SASDG85: Wild-type full-length Liver Receptor Homolog-1/Peroxisome Prolife...

Basic information

Entry

Database: SASBDB / ID: SASDG85

Sample

Wild-type full-length Liver Receptor Homolog-1/Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha peptide complex bound to the CYP7A1 promoter oligonucleotide duplex.

• Liver Receptor Homolog-1 (protein), LRH-1Liver receptor homolog-1, Homo sapiens
• Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha (protein), PGC1 alpha, Homo sapiens
• CYP7A1 Promoter Forward (DNA), Homo sapiens
• CYP7A1 Promoter Reverse (DNA), Homo sapiens

Function / homology

Function:

Regulation of gene expression in early pancreatic precursor cells / pancreas morphogenesis / calcineurin-mediated signaling / acinar cell differentiation / tissue development / bile acid metabolic process / embryo development ending in birth or egg hatching / homeostatic process / positive regulation of viral genome replication / cellular response to leukemia inhibitory factor / hormone-mediated signaling pathway / cholesterol homeostasis / transcription coregulator binding / phospholipid binding / SUMOylation of intracellular receptors / Nuclear Receptor transcription pathway / RNA polymerase II transcription regulator complex / nuclear receptor activity / sequence-specific double-stranded DNA binding / regulation of cell population proliferation / DNA-binding transcription activator activity, RNA polymerase II-specific / Estrogen-dependent gene expression / sequence-specific DNA binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / chromatin binding / chromatin / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleoplasm / nucleus / cytoplasm

Domain/homology:

Nuclear hormone receptor family 5 / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Zinc finger, C4 type (two domains) / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors / Nuclear hormone receptor, ligand-binding domain / Nuclear hormone receptor-like domain superfamily / Ligand-binding domain of nuclear hormone receptor / Nuclear receptor (NR) ligand-binding (LBD) domain profile. / Ligand binding domain of hormone receptors / Zinc finger, NHR/GATA-type

Component:

Nuclear receptor subfamily 5 group A member 2

• Biological species: Homo sapiens (human)

Contact author

• Corey Seacrist (Vanderbilt University)

Models

Sample

• Sample: Name: Wild-type full-length Liver Receptor Homolog-1/Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha peptide complex bound to the CYP7A1 promoter oligonucleotide duplex.; Specimen concentration: 7.5 mg/ml / Entity id: 1884 / 1885 / 1886 / 1887
• Buffer: Name: 20 mM TRIS, 150 mM NaCl, 2% v/v glycerol, 0.5 mM CHAPS, 5 mM DTT; pH: 7.5

Entity #1884

Name: LRH-1Liver receptor homolog-1 / Type: protein / Description: Liver Receptor Homolog-1 / Formula weight: 64.144 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: O00482
Sequence:

MAHHHHHHVD DDDKMSENLY FQSMSSNSDT GDLQESLKHG LTPIGAGLPD RHGSPIPARG RLVMLPKVET EALGLARSHG EQGQMPENMQ VSQFKMVNYS YDEDLEELCP VCGDKVSGYH YGLLTCESCK GFFKRTVQNN KRYTCIENQN CQIDKTQRKR CPYCRFQKCL SVGMKLEAVR ADRMRGGRNK FGPMYKRDRA LKQQKKALIR ANGLKLEAMS QVIQAMPSDL TISSAIQNIH SASKGLPLNH AALPPTDYDR SPFVTSPISM TMPPHGSLQG YQTYGHFPSR AIKSEYPDPY TSSPESIMGY SYMDSYQTSS PASIPHLILE LLKCEPDEPQ VQAKIMAYLQ QEQANRSKHE KLSTFGLMCK MADQTLFSIV EWARSSIFFR ELKVDDQMKL LQNCWSELLI LDHIYRQVVH GKEGSIFLVT GQQVDYSIIA SQAGATLNNL MSHAQELVAK LRSLQFDQRE FVCLKFLVLF SLDVKNLENF QLVEGVQEQV NAALLDYTMC NYPQQTEKFG QLLLRLPEIR AISMQAEEYL YYKHLNGDVP YNNLLIEMLH AKRA

Entity #1885

Name: PGC1 alpha / Type: protein
Description: Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha
Formula weight: 1.522 / Num. of mol.: 1 / Source: Homo sapiens
Sequence:

EEPSLLKKLL LAPA

Entity #1886

Type: DNA / Description: CYP7A1 Promoter Forward / Formula weight: 3.75 / Num. of mol.: 1 / Source: Homo sapiens
Sequence:

GTTCAAGGCC AG

Entity #1887

Type: DNA / Description: CYP7A1 Promoter Reverse / Formula weight: 3.701 / Num. of mol.: 1 / Source: Homo sapiens
Sequence:

CTGGCCTTGA AC

Experimental information

• Beam: Instrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS); City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1127 Å / Dist. spec. to detc.: 2.105 mm
• Detector: Name: Pilatus3 X 2M / Pixsize x: 172 mm

Scan

Title: Wild-type full-length Liver Receptor Homolog-1/Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha peptide complex bound to the CYP7A1 promoter oligonucleotide duplex.
Measurement date: May 26, 2019 / Cell temperature: 20 °C / Exposure time: 3 sec. / Number of frames: 500 / Unit: 1/A /

	Min	Max
Q	0.0153	0.3704

Distance distribution function P(R)

Sofotware P(R): GNOM 4.6 / Number of points: 318 /

	Min	Max
Q	0.01561	0.3704
P(R) point	1	318
R	0	130

Result

Type of curve: sec /

	Experimental	Porod
MW	67 kDa	-
Volume	-	76 nm3

	P(R)	Guinier	Guinier error	P(R) error
Forward scattering, I0	23.72	23.89	0.22	-
Radius of gyration, Rg	3.907 nm	3.819 nm	0.046	0.02

	Min	Max
D	-	13
Guinier point	1	34