SASDG95: Phosphorylated resistance to inhibitors of cholinesterase 8 homol...

Basic information

• Entry: Database: SASBDB / ID: SASDG95

Sample

Phosphorylated resistance to inhibitors of cholinesterase 8 homolog A (Ric-8A, 1-491) and G protein complex

• Resistance to inhibitors of cholinesterase 8 homolog A (protein), Ric-8A, Rattus norvegicus
• Guanine nucleotide-binding protein G(i) subunit alpha-1 (protein), Gia, Rattus norvegicus

Function / homology

Function:

cell-cell adhesion involved in gastrulation / adenylate cyclase regulator activity / Extra-nuclear estrogen signaling / Adenylate cyclase inhibitory pathway / cell migration involved in gastrulation / basement membrane organization / vasculature development / negative regulation of synaptic transmission / Adrenaline,noradrenaline inhibits insulin secretion / ADP signalling through P2Y purinoceptor 12 / GTPase activating protein binding / G alpha (i) signalling events / neurotransmitter receptor localization to postsynaptic specialization membrane / response to light stimulus / G-protein alpha-subunit binding / adenylate cyclase inhibitor activity / positive regulation of protein localization to cell cortex / T cell migration / gastrulation / protein folding chaperone / D2 dopamine receptor binding / adenylate cyclase-inhibiting serotonin receptor signaling pathway / G protein-coupled serotonin receptor binding / guanyl-nucleotide exchange factor activity / cellular response to forskolin / regulation of mitotic spindle organization / GTPase activator activity / chemokine-mediated signaling pathway / response to prostaglandin E / positive regulation of cholesterol biosynthetic process / negative regulation of insulin secretion / visual learning / G protein-coupled receptor binding / centriolar satellite / G-protein beta/gamma-subunit complex binding / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway / GDP binding / heterotrimeric G-protein complex / G protein activity / midbody / cell cortex / in utero embryonic development / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / postsynapse / ciliary basal body / G protein-coupled receptor signaling pathway / cell division / GTPase activity / centrosome / GTP binding / nucleolus / glutamatergic synapse / magnesium ion binding / Golgi apparatus / protein-containing complex / nucleoplasm / membrane / plasma membrane / cytoplasm / cytosol

Domain/homology:

Synembryn / Guanine nucleotide exchange factor, Ric8 / Guanine nucleotide exchange factor synembryn / G-protein alpha subunit, group I / G protein alpha subunit, helical insertion / G protein alpha subunit / Guanine nucleotide binding protein (G-protein), alpha subunit / G-protein alpha subunit / G-alpha domain profile. / Armadillo-like helical / Armadillo-type fold / P-loop containing nucleoside triphosphate hydrolase

Component:

Chaperone Ric-8A / Guanine nucleotide-binding protein G(i) subunit alpha-1

• Biological species: Rattus norvegicus (Norway rat)

Contact author

• Tung-Chung Mou (University of Montana, Montana, USA)

Models

• Model #3779: ; Type: dummy / Radius of dummy atoms: 3.25 A / Chi-square value: 0.910 / P-value: 0.257845

Sample

• Sample: Name: Phosphorylated resistance to inhibitors of cholinesterase 8 homolog A (Ric-8A, 1-491) and G protein complex; Specimen concentration: 5 mg/ml / Entity id: 1891 / 1892
• Buffer: Name: 25 mM HEPES, 150 mM NaCl / pH: 8

Entity #1891

Name: Ric-8A / Type: protein
Description: Resistance to inhibitors of cholinesterase 8 homolog A
Formula weight: 55.559 / Num. of mol.: 1 / Source: Rattus norvegicus / References: UniProt: B1H241
Sequence:

MEPRAVADAL ETGEEDAVTE ALRSFNREHS QSFTFDDAQQ EDRKRLAKLL VSVLEQGLSP KHRVTWLQTI RILSRDRSCL DSFASRQSLH ALACYADIAI SEEPIPQPPD MDVLLESLKC LCNLVLSSPT AQMLAAEARL VVRLAERVGL YRKRSYPHEV QFFDLRLLFL LTALRTDVRQ QLFQELHGVR LLTDALELTL GVAPKENPLV ILPAQETERA MEILKVLFNI TYDSVKREVD EEDAALYRYL GTLLRHCVMA DAAGDRTEEF HGHTVNLLGN LPLKCLDVLL ALELHEGSLE FMGVNMDVIN ALLAFLEKRL HQTHRLKECV APVLSVLTEC ARMHRPARKF LKAQVLPPLR DVRTRPEVGD LLRNKLVRLM THLDTDVKRV AAEFLFVLCS ESVPRFIKYT GYGNAAGLLA ARGLMAGGRP EGQYSEDEDT DTEEYREAKA SINPVTGRVE EKPPNPMEGM TEEQKEHEAM KLVNMFDKLS R

Entity #1892

Name: Gia / Type: protein
Description: Guanine nucleotide-binding protein G(i) subunit alpha-1
Formula weight: 37.824 / Num. of mol.: 1 / Source: Rattus norvegicus / References: UniProt: P10824
Sequence:

REDGEKAARE VKLLLLGAGE SGKSTIVKQM KIIHEAGYSE EECKQYKAVV YSNTIQSIIA IIRAMGRLKI DFGDAARADD ARQLFVLAGA AEEGFMTAEL AGVIKRLWKD SGVQACFNRS REYQLNDSAA YYLNDLDRIA QPNYIPTQQD VLRTRVKTTG IVETHFTFKD LHFKMFDVGG QRSERKKWIH CFEGVTAIIF CVALSDYDLV LAEDEEMNRM HESMKLFDSI CNNKWFTDTS IILFLNKKDL FEEKIKKSPL TICYPEYAGS NTYEEAAAYI QCQFEDLNKR KDTKEIYTHF TCATDTKNVQ FVFDAVTDVI IKNNLKDCGL F

Experimental information

• Beam: Instrument name: Advanced Photon Source (APS), Argonne National Laboratory BioCAT 18ID; City: Lemont, IL / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 3.67 mm
• Detector: Name: Pilatus3 X 1M / Pixsize x: 0.172 mm

Scan

Title: Phosphorylated resistance to inhibitors of cholinesterase 8 homolog A (Ric-8A, 1-491) and G protein complex
Measurement date: Jul 30, 2019 / Storage temperature: 4 °C / Cell temperature: 20 °C / Exposure time: 0.5 sec. / Number of frames: 1500 / Unit: 1/A /

	Min	Max
Q	0.0043	0.3522

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 1228 /

	Min	Max
Q	0.00425604	0.351957
P(R) point	1	1228
R	0	115

Result

Type of curve: sec /

	Experimental	Porod
MW	92 kDa	-
Volume	-	120 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.149	0.1485	0.0001028
Radius of gyration, Rg	3.53 nm	3.48 nm	0.01

	Min	Max
D	-	11.5
Guinier point	9	114