SASDG48: Apolipoprotein E4 (methylated) bound to 4 µg/mL heparin

基本情報

登録情報

データベース: SASBDB / ID: SASDG48

試料

Apolipoprotein E4 (methylated) bound to 4 µg/mL heparin

• Apolipoprotein E4 (protein), ApoE4, Homo sapiens
• Heparinヘパリン (other)

機能・相同性

分子機能:

chylomicron remnant / lipid transport involved in lipid storage / triglyceride-rich lipoprotein particle clearance / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / positive regulation of heparan sulfate proteoglycan binding / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / discoidal high-density lipoprotein particle / chylomicron remnant clearance / リポタンパク質 / maintenance of location in cell / very-low-density lipoprotein particle clearance / intermediate-density lipoprotein particle / very-low-density lipoprotein particle remodeling / regulation of amyloid-beta clearance / response to caloric restriction / Chylomicron clearance / negative regulation of triglyceride metabolic process / NMDA glutamate receptor clustering / カイロミクロン / Chylomicron remodeling / phosphatidylcholine-sterol O-acyltransferase activator activity / positive regulation of phospholipid efflux / Chylomicron assembly / lipid transporter activity / positive regulation of low-density lipoprotein particle receptor catabolic process / positive regulation of cholesterol metabolic process / regulation of behavioral fear response / high-density lipoprotein particle remodeling / high-density lipoprotein particle clearance / multivesicular body, internal vesicle / lipoprotein catabolic process / very-low-density lipoprotein particle receptor binding / phospholipid efflux / regulation of amyloid fibril formation / regulation of protein metabolic process / AMPA glutamate receptor clustering / low-density lipoprotein particle / positive regulation by host of viral process / cholesterol transfer activity / reverse cholesterol transport / positive regulation of amyloid-beta clearance / high-density lipoprotein particle assembly / very-low-density lipoprotein particle / positive regulation of CoA-transferase activity / melanosome organization / lipoprotein biosynthetic process / protein import / negative regulation of blood coagulation / low-density lipoprotein particle remodeling / high-density lipoprotein particle / negative regulation of amyloid fibril formation / negative regulation of cholesterol biosynthetic process / amyloid precursor protein metabolic process / heparan sulfate proteoglycan binding / regulation of Cdc42 protein signal transduction / triglyceride homeostasis / regulation of amyloid precursor protein catabolic process / cholesterol catabolic process / synaptic transmission, cholinergic / positive regulation of membrane protein ectodomain proteolysis / HDL remodeling / negative regulation of endothelial cell migration / cholesterol efflux / Scavenging by Class A Receptors / negative regulation of protein metabolic process / artery morphogenesis / triglyceride metabolic process / regulation of axon extension / regulation of cholesterol metabolic process / positive regulation of amyloid fibril formation / low-density lipoprotein particle receptor binding / positive regulation of dendritic spine development / virion assembly / regulation of innate immune response / regulation of neuronal synaptic plasticity / locomotory exploration behavior / lipoprotein particle binding / positive regulation of endocytosis / negative regulation of amyloid-beta formation / negative regulation of endothelial cell proliferation / antioxidant activity / cGMP-mediated signaling / response to dietary excess / negative regulation of blood vessel endothelial cell migration / negative regulation of long-term synaptic potentiation / negative regulation of platelet activation / positive regulation of cholesterol efflux / positive regulation of dendritic spine maintenance / intracellular transport / regulation of protein-containing complex assembly / negative regulation of protein secretion / long-term memory / fatty acid homeostasis / long-chain fatty acid transport / positive regulation of lipid biosynthetic process / synaptic cleft / regulation of proteasomal protein catabolic process

ドメイン・相同性:

Apolipoprotein A/E / Apolipoprotein A1/A4/E domain

構成要素:

アポリポプロテインE

• 生物種: Homo sapiens (ヒト)

登録者

• Lucas Kraft (University of Sussex, Brighton, UK)

モデル

試料

• 試料: 名称: Apolipoprotein E4 (methylated) bound to 4 µg/mL heparin; 試料濃度: 4 mg/ml / Entity id: 1109 / 1633
• バッファ: 名称: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

要素 #1109

名称: ApoE4 / タイプ: protein / 記述: Apolipoprotein E4 / 分子量: 34.712 / 分子数: 4 / 由来: Homo sapiens / 参照: UniProt: P02649
配列:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVRGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KRLAVYQAGA REGAERGLSA IRERLGPLVE QGRVRAATVG SLAGQPLQER AQAWGERLRA RMEEMGSRTR DRLDEVKEQV AEVRAKLEEQ AQQIRLQAEA FQARLKSWFE PLVEDMQRQW AGLVEKVQAA VGTSAAPVPS DNH

要素 #1633

タイプ: other / 記述: Heparinヘパリン / 分子量: 15 / 分子数: 1
配列:

(C12H19NO20S3)n

実験情報

• ビーム: 設備名称: Diamond Light Source B21 / 地域: Didcot / 国: UK / 形状: 1 x 5 mm / 線源: X-ray synchrotronシンクロトロン / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 4.036 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Apolipoprotein E4 (methylated) bound to 4 µg/mL heparin
測定日: 2018年5月3日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 28 / 単位: 1/A /

	Min	Max
Q	0.003	0.37

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 978 /

	Min	Max
Q	0.00611583	0.223556
P(R) point	1	978
R	0	202

結果

Experimental MW: 173 kDa / カーブのタイプ: single_conc
コメント: ApoE4 (methylated) + 4 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.

	P(R)	Guinier	Guinier error
前方散乱 I0	0.4354	0.43	0.0025
慣性半径, Rg	6.27 nm	6.113 nm	0.054

	Min	Max
D	-	20.2
Guinier point	15	82