SASDG55: Deglycosylated latency associated peptide, LAP (TGFB-1)

基本情報

登録情報

データベース: SASBDB / ID: SASDG55

試料

Deglycosylated latency associated peptide, LAP (TGFB-1)

• Latency associated peptide (protein), LAP, Homo sapiens

機能・相同性

分子機能:

cellular response to acetaldehyde / adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains / positive regulation of microglia differentiation / regulation of interleukin-23 production / branch elongation involved in mammary gland duct branching / positive regulation of primary miRNA processing / Influenza Virus Induced Apoptosis / negative regulation of skeletal muscle tissue development / regulation of branching involved in mammary gland duct morphogenesis / macrophage derived foam cell differentiation / frontal suture morphogenesis / regulation of enamel mineralization / regulation of cartilage development / TGFBR2 MSI Frameshift Mutants in Cancer / regulation of striated muscle tissue development / regulatory T cell differentiation / tolerance induction to self antigen / regulation of blood vessel remodeling / regulation of protein import into nucleus / embryonic liver development / extracellular matrix assembly / negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target / columnar/cuboidal epithelial cell maturation / negative regulation of hyaluronan biosynthetic process / type III transforming growth factor beta receptor binding / positive regulation of cardiac muscle cell differentiation / myofibroblast differentiation / odontoblast differentiation / positive regulation of odontogenesis / connective tissue replacement involved in inflammatory response wound healing / Langerhans cell differentiation / negative regulation of macrophage cytokine production / positive regulation of smooth muscle cell differentiation / TGFBR2 Kinase Domain Mutants in Cancer / positive regulation of exit from mitosis / positive regulation of isotype switching to IgA isotypes / positive regulation of mesenchymal stem cell proliferation / SMAD2/3 Phosphorylation Motif Mutants in Cancer / TGFBR1 KD Mutants in Cancer / membrane protein intracellular domain proteolysis / positive regulation of receptor signaling pathway via STAT / heart valve morphogenesis / retina vasculature development in camera-type eye / TGFBR3 regulates TGF-beta signaling / mammary gland branching involved in thelarche / bronchiole development / hyaluronan catabolic process / positive regulation of vasculature development / response to laminar fluid shear stress / lens fiber cell differentiation / positive regulation of extracellular matrix assembly / negative regulation of extracellular matrix disassembly / ATP biosynthetic process / positive regulation of branching involved in ureteric bud morphogenesis / receptor catabolic process / type II transforming growth factor beta receptor binding / TGFBR1 LBD Mutants in Cancer / oligodendrocyte development / type I transforming growth factor beta receptor binding / response to salt / germ cell migration / negative regulation of biomineral tissue development / positive regulation of mononuclear cell migration / endoderm development / phospholipid homeostasis / negative regulation of myoblast differentiation / positive regulation of chemotaxis / negative regulation of cell-cell adhesion mediated by cadherin / cell-cell junction organization / response to vitamin D / response to cholesterol / positive regulation of vascular permeability / negative regulation of interleukin-17 production / surfactant homeostasis / deubiquitinase activator activity / phosphate-containing compound metabolic process / negative regulation of release of sequestered calcium ion into cytosol / positive regulation of chemokine (C-X-C motif) ligand 2 production / digestive tract development / positive regulation of fibroblast migration / aortic valve morphogenesis / sprouting angiogenesis / negative regulation of ossification / face morphogenesis / RUNX3 regulates CDKN1A transcription / neural tube development / positive regulation of regulatory T cell differentiation / ureteric bud development / Molecules associated with elastic fibres / positive regulation of epidermal growth factor receptor signaling pathway / negative regulation of phagocytosis / positive regulation of peptidyl-tyrosine phosphorylation / negative regulation of neuroblast proliferation / muscle cell cellular homeostasis / cellular response to insulin-like growth factor stimulus / Syndecan interactions / ventricular cardiac muscle tissue morphogenesis / lung alveolus development / negative regulation of fat cell differentiation / positive regulation of interleukin-17 production

ドメイン・相同性:

Transforming growth factor beta-1 proprotein / Transforming growth factor-beta / TGF-beta, propeptide / TGF-beta propeptide / Transforming growth factor beta, conserved site / TGF-beta family signature. / Transforming growth factor-beta-related / Transforming growth factor-beta (TGF-beta) family / Transforming growth factor-beta, C-terminal / Transforming growth factor beta like domain / TGF-beta family profile. / Cystine-knot cytokine

構成要素:

Transforming growth factor beta-1 proprotein

• 生物種: Homo sapiens (ヒト)

登録者

• Tim Stachowski (Hauptman-Woodward Medical Research Institute, New York, USA)

モデル

試料

• 試料: 名称: Deglycosylated latency associated peptide, LAP (TGFB-1); 試料濃度: 1.4 mg/ml
• バッファ: 名称: phosphate buffered saline 2% glycerol / pH: 7.4

要素 #1879

名称: LAP / タイプ: protein / 記述: Latency associated peptide / 分子量: 29.228 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: P01137
配列:

LSTCKTIDME LVKRKRIEAI RGQILSKLRL ASPPSQGEVP PGPLPEAVLA LYNSTRDRVA GESAEPEPEP EADYYAKEVT RVLMVETHNE IYDKFKQSTH SIYMFFNTSE LREAVPEPVL LSRAELRLLR LKLKVEQHVE LYQKYSNNSW RYLSNRLLAP SDSPEWLSFD VTGVVRQWLS RGGEIEGFRL SAHCSCDSRD NTLQVDINGF TTGRRGDLAT IHGMNRPFLL LMATPLERAQ HLQSSRHRAH HHHHH

実験情報

• ビーム: 設備名称: Advanced Light Source (ALS) 12.3.1 (SIBYLS) / 地域: Berkeley, CA / 国: USA / 線源: X-ray synchrotron / 波長: 0.127 Å / スペクトロメータ・検出器間距離: 2 mm
• 検出器: 名称: Pilatus3 X 2M / Pixsize x: 172 mm

スキャン

タイトル: Deglycosylated latency associated peptide, LAP (TGFB-1)
測定日: 2019年4月20日 / 保管温度: 4 °C / セル温度: 10 °C / 照射時間: 0.1 sec. / フレーム数: 100 / 単位: 1/A /

	Min	Max
Q	0.0109	0.2611

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 446 /

	Min	Max
Q	0.0131	0.261142
P(R) point	1	446
R	0	130

結果

D max: 13 / カーブのタイプ: single_conc

	実験値	Standard	Standard error	Porod
分子量	59.1 kDa	47 kDa	5	-
体積	-	-	-	129 nm3

	P(R)	Guinier	Guinier error
前方散乱 I0	1.987	1.99	0.01
慣性半径, Rg	3.55 nm	3.51 nm	0.11