+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDG57 |
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Sample | 1:2 heterotrimer of pUL7 and pUL51 from herpes simplex virus 1
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Function / homology | Herpesvirus UL51 / Herpesvirus UL51 protein / Herpesvirus UL7-like / Herpesvirus UL7 like / viral tegument / host cell Golgi apparatus / cytoplasm / UL7 / UL51 Function and homology information |
Biological species | Human alphaherpesvirus 1 strain KOS Human alphaherpesvirus 1 (Herpes simplex virus type 1) |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Data source
SASBDB page | SASDG57 |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Models
Model #3908 | Type: dummy / Radius of dummy atoms: 1.90 A / Comment: Monomer component fit, 80% volume fraction / Chi-square value: 1.08 / P-value: 0.131158 |
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Model #3909 | Type: dummy / Radius of dummy atoms: 1.90 A / Symmetry: P2 / Comment: Dimer component fit, 20% volume fraction / Chi-square value: 1.00 / P-value: 0.363345 |
-Sample
Sample | Name: 1:2 heterotrimer of pUL7 and pUL51 from herpes simplex virus 1 Specimen concentration: 8 mg/ml / Entity id: 1939 / 1940 |
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Buffer | Name: 20 mM HEPES, 200 mM NaCl, 3% (v/v) glycerol, 1 mM DTT / pH: 7.5 |
Entity #1939 | Name: pUL7 / Type: protein / Description: Tegument protein UL7 / Formula weight: 33.907 / Num. of mol.: 1 / Source: Human alphaherpesvirus 1 strain KOS / References: UniProt: A0A110B4Q7 Sequence: MAAATADDEG SAATILKQAI AGDRSLVEAA EAISQQTLLR LACEVRQVGD RQPRFTATSI ARVDVAPGCR LRFVLDGSPE DAYVTSEDYF KRCCGQSSYR GFAVAVLTAN EDHVHSLAVP PLVLLHRFSL FNPRDLLDFE LACLLMYLEN CPRSHATPST FAKVLAWLGV ...Sequence: MAAATADDEG SAATILKQAI AGDRSLVEAA EAISQQTLLR LACEVRQVGD RQPRFTATSI ARVDVAPGCR LRFVLDGSPE DAYVTSEDYF KRCCGQSSYR GFAVAVLTAN EDHVHSLAVP PLVLLHRFSL FNPRDLLDFE LACLLMYLEN CPRSHATPST FAKVLAWLGV AGRRTSPFER VRCLFLRSCH WVLNTLMFMV HVKPFDDEFV LPHWYMARYL LANNPPPVLS ALFCATPTSS SFRLPGPPPR SDCVAYNPAG IMGSCWASEE VRAPLVYWWL SETPKRQTSS LFYQFCGSLE VLFQ |
Entity #1940 | Name: pUL51 / Type: protein / Description: Tegument protein UL51 / Formula weight: 25.448 / Num. of mol.: 2 / Source: Human alphaherpesvirus 1 / References: UniProt: D3YPL0 Sequence: MASLLGAISG WGARPEEQYE MIRAAVPPSE AEPRLQEALV VVNALLPAPI TLDDALGSLD DTRRLVKARA LARTYHACMV NLERLARHHP GLEAPTIDGA VAAHQDKMRR LADTCMATIL QMYMSVGAAD KSADVLVSQA IRSMAESDVV MEDVAIAERA LGLSAFGVAG ...Sequence: MASLLGAISG WGARPEEQYE MIRAAVPPSE AEPRLQEALV VVNALLPAPI TLDDALGSLD DTRRLVKARA LARTYHACMV NLERLARHHP GLEAPTIDGA VAAHQDKMRR LADTCMATIL QMYMSVGAAD KSADVLVSQA IRSMAESDVV MEDVAIAERA LGLSAFGVAG GTRSGGLGVT EAPSLGHPHT PPPEVTLAPA ARNGDALPDP KPESCPRVSV PRPTASPTAP RPGPSRAAPC VLGQ |
-Experimental information
Beam | Instrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3 mm | ||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 6M | ||||||||||||||||||||||||||||||
Scan | Title: 1:2 heterotrimer of pUL7 and pUL51 from herpes simplex virus 1 Measurement date: May 16, 2019 / Cell temperature: 20.2 °C / Exposure time: 0.995 sec. / Number of frames: 35 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 1162 /
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Result | Type of curve: sec Comments: The quoted experimental molecular mass, that corresponds to a 1:2 pUL7:pUL51 heterotrimer, was determined from SEC-MALLS-RI measurements performed in parallel to the SEC-SAXS. The SEC-SAXS ...Comments: The quoted experimental molecular mass, that corresponds to a 1:2 pUL7:pUL51 heterotrimer, was determined from SEC-MALLS-RI measurements performed in parallel to the SEC-SAXS. The SEC-SAXS data, Rg correlations through the heterotrimer elution peak as well as the the SEC-MALLS-RI data are made available in the full-entry zip archive. The GASBORMX model displayed in this entry represents an 80 % volume fraction of the heterotrimer (corresponding fit, top) combined with a 20% volume fraction of a 2:4 pUL7:pUL51 heterohexamer (corresponding fit, bottom), due to incomplete SEC-separation between the two forms of the complex (also refer to SASBDB entry SASDG47).
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