolipoprotein E4 (K143A K146A) mutant bound to 330 µM Suramin

基本情報

登録情報

データベース: SASBDB / ID: SASDGL8

試料

Apolipoprotein E4 (K143A K146A) mutant bound to 330 µM Suramin

• Apolipoprotein E4 (K143A K146A) mutant (protein), ApoE4 (K143A K146A), Homo sapiens
• Suramin (other)

機能・相同性

分子機能:

lipid transport involved in lipid storage / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / triglyceride-rich lipoprotein particle clearance / discoidal high-density lipoprotein particle / lipoprotein particle / negative regulation of triglyceride metabolic process / maintenance of location in cell / regulation of amyloid-beta clearance / negative regulation of cholesterol biosynthetic process / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / chylomicron remnant clearance / positive regulation of lipoprotein transport / chylomicron remnant / intermediate-density lipoprotein particle / acylglycerol homeostasis / NMDA glutamate receptor clustering / very-low-density lipoprotein particle remodeling / Chylomicron clearance / phosphatidylcholine-sterol O-acyltransferase activator activity / positive regulation of phospholipid efflux / Chylomicron remodeling / lipid transporter activity / positive regulation of low-density lipoprotein particle receptor catabolic process / cellular response to lipoprotein particle stimulus / regulation of protein metabolic process / Chylomicron assembly / very-low-density lipoprotein particle clearance / high-density lipoprotein particle clearance / phospholipid efflux / chylomicron / response to caloric restriction / very-low-density lipoprotein particle receptor binding / lipoprotein catabolic process / high-density lipoprotein particle remodeling / positive regulation of cholesterol metabolic process / melanosome organization / regulation of amyloid fibril formation / regulation of behavioral fear response / multivesicular body, internal vesicle / reverse cholesterol transport / positive regulation of amyloid-beta clearance / high-density lipoprotein particle assembly / host-mediated activation of viral process / low-density lipoprotein particle / lipoprotein biosynthetic process / cholesterol transfer activity / high-density lipoprotein particle / protein import / cholesterol catabolic process / very-low-density lipoprotein particle / heparan sulfate proteoglycan binding / low-density lipoprotein particle remodeling / amyloid precursor protein metabolic process / negative regulation of amyloid fibril formation / regulation of Cdc42 protein signal transduction / synaptic transmission, cholinergic / positive regulation of membrane protein ectodomain proteolysis / HDL remodeling / regulation of amyloid precursor protein catabolic process / negative regulation of endothelial cell migration / regulation of cholesterol metabolic process / cholesterol efflux / artery morphogenesis / negative regulation of protein metabolic process / regulation of axon extension / Scavenging by Class A Receptors / triglyceride homeostasis / triglyceride metabolic process / low-density lipoprotein particle receptor binding / positive regulation of amyloid fibril formation / virion assembly / regulation of innate immune response / negative regulation of endothelial cell proliferation / positive regulation of dendritic spine development / negative regulation of MAP kinase activity / response to dietary excess / antioxidant activity / lipoprotein particle binding / negative regulation of amyloid-beta formation / AMPA glutamate receptor clustering / locomotory exploration behavior / negative regulation of long-term synaptic potentiation / negative regulation of blood vessel endothelial cell migration / negative regulation of platelet activation / negative regulation of blood coagulation / positive regulation of dendritic spine maintenance / positive regulation of endocytosis / regulation of neuronal synaptic plasticity / positive regulation of cholesterol efflux / negative regulation of protein secretion / fatty acid homeostasis / regulation of protein-containing complex assembly / long-term memory / long-chain fatty acid transport / intracellular transport / synaptic cleft / Nuclear signaling by ERBB4

ドメイン・相同性:

Apolipoprotein A/E / : / Apolipoprotein A1/A4/E domain

構成要素:

Apolipoprotein E

• 生物種: Homo sapiens (ヒト)

登録者

• Lucas Kraft (University of Sussex, Brighton, UK)

モデル

試料

• 試料: 名称: Apolipoprotein E4 (K143A K146A) mutant bound to 330 µM Suramin; 試料濃度: 4 mg/ml / Entity id: 1634 / 1682
• バッファ: 名称: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

要素 #1634

名称: ApoE4 (K143A K146A) / タイプ: protein / 記述: Apolipoprotein E4 (K143A K146A) mutant / 分子量: 34.598 / 分子数: 4 / 由来: Homo sapiens / 参照: UniProt: P02649
配列:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVRGRLV QYRGEVQAML GQSTEELRVR LASHLRALRA RLLRDADDLQ KRLAVYQAGA REGAERGLSA IRERLGPLVE QGRVRAATVG SLAGQPLQER AQAWGERLRA RMEEMGSRTR DRLDEVKEQV AEVRAKLEEQ AQQIRLQAEA FQARLKSWFE PLVEDMQRQW AGLVEKVQAA VGTSAAPVPS DNH

要素 #1682

タイプ: other / 記述: Suramin / 分子量: 1.297 / 分子数: 1
配列:

C51H40N6O2 3S6

実験情報

• ビーム: 設備名称: Diamond Light Source B21 / 地域: Didcot / 国: UK / 形状: 1 x 5 mm / 線源: X-ray synchrotron / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 4.036 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Apolipoprotein E4 (K143A K146A) mutant bound to 330 µM Suramin
測定日: 2018年7月21日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 28 / 単位: 1/A /

	Min	Max
Q	0.0035	0.37

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 927 /

	Min	Max
Q	0.0110705	0.217255
P(R) point	1	927
R	0	224

結果

Experimental MW: 201 kDa / カーブのタイプ: single_conc / コメント: ApoE4 (K143A K146A) + 330 µM Suramin

	P(R)	Guinier	Guinier error
前方散乱 I0	0.5277	0.52	0.004
慣性半径, Rg	6.27 nm	5.974 nm	0.067

	Min	Max
D	-	22.4
Guinier point	34	78