[English] 日本語
![](img/lk-miru.gif)
- SASDGC3: DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 5.5) -
+
Open data
-
Basic information
Entry | ![]() |
---|---|
![]() | DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 5.5)
|
Function / homology | ![]() HU-DNA complex / DnaA-HU complex / bacterial nucleoid packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding ...HU-DNA complex / DnaA-HU complex / bacterial nucleoid packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding / identical protein binding / membrane / cytosol Similarity search - Function |
Biological species | ![]() ![]() ![]() ![]() |
![]() |
|
-
Structure visualization
-
Downloads & links
-Data source
SASBDB page | ![]() |
---|
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
---|
-Models
-
Sample
![]() | Name: DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 5.5) Entity id: 1622 / 1623 / 1632 |
---|---|
Buffer | Name: 10 mM Bis-Tris, 50 mM NaCl / pH: 5.5 |
Entity #1622 | Type: DNA / Description: 80bp_DNA Forward / Formula weight: 24.849 / Num. of mol.: 1 / Source: Escherichia coli Sequence: AATGAGGTAA CAACGAAAGC AGATGATAGC TGCTTATCAA TTTGTTGCAA ACAAGTAGCC GCGCCCAATG AGGTAACAAT |
Entity #1623 | Type: DNA / Description: 80bp_DNA Reverse / Formula weight: 24.612 / Num. of mol.: 1 / Source: Escherichia coli K-12 Sequence: TTACTCCATT GTTGCTTTCG TCTACTATCG ACGAATAGTT AAACAACGTT TGTTCATCGG CGCGGGTTAC TCCATTGTTA |
Entity #1632 | Type: protein / Description: DNA-binding protein HU-alpha, E34K / Formula weight: 9.534 / Num. of mol.: 2 / Source: Escherichia coli K-12 / References: UniProt: P0ACF0 Sequence: MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITKSLKEGD AVQLVGFGTF KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK |
-Experimental information
Beam | Instrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS) City: Berkeley, CA / 国: USA ![]() | ||||||
---|---|---|---|---|---|---|---|
Detector | Name: Pilatus3 X 2M / Pixsize x: 172 mm | ||||||
Scan | Measurement date: Nov 2, 2018 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 3 sec. / Number of frames: 300 / Unit: 1/A /
| ||||||
Result | Experimental MW: 69 kDa / Rg from PR: 2 nm / Type of curve: single_conc Comments: his curve is part of a pH series. Increasing the pH removes aggregation presumably due to protein surface charge changes. The SAXS data for this entry is measured from aggregated material ...Comments: his curve is part of a pH series. Increasing the pH removes aggregation presumably due to protein surface charge changes. The SAXS data for this entry is measured from aggregated material and therefore the Rg and I(0) parameters do not apply. |