SASDG49: Apolipoprotein E3 (ApoE3)

Basic information

Entry

Database: SASBDB / ID: SASDG49

Sample

Apolipoprotein E3

• Apolipoprotein E3 (protein), ApoE3, Homo sapiens

Function / homology

Function:

lipid transport involved in lipid storage / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / triglyceride-rich lipoprotein particle clearance / discoidal high-density lipoprotein particle / lipoprotein particle / negative regulation of triglyceride metabolic process / maintenance of location in cell / regulation of amyloid-beta clearance / negative regulation of cholesterol biosynthetic process / positive regulation of lipoprotein transport / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / chylomicron remnant clearance / chylomicron remnant / intermediate-density lipoprotein particle / acylglycerol homeostasis / NMDA glutamate receptor clustering / very-low-density lipoprotein particle remodeling / Chylomicron clearance / phosphatidylcholine-sterol O-acyltransferase activator activity / positive regulation of phospholipid efflux / Chylomicron remodeling / positive regulation of low-density lipoprotein particle receptor catabolic process / response to caloric restriction / cellular response to lipoprotein particle stimulus / lipid transporter activity / very-low-density lipoprotein particle clearance / Chylomicron assembly / high-density lipoprotein particle clearance / phospholipid efflux / chylomicron / regulation of protein metabolic process / very-low-density lipoprotein particle receptor binding / regulation of amyloid fibril formation / lipoprotein catabolic process / AMPA glutamate receptor clustering / high-density lipoprotein particle remodeling / melanosome organization / positive regulation of cholesterol metabolic process / multivesicular body, internal vesicle / regulation of behavioral fear response / reverse cholesterol transport / positive regulation of amyloid-beta clearance / high-density lipoprotein particle assembly / low-density lipoprotein particle / host-mediated activation of viral process / lipoprotein biosynthetic process / cholesterol transfer activity / high-density lipoprotein particle / protein import / very-low-density lipoprotein particle / cholesterol catabolic process / heparan sulfate proteoglycan binding / low-density lipoprotein particle remodeling / amyloid precursor protein metabolic process / negative regulation of amyloid fibril formation / regulation of Cdc42 protein signal transduction / positive regulation of membrane protein ectodomain proteolysis / synaptic transmission, cholinergic / regulation of amyloid precursor protein catabolic process / HDL remodeling / negative regulation of endothelial cell migration / cholesterol efflux / regulation of cholesterol metabolic process / artery morphogenesis / regulation of axon extension / negative regulation of protein metabolic process / Scavenging by Class A Receptors / triglyceride homeostasis / triglyceride metabolic process / low-density lipoprotein particle receptor binding / positive regulation of amyloid fibril formation / regulation of innate immune response / virion assembly / positive regulation of dendritic spine development / negative regulation of endothelial cell proliferation / response to dietary excess / antioxidant activity / negative regulation of MAP kinase activity / negative regulation of amyloid-beta formation / lipoprotein particle binding / locomotory exploration behavior / negative regulation of long-term synaptic potentiation / positive regulation of endocytosis / negative regulation of blood vessel endothelial cell migration / negative regulation of platelet activation / negative regulation of blood coagulation / positive regulation of dendritic spine maintenance / regulation of neuronal synaptic plasticity / positive regulation of cholesterol efflux / negative regulation of protein secretion / fatty acid homeostasis / long-term memory / regulation of protein-containing complex assembly / synaptic cleft / long-chain fatty acid transport / intracellular transport / positive regulation of lipid biosynthetic process

Domain/homology:

Apolipoprotein A/E / : / Apolipoprotein A1/A4/E domain

Component:

Apolipoprotein E

• Biological species: Homo sapiens (human)

Contact author

• Lucas Kraft (University of Sussex, Brighton, UK)

Models

Sample

• Sample: Name: Apolipoprotein E3 / Specimen concentration: 4 mg/ml
• Buffer: Name: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

Entity #1108

Name: ApoE3 / Type: protein / Description: Apolipoprotein E3 / Formula weight: 34.659 / Num. of mol.: 4 / Source: Homo sapiens / References: UniProt: P02649
Sequence:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVCGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KRLAVYQAGA REGAERGLSA IRERLGPLVE QGRVRAATVG SLAGQPLQER AQAWGERLRA RMEEMGSRTR DRLDEVKEQV AEVRAKLEEQ AQQIRLQAEA FQARLKSWFE PLVEDMQRQW AGLVEKVQAA VGTSAAPVPS DNH

Experimental information

• Beam: Instrument name: Diamond Light Source B21 / City: Didcot / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.036 mm
• Detector: Name: Pilatus 2M

Scan

Title: Apolipoprotein E3 / Measurement date: May 3, 2018 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 28 / Unit: 1/A /

	Min	Max
Q	0.003	0.37

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 967 /

	Min	Max
Q	0.0103445	0.225337
P(R) point	1	967
R	0	206

Result

Experimental MW: 177 kDa / Type of curve: single_conc / Comments: ApoE3

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.4452	0.44	0.0036
Radius of gyration, Rg	6.29 nm	6.14 nm	0.069

	Min	Max
D	-	20.6
Guinier point	34	82