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- SASDGA5: The C-terminal cell-surface signaling domain of the Pseudomonas c... -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDGA5
SampleThe C-terminal cell-surface signaling domain of the Pseudomonas capeferrum anti-sigma regulator PupR
  • PupR protein (protein), PupR, Pseudomonas putida
Function / homologyFecR, N-terminal / Domain of unknown function (DUF4880) / FecR protein / Fe(2+)-dicitrate sensor, transmembrane component / FecR protein / sigma factor antagonist activity / PupR protein
Function and homology information
Biological speciesPseudomonas putida (bacteria)
Contact author
  • Jaime Jensen (Vanderbilt University)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3780
Type: atomic / Chi-square value: 1.54729964997 / P-value: 0.000262
Model #3782
Type: atomic / Chi-square value: 4.862
Model #3785
Type: dummy / Software: (DAMFILT 5.0 (r4547)) / Radius of dummy atoms: 2.00 A / Chi-square value: 1.456 / P-value: 0.998201

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Sample

SampleName: The C-terminal cell-surface signaling domain of the Pseudomonas capeferrum anti-sigma regulator PupR
Specimen concentration: 9.6 mg/ml
BufferName: 25 mM HEPES 400 mM LiCl 10% v/v glycerol / pH: 7.5
Entity #1893Name: PupR / Type: protein / Description: PupR protein / Formula weight: 24.124 / Num. of mol.: 1 / Source: Pseudomonas putida / References: UniProt: Q52209
Sequence: GAMGQDWRAD YHSRIGEQRR LTLADGTQVQ LNTDSALNVA FDQQARRLRL VRGEMLITRP ALADSRPLWV DTEHGRLEST LAQFNVRLHG QHTQATVYQG SVALQPALHA YPPILLGAGE QASFNQQGLL ARQAVAAVAP AWSQGMLVAQ GQPLAAFIED LARYRRGHLA ...Sequence:
GAMGQDWRAD YHSRIGEQRR LTLADGTQVQ LNTDSALNVA FDQQARRLRL VRGEMLITRP ALADSRPLWV DTEHGRLEST LAQFNVRLHG QHTQATVYQG SVALQPALHA YPPILLGAGE QASFNQQGLL ARQAVAAVAP AWSQGMLVAQ GQPLAAFIED LARYRRGHLA CDPALAGLRV SGTFPLENTD KIIAAVAETL QLEVQHFTRY WVTLKPRMA

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Experimental information

BeamInstrument name: Advanced Photon Source (APS), Argonne National Laboratory BioCAT 18ID
City: Lemont, IL / : USA / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 3.5 mm
DetectorName: Pilatus3 X 1M / Pixsize x: 0.172 mm
Scan
Title: The C-terminal cell-surface signaling domain of the Pseudomonas capeferrum anti-sigma regulator PupR
Measurement date: Mar 16, 2016 / Cell temperature: 25 °C / Unit: 1/A /
MinMax
Q0.0262 0.3665
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 972 /
MinMax
Q0.0344232 0.330218
P(R) point1 972
R0 75
Result
Type of curve: sec /
ExperimentalPorod
MW26 kDa29 kDa
Volume-48.6 nm3

P(R)GuinierGuinier error
Forward scattering, I017.45 17.6 0.05
Radius of gyration, Rg2.23 nm2.23 nm0.1

MinMax
D-7.5
Guinier point1 106

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