SASDGB3: DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 4.5)

Basic information

Entry

Database: SASBDB / ID: SASDGB3

Sample

DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 4.5)

• 80bp_DNA Forward (DNA), Escherichia coli
• 80bp_DNA Reverse (DNA), Escherichia coli K-12
• DNA-binding protein HU-alpha, E34K (protein), Escherichia coli K-12

Function / homology

Function:

HU-DNA complex / DnaA-HU complex / bacterial nucleoid DNA packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding / identical protein binding / membrane / cytosol

Domain/homology:

Histone-like DNA-binding protein, conserved site / Bacterial histone-like DNA-binding proteins signature. / Histone-like DNA-binding protein / Bacterial DNA-binding protein / bacterial (prokaryotic) histone like domain / Integration host factor (IHF)-like DNA-binding domain superfamily

Component:

DNA-binding protein HU-alpha

• Biological species: Escherichia coli (E. coli); Escherichia coli K-12 (bacteria)

Contact author

• Soumya G Remesh (Lawrence Berkeley National laboratory, Berkeley, CA, USA)

Models

Sample

• Sample: Name: DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 4.5); Entity id: 1622 / 1623 / 1632
• Buffer: Name: 10 mM sodium acetate, 50 mM NaCl / pH: 4.5

Entity #1622

Type: DNA / Description: 80bp_DNA Forward / Formula weight: 24.849 / Num. of mol.: 1 / Source: Escherichia coli
Sequence:

AATGAGGTAA CAACGAAAGC AGATGATAGC TGCTTATCAA TTTGTTGCAA ACAAGTAGCC GCGCCCAATG AGGTAACAAT

Entity #1623

Type: DNA / Description: 80bp_DNA Reverse / Formula weight: 24.612 / Num. of mol.: 1 / Source: Escherichia coli K-12
Sequence:

TTACTCCATT GTTGCTTTCG TCTACTATCG ACGAATAGTT AAACAACGTT TGTTCATCGG CGCGGGTTAC TCCATTGTTA

Entity #1632

Type: protein / Description: DNA-binding protein HU-alpha, E34K / Formula weight: 9.534 / Num. of mol.: 2 / Source: Escherichia coli K-12 / References: UniProt: P0ACF0
Sequence:

MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITKSLKEGD AVQLVGFGTF KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK

Experimental information

• Beam: Instrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS); City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 1.5 mm
• Detector: Name: Pilatus3 X 2M / Pixsize x: 172 mm

Scan

Title: DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 4.5)
Measurement date: Nov 2, 2018 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 3 sec. / Number of frames: 300 / Unit: 1/A /

	Min	Max
Q	0.0081	0.391

• Result: Experimental MW: 65 kDa / Rg from PR: 2 nm / Type of curve: single_conc; Comments: This curve is part of a pH series. Increasing the pH removes aggregation presumably due to protein surface charge changes. The SAXS data for this entry is measured from aggregated material and therefore the Rg and I(0) parameters do not apply.