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- SASDG57: 1:2 heterotrimer of pUL7 and pUL51 from herpes simplex virus 1 -

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Basic information

Entry
Database: SASBDB / ID: SASDG57
Sample1:2 heterotrimer of pUL7 and pUL51 from herpes simplex virus 1
  • Tegument protein UL7 (protein), pUL7, Human alphaherpesvirus 1 strain KOS
  • Tegument protein UL51 (protein), pUL51, Human alphaherpesvirus 1
Function / homologyHerpesvirus UL51 / Herpesvirus UL51 protein / Herpesvirus UL7-like / Herpesvirus UL7 like / viral tegument / host cell Golgi apparatus / cytoplasm / UL7 / UL51
Function and homology information
Biological speciesHuman alphaherpesvirus 1 strain KOS
Human alphaherpesvirus 1 (Herpes simplex virus type 1)
Contact author
  • Benjamin Butt (University of Cambridge)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3908
Type: dummy / Radius of dummy atoms: 1.90 A / Comment: Monomer component fit, 80% volume fraction / Chi-square value: 1.08 / P-value: 0.131158
Model #3909
Type: dummy / Radius of dummy atoms: 1.90 A / Symmetry: P2 / Comment: Dimer component fit, 20% volume fraction / Chi-square value: 1.00 / P-value: 0.363345

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Sample

SampleName: 1:2 heterotrimer of pUL7 and pUL51 from herpes simplex virus 1
Specimen concentration: 8 mg/ml / Entity id: 1939 / 1940
BufferName: 20 mM HEPES, 200 mM NaCl, 3% (v/v) glycerol, 1 mM DTT / pH: 7.5
Entity #1939Name: pUL7 / Type: protein / Description: Tegument protein UL7 / Formula weight: 33.907 / Num. of mol.: 1 / Source: Human alphaherpesvirus 1 strain KOS / References: UniProt: A0A110B4Q7
Sequence: MAAATADDEG SAATILKQAI AGDRSLVEAA EAISQQTLLR LACEVRQVGD RQPRFTATSI ARVDVAPGCR LRFVLDGSPE DAYVTSEDYF KRCCGQSSYR GFAVAVLTAN EDHVHSLAVP PLVLLHRFSL FNPRDLLDFE LACLLMYLEN CPRSHATPST FAKVLAWLGV ...Sequence:
MAAATADDEG SAATILKQAI AGDRSLVEAA EAISQQTLLR LACEVRQVGD RQPRFTATSI ARVDVAPGCR LRFVLDGSPE DAYVTSEDYF KRCCGQSSYR GFAVAVLTAN EDHVHSLAVP PLVLLHRFSL FNPRDLLDFE LACLLMYLEN CPRSHATPST FAKVLAWLGV AGRRTSPFER VRCLFLRSCH WVLNTLMFMV HVKPFDDEFV LPHWYMARYL LANNPPPVLS ALFCATPTSS SFRLPGPPPR SDCVAYNPAG IMGSCWASEE VRAPLVYWWL SETPKRQTSS LFYQFCGSLE VLFQ
Entity #1940Name: pUL51 / Type: protein / Description: Tegument protein UL51 / Formula weight: 25.448 / Num. of mol.: 2 / Source: Human alphaherpesvirus 1 / References: UniProt: D3YPL0
Sequence: MASLLGAISG WGARPEEQYE MIRAAVPPSE AEPRLQEALV VVNALLPAPI TLDDALGSLD DTRRLVKARA LARTYHACMV NLERLARHHP GLEAPTIDGA VAAHQDKMRR LADTCMATIL QMYMSVGAAD KSADVLVSQA IRSMAESDVV MEDVAIAERA LGLSAFGVAG ...Sequence:
MASLLGAISG WGARPEEQYE MIRAAVPPSE AEPRLQEALV VVNALLPAPI TLDDALGSLD DTRRLVKARA LARTYHACMV NLERLARHHP GLEAPTIDGA VAAHQDKMRR LADTCMATIL QMYMSVGAAD KSADVLVSQA IRSMAESDVV MEDVAIAERA LGLSAFGVAG GTRSGGLGVT EAPSLGHPHT PPPEVTLAPA ARNGDALPDP KPESCPRVSV PRPTASPTAP RPGPSRAAPC VLGQ

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3 mm
DetectorName: Pilatus 6M
Scan
Title: 1:2 heterotrimer of pUL7 and pUL51 from herpes simplex virus 1
Measurement date: May 16, 2019 / Cell temperature: 20.2 °C / Exposure time: 0.995 sec. / Number of frames: 35 / Unit: 1/nm /
MinMax
Q0.0225 7.3148
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1162 /
MinMax
Q0.127778 3.34573
P(R) point1 1162
R0 18.22
Result
Type of curve: sec
Comments: The quoted experimental molecular mass, that corresponds to a 1:2 pUL7:pUL51 heterotrimer, was determined from SEC-MALLS-RI measurements performed in parallel to the SEC-SAXS. The SEC-SAXS ...Comments: The quoted experimental molecular mass, that corresponds to a 1:2 pUL7:pUL51 heterotrimer, was determined from SEC-MALLS-RI measurements performed in parallel to the SEC-SAXS. The SEC-SAXS data, Rg correlations through the heterotrimer elution peak as well as the the SEC-MALLS-RI data are made available in the full-entry zip archive. The GASBORMX model displayed in this entry represents an 80 % volume fraction of the heterotrimer (corresponding fit, top) combined with a 20% volume fraction of a 2:4 pUL7:pUL51 heterohexamer (corresponding fit, bottom), due to incomplete SEC-separation between the two forms of the complex (also refer to SASBDB entry SASDG47).
ExperimentalPorod
MW84 kDa100 kDa
Volume-160 nm3

P(R)GuinierGuinier error
Forward scattering, I04643 4538.44 9.56
Radius of gyration, Rg4.296 nm3.95 nm0.07

MinMax
D-18.22
Guinier point39 108

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