SASDG69: Apolipoprotein E4 (K143A K146A) mutant (ApoE4 (K143A K146A))

Basic information

Entry

Database: SASBDB / ID: SASDG69

Sample

Apolipoprotein E4 (K143A K146A) mutant

• Apolipoprotein E4 (K143A K146A) mutant (protein), ApoE4 (K143A K146A), Homo sapiens

Function / homology

Function:

lipid transport involved in lipid storage / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / triglyceride-rich lipoprotein particle clearance / discoidal high-density lipoprotein particle / lipoprotein particle / negative regulation of triglyceride metabolic process / negative regulation of cholesterol biosynthetic process / regulation of amyloid-beta clearance / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / positive regulation of lipoprotein transport / chylomicron remnant clearance / chylomicron remnant / maintenance of location in cell / intermediate-density lipoprotein particle / very-low-density lipoprotein particle remodeling / acylglycerol homeostasis / NMDA glutamate receptor clustering / response to caloric restriction / very-low-density lipoprotein particle clearance / phosphatidylcholine-sterol O-acyltransferase activator activity / Chylomicron clearance / positive regulation of phospholipid efflux / lipid transporter activity / Chylomicron remodeling / positive regulation of cholesterol metabolic process / regulation of amyloid fibril formation / regulation of behavioral fear response / cellular response to lipoprotein particle stimulus / positive regulation of low-density lipoprotein particle receptor catabolic process / regulation of protein metabolic process / Chylomicron assembly / high-density lipoprotein particle clearance / high-density lipoprotein particle remodeling / chylomicron / phospholipid efflux / AMPA glutamate receptor clustering / very-low-density lipoprotein particle receptor binding / lipoprotein catabolic process / multivesicular body, internal vesicle / melanosome organization / reverse cholesterol transport / high-density lipoprotein particle assembly / positive regulation of amyloid-beta clearance / positive regulation by host of viral process / : / low-density lipoprotein particle / protein import / lipoprotein biosynthetic process / cholesterol transfer activity / high-density lipoprotein particle / very-low-density lipoprotein particle / low-density lipoprotein particle remodeling / heparan sulfate proteoglycan binding / negative regulation of amyloid fibril formation / regulation of Cdc42 protein signal transduction / amyloid precursor protein metabolic process / triglyceride homeostasis / regulation of amyloid precursor protein catabolic process / cholesterol catabolic process / positive regulation of membrane protein ectodomain proteolysis / synaptic transmission, cholinergic / HDL remodeling / negative regulation of endothelial cell migration / cholesterol efflux / regulation of axon extension / artery morphogenesis / Scavenging by Class A Receptors / negative regulation of protein metabolic process / regulation of cholesterol metabolic process / positive regulation of dendritic spine development / triglyceride metabolic process / low-density lipoprotein particle receptor binding / positive regulation of amyloid fibril formation / regulation of innate immune response / virion assembly / negative regulation of amyloid-beta formation / locomotory exploration behavior / negative regulation of endothelial cell proliferation / antioxidant activity / positive regulation of endocytosis / lipoprotein particle binding / response to dietary excess / regulation of neuronal synaptic plasticity / negative regulation of blood vessel endothelial cell migration / negative regulation of long-term synaptic potentiation / negative regulation of platelet activation / positive regulation of cholesterol efflux / positive regulation of dendritic spine maintenance / negative regulation of blood coagulation / negative regulation of MAP kinase activity / long-term memory / negative regulation of protein secretion / intracellular transport / fatty acid homeostasis / regulation of protein-containing complex assembly / long-chain fatty acid transport / synaptic cleft

Domain/homology:

Apolipoprotein A/E / : / Apolipoprotein A1/A4/E domain

Component:

Apolipoprotein E

• Biological species: Homo sapiens (human)

Contact author

• Lucas Kraft (University of Sussex, Brighton, UK)

Models

Sample

• Sample: Name: Apolipoprotein E4 (K143A K146A) mutant / Specimen concentration: 4 mg/ml
• Buffer: Name: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

Entity #1634

Name: ApoE4 (K143A K146A) / Type: protein / Description: Apolipoprotein E4 (K143A K146A) mutant / Formula weight: 34.598 / Num. of mol.: 4 / Source: Homo sapiens / References: UniProt: P02649
Sequence:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVRGRLV QYRGEVQAML GQSTEELRVR LASHLRALRA RLLRDADDLQ KRLAVYQAGA REGAERGLSA IRERLGPLVE QGRVRAATVG SLAGQPLQER AQAWGERLRA RMEEMGSRTR DRLDEVKEQV AEVRAKLEEQ AQQIRLQAEA FQARLKSWFE PLVEDMQRQW AGLVEKVQAA VGTSAAPVPS DNH

Experimental information

• Beam: Instrument name: Diamond Light Source B21 / City: Didcot / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.036 mm
• Detector: Name: Pilatus 2M

Scan

Title: Apolipoprotein E4 (K143A K146A) mutant / Measurement date: Jul 21, 2018 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 28 / Unit: 1/A /

	Min	Max
Q	0.0035	0.37

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 976 /

	Min	Max
Q	0.00884386	0.225938
P(R) point	1	976
R	0	207

Result

Experimental MW: 159 kDa / Type of curve: single_conc / Comments: ApoE4 (K143A K146A)

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.3408	0.34	0.0031
Radius of gyration, Rg	6.24 nm	6.158 nm	0.089

	Min	Max
D	-	20.7
Guinier point	25	73