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- SASDG47: 2:4 heterohexamer of pUL7 and pUL51 from herpes simplex virus 1 -

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Basic information

Entry
Database: SASBDB / ID: SASDG47
Sample2:4 heterohexamer of pUL7 and pUL51 from herpes simplex virus 1
  • Tegument protein UL7 (protein), pUL7, Human alphaherpesvirus 1 strain KOS
  • Tegument protein UL51 (protein), pUL51, Human alphaherpesvirus 1 strain KOS
Function / homologyHerpesvirus UL51 / Herpesvirus UL51 protein / Herpesvirus UL7-like / Herpesvirus UL7 like / viral tegument / host cell Golgi apparatus / cytoplasm / UL7 / UL51
Function and homology information
Biological speciesHuman alphaherpesvirus 1 strain KOS
Contact author
  • Benjamin Butt (University of Cambridge)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #3903
Type: dummy / Software: (SUPCOMB 23 (r10552)) / Radius of dummy atoms: 3.75 A / Symmetry: P2
Comment: Refined DAMMIN model of the HSV-1 pUL7:pUL51 2:4 heterohexamer
Chi-square value: 0.910 / P-value: 0.805657
Model #3904
Type: dummy / Software: (2.3i) / Radius of dummy atoms: 1.90 A / Symmetry: P2
Comment: Representative GASBOR model of the HSV-1 pUL7:pUL51 2:4 heterohexamer
Chi-square value: 1.00 / P-value: 0.363345

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Sample

SampleName: 2:4 heterohexamer of pUL7 and pUL51 from herpes simplex virus 1
Specimen concentration: 8 mg/ml / Entity id: 1941 / 1942
BufferName: 20 mM HEPES, 200 mM NaCl, 3% (v/v) glycerol, 1 mM DTT / pH: 7.5
Entity #1941Name: pUL7 / Type: protein / Description: Tegument protein UL7 / Formula weight: 33.907 / Num. of mol.: 2 / Source: Human alphaherpesvirus 1 strain KOS / References: UniProt: A0A110B4Q7
Sequence: MAAATADDEG SAATILKQAI AGDRSLVEAA EAISQQTLLR LACEVRQVGD RQPRFTATSI ARVDVAPGCR LRFVLDGSPE DAYVTSEDYF KRCCGQSSYR GFAVAVLTAN EDHVHSLAVP PLVLLHRFSL FNPRDLLDFE LACLLMYLEN CPRSHATPST FAKVLAWLGV ...Sequence:
MAAATADDEG SAATILKQAI AGDRSLVEAA EAISQQTLLR LACEVRQVGD RQPRFTATSI ARVDVAPGCR LRFVLDGSPE DAYVTSEDYF KRCCGQSSYR GFAVAVLTAN EDHVHSLAVP PLVLLHRFSL FNPRDLLDFE LACLLMYLEN CPRSHATPST FAKVLAWLGV AGRRTSPFER VRCLFLRSCH WVLNTLMFMV HVKPFDDEFV LPHWYMARYL LANNPPPVLS ALFCATPTSS SFRLPGPPPR SDCVAYNPAG IMGSCWASEE VRAPLVYWWL SETPKRQTSS LFYQFCGSLE VLFQ
Entity #1942Name: pUL51 / Type: protein / Description: Tegument protein UL51 / Formula weight: 25.448 / Num. of mol.: 4 / Source: Human alphaherpesvirus 1 strain KOS / References: UniProt: D3YPL0
Sequence: MASLLGAISG WGARPEEQYE MIRAAVPPSE AEPRLQEALV VVNALLPAPI TLDDALGSLD DTRRLVKARA LARTYHACMV NLERLARHHP GLEAPTIDGA VAAHQDKMRR LADTCMATIL QMYMSVGAAD KSADVLVSQA IRSMAESDVV MEDVAIAERA LGLSAFGVAG ...Sequence:
MASLLGAISG WGARPEEQYE MIRAAVPPSE AEPRLQEALV VVNALLPAPI TLDDALGSLD DTRRLVKARA LARTYHACMV NLERLARHHP GLEAPTIDGA VAAHQDKMRR LADTCMATIL QMYMSVGAAD KSADVLVSQA IRSMAESDVV MEDVAIAERA LGLSAFGVAG GTRSGGLGVT EAPSLGHPHT PPPEVTLAPA ARNGDALPDP KPESCPRVSV PRPTASPTAP RPGPSRAAPC VLGQ

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3 mm
DetectorName: Pilatus 6M
Scan
Title: 2:4 heterohexamer of pUL7 and pUL51 from herpes simplex virus 1
Measurement date: May 16, 2019 / Cell temperature: 20.2 °C / Exposure time: 0.995 sec. / Number of frames: 48 / Unit: 1/nm /
MinMax
Q0.0225 7.3176
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 930 /
MinMax
Q0.0778868 2.65281
P(R) point1 930
R0 19.7
Result
Type of curve: sec
Comments: The quoted experimental molecular mass, that corresponds to a 2:4 pUL7:pUL51 heterohexamer, was determined from SEC-MALLS-RI measurements performed in parallel to the SEC-SAXS. The SEC-SAXS ...Comments: The quoted experimental molecular mass, that corresponds to a 2:4 pUL7:pUL51 heterohexamer, was determined from SEC-MALLS-RI measurements performed in parallel to the SEC-SAXS. The SEC-SAXS data, Rg correlations through the dimer elution peak as well as the the SEC-MALLS-RI data are made available in the full-entry zip archive. The GASBORMX model displayed in this entry represents a 100 % volume fraction of the heterohexamer in solution that has been separated from a 1:2 pUL7:pUL51 heterotrimer (refer to SASBDB entry SASDG57).
ExperimentalPorod
MW168 kDa103.9 kDa
Volume-340 nm3

P(R)GuinierGuinier error
Forward scattering, I03136 3100.16 7.09
Radius of gyration, Rg4.788 nm4.56 nm0.28

MinMax
D-19.7
Guinier point21 95

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