SASDG98: Apolipoprotein E2 bound to 4 µM Suramin (Apolipoprotein E2, ApoE2...

基本情報

登録情報

データベース: SASBDB / ID: SASDG98

試料

Apolipoprotein E2 bound to 4 µM Suramin

• Apolipoprotein E2 (protein), ApoE2, Homo sapiens
• Suramin (other)

機能・相同性

分子機能:

lipid transport involved in lipid storage / maintenance of location in cell / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / triglyceride-rich lipoprotein particle clearance / negative regulation of triglyceride metabolic process / discoidal high-density lipoprotein particle / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / chylomicron remnant clearance / negative regulation of cholesterol biosynthetic process / chylomicron remnant / lipoprotein particle / regulation of amyloid-beta clearance / intermediate-density lipoprotein particle / NMDA glutamate receptor clustering / very-low-density lipoprotein particle remodeling / Chylomicron clearance / acylglycerol homeostasis / phosphatidylcholine-sterol O-acyltransferase activator activity / positive regulation of phospholipid efflux / lipid carrier activity / positive regulation of low-density lipoprotein particle receptor catabolic process / Chylomicron remodeling / cellular response to lipoprotein particle stimulus / very-low-density lipoprotein particle clearance / Chylomicron assembly / high-density lipoprotein particle clearance / phospholipid efflux / lipoprotein catabolic process / very-low-density lipoprotein particle receptor binding / regulation of protein metabolic process / chylomicron / high-density lipoprotein particle remodeling / multivesicular body, internal vesicle / positive regulation of amyloid-beta clearance / reverse cholesterol transport / positive regulation of cholesterol metabolic process / regulation of amyloid fibril formation / high-density lipoprotein particle assembly / host-mediated activation of viral process / lipoprotein biosynthetic process / melanosome organization / cholesterol transfer activity / regulation of behavioral fear response / low-density lipoprotein particle / cholesterol catabolic process / protein import / high-density lipoprotein particle / very-low-density lipoprotein particle / low-density lipoprotein particle remodeling / amyloid precursor protein metabolic process / response to caloric restriction / heparan sulfate proteoglycan binding / negative regulation of amyloid fibril formation / synaptic transmission, cholinergic / positive regulation of membrane protein ectodomain proteolysis / regulation of amyloid precursor protein catabolic process / regulation of Cdc42 protein signal transduction / negative regulation of endothelial cell migration / HDL remodeling / artery morphogenesis / negative regulation of protein metabolic process / regulation of cholesterol metabolic process / cholesterol efflux / regulation of axon extension / Scavenging by Class A Receptors / triglyceride metabolic process / triglyceride homeostasis / low-density lipoprotein particle receptor binding / positive regulation of amyloid fibril formation / virion assembly / regulation of innate immune response / negative regulation of endothelial cell proliferation / negative regulation of platelet-derived growth factor receptor signaling pathway / antioxidant activity / positive regulation of lipoprotein transport / response to dietary excess / positive regulation of dendritic spine development / negative regulation of amyloid-beta formation / lipoprotein particle binding / AMPA glutamate receptor clustering / negative regulation of blood vessel endothelial cell migration / negative regulation of long-term synaptic potentiation / negative regulation of platelet activation / locomotory exploration behavior / negative regulation of blood coagulation / positive regulation of dendritic spine maintenance / negative regulation of protein secretion / positive regulation of cholesterol efflux / fatty acid homeostasis / intracellular transport / regulation of neuronal synaptic plasticity / regulation of protein-containing complex assembly / positive regulation of endocytosis / long-chain fatty acid transport / Nuclear signaling by ERBB4 / positive regulation of lipid biosynthetic process / long-term memory

ドメイン・相同性:

Apolipoprotein A/E / : / Apolipoprotein A1/A4/E domain

構成要素:

Apolipoprotein E

• 生物種: Homo sapiens (ヒト)

登録者

• Lucas Kraft (University of Sussex, Brighton, UK)

モデル

試料

• 試料: 名称: Apolipoprotein E2 bound to 4 µM Suramin / 試料濃度: 4 mg/ml / Entity id: 1086 / 1682
• バッファ: 名称: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

要素 #1086

名称: ApoE2 / タイプ: protein / 記述: Apolipoprotein E2 / 分子量: 34.606 / 分子数: 4 / 由来: Homo sapiens / 参照: UniProt: P02649
配列:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVCGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KCLAVYQAGA REGAERGLSA IRERLGPLVE QGRVRAATVG SLAGQPLQER AQAWGERLRA RMEEMGSRTR DRLDEVKEQV AEVRAKLEEQ AQQIRLQAEA FQARLKSWFE PLVEDMQRQW AGLVEKVQAA VGTSAAPVPS DNH

要素 #1682

タイプ: other / 記述: Suramin / 分子量: 1.297 / 分子数: 1
配列:

C51H40N6O2 3S6

実験情報

• ビーム: 設備名称: Diamond Light Source B21 / 地域: Didcot / 国: UK / 形状: 1 x 5 mm / 線源: X-ray synchrotron / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 4.036 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Apolipoprotein E2 bound to 4 µM Suramin / 測定日: 2018年5月3日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 28 / 単位: 1/A /

	Min	Max
Q	0.003	0.37

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 951 /

	Min	Max
Q	0.010567	0.221998
P(R) point	1	951
R	0	205

結果

Experimental MW: 175 kDa / カーブのタイプ: single_conc / コメント: ApoE2 + 4 µM Suramin

	P(R)	Guinier	Guinier error
前方散乱 I0	0.4693	0.47	0.0042
慣性半径, Rg	6.19 nm	6.088 nm	0.08

	Min	Max
D	-	20.5
Guinier point	36	79