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- SASDGA7: Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN... -

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Basic information

Entry
Database: SASBDB / ID: SASDGA7
SampleTrypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 7-15
  • Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN (7-15) (protein), tbMORNdelta6, Trypanosoma brucei
Function / homologybilobe structure / Possible plasma membrane-binding motif in junctophilins, PIP-5-kinases and protein kinases. / MORN motif / MORN repeat / endocytosis / cytosol / MORN repeat-containing protein 1
Function and homology information
Biological speciesTrypanosoma brucei (eukaryote)
Contact author
  • Melissa Graewert (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #2868
Type: atomic / Chi-square value: 1.160 / P-value: 0.032524

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Sample

SampleName: Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 7-15
Specimen concentration: 3.8 mg/ml
BufferName: 20 mM Tris-HCl, 200 mM NaCl, 2% (w/v) glycerol, 0.5 mM DTT
pH: 8.5 / Comment: SEC-SAXS buffer
Entity #1920Name: tbMORNdelta6 / Type: protein
Description: Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN (7-15)
Formula weight: 25.927 / Num. of mol.: 2 / Source: Trypanosoma brucei / References: UniProt: Q587D3
Sequence: MSEKYDGEWN EGRMQGWGKY FYADGGVYEG EWVDGRMHGR GTYVFPNGNK YEGEWVEDRK DGYGILLYTN GERYEGYWHL DKAHGKGTLT FLQGDRYVGE WHYGKKHGHG VLSYSNGDTY DGEWRDDDAW GYGVLQYANG CRYEGEWAED RRHGKGLLVL PDGSSYEGSF ...Sequence:
MSEKYDGEWN EGRMQGWGKY FYADGGVYEG EWVDGRMHGR GTYVFPNGNK YEGEWVEDRK DGYGILLYTN GERYEGYWHL DKAHGKGTLT FLQGDRYVGE WHYGKKHGHG VLSYSNGDTY DGEWRDDDAW GYGVLQYANG CRYEGEWAED RRHGKGLLVL PDGSSYEGSF AHGKKDGPGK IILKDGSMYI GTWKDGVIVG QGEFRLSENC DLSNPDYSAW SHPQFEK

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3 mm
DetectorName: Pilatus 2M
Scan
Title: Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 7-15
Measurement date: Jun 11, 2016 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 60 / Unit: 1/nm /
MinMax
Q0.0796 3.0522
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 690 /
MinMax
Q0.0822875 1.9596
P(R) point1 690
R0 15.5
Result
Type of curve: sec
ExperimentalStandard errorPorod
MW46 kDa1 35 kDa
Volume--56 nm3

P(R)GuinierGuinier error
Forward scattering, I02876 2829 6
Radius of gyration, Rg4.379 nm4.08 nm-

MinMax
D-15.5
Guinier point2 88

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