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- SASDG74: Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14... -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDG74
SampleActive dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)
  • Cytohesin-3 (protein), Cyth3, Grp1, Mus musculus
Function / homology
Function and homology information


Intra-Golgi traffic / Golgi vesicle transport / establishment of epithelial cell polarity / regulation of ARF protein signal transduction / phosphatidylinositol-3,4,5-trisphosphate binding / bicellular tight junction / ruffle / positive regulation of cell adhesion / guanyl-nucleotide exchange factor activity / adherens junction ...Intra-Golgi traffic / Golgi vesicle transport / establishment of epithelial cell polarity / regulation of ARF protein signal transduction / phosphatidylinositol-3,4,5-trisphosphate binding / bicellular tight junction / ruffle / positive regulation of cell adhesion / guanyl-nucleotide exchange factor activity / adherens junction / nucleoplasm / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Sec7 domain / Sec7, C-terminal domain superfamily / Sec7 domain superfamily / Sec7 domain / SEC7 domain profile. / Sec7 domain / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / PH-like domain superfamily
Similarity search - Domain/homology
Biological speciesMus musculus (house mouse)
Contact author
  • David Lambright (UMASS, UMASS Medical School, Worcester, MA, USA)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3642
Type: dummy / Software: (dammif (r7885)) / Radius of dummy atoms: 5.30 A / Symmetry: P1 / Comment: Most representative of 100 DAMMIF models / Chi-square value: 1.269 / P-value: 0.007914
Search similar-shape structures of this assembly by Omokage search (details)
Model #3686
Type: dummy / Software: (DAMFILT 5.0 (r7885)) / Radius of dummy atoms: 4.25 A / Symmetry: P1
Comment: Filtered average of 10 filtered averages of 10 DAMMIF models
Chi-square value: 1.269 / P-value: 0.007914
Search similar-shape structures of this assembly by Omokage search (details)
Model #3680
Type: dummy / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Comment: Most representative of 100 GASBOR models / Chi-square value: 1.66 / P-value: 0.000013
Search similar-shape structures of this assembly by Omokage search (details)
Model #3687
Type: dummy / Software: (DAMFILT 5.0 (r7885)) / Radius of dummy atoms: 3.00 A / Symmetry: P1
Comment: Filtered average of 10 filtered averages of 10 GASBOR models
Chi-square value: 1.66 / P-value: 0.000013
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)
Specimen concentration: 10 mg/ml
BufferName: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate
pH: 8
Entity #1856Name: Cyth3, Grp1 / Type: protein / Description: Cytohesin-3 / Formula weight: 45.153 / Num. of mol.: 2 / Source: Mus musculus / References: UniProt: O08967
Sequence: MGHHHHHHGS EDLSLEEREE LLDIRRRKKE LIDDIERLKY EIAEVMTEID NLTSVEESKT TQRNKQIAMG RKKFNMDPKK GIQFLIENDL LQSSPEDVAQ FLYKGEGLNK TVIGDYLGER DDFNIKVLQA FVELHEFADL NLVQALRQFL WSFRLPGEAQ KIDRMMEAFA ...Sequence:
MGHHHHHHGS EDLSLEEREE LLDIRRRKKE LIDDIERLKY EIAEVMTEID NLTSVEESKT TQRNKQIAMG RKKFNMDPKK GIQFLIENDL LQSSPEDVAQ FLYKGEGLNK TVIGDYLGER DDFNIKVLQA FVELHEFADL NLVQALRQFL WSFRLPGEAQ KIDRMMEAFA SRYCLCNPGV FQSTDTCYVL SFAIIMLNTS LHNHNVRDKP TAERFITMNR GINEGGDLPE ELLRNLYESI KNEPFKIPED DGNDLTHTFF NPDREGWLLK LGGRVKTWKR RWFILTDNCL YYFEYTTDKE PRGIIPLENL SIREVEDPRK PNCFELYNPS HKGQVIKACK TEADGRVVEG NHVVYRISAP SPEEKEEWMK SIKASISRDP FYDMLAT

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Experimental information

BeamInstrument name: Advanced Photon Source (APS), Argonne National Laboratory BioCAT 18ID
City: Lemont, IL / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.10332 Å / Dist. spec. to detc.: 2.5 mm
DetectorName: MAR 165 CCD
Scan
Title: Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)
Measurement date: Nov 15, 2013 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 126 / Unit: 1/A /
MinMax
Q0.0069 0.3162
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 76 /
MinMax
Q0.00688375 0.316224
P(R) point1 76
R0 257
Result
Type of curve: sec
Comments: The protein was equilibrated with a 1.2 molar excess of inositol 1,3,4,5-tetrakis phosphate (IP4) and concentrated to 10 mg/ml prior to injection.
ExperimentalPorod
MW86 kDa-
Volume-168 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I01.03 0.005 1.03 0.002
Radius of gyration, Rg5.43 nm0.14 5.3 nm0.04

MinMaxError
D-25.7 0.1
Guinier point1 20 -

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