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- SASDG37: 1:2 heterotrimer of pUL7 and pUL51(8-142) from herpes simplex virus 1 -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDG37
Sample1:2 heterotrimer of pUL7 and pUL51(8-142) from herpes simplex virus 1
  • Tegument protein UL7 (protein), pUL7, Human alphaherpesvirus 1 strain KOS
  • Tegument protein UL51 (protein), pUL51, Human alphaherpesvirus 1 strain KOS
Function / homologyHerpesvirus UL51 / Herpesvirus UL51 protein / Herpesvirus UL7-like / Herpesvirus UL7 like / viral tegument / host cell Golgi apparatus / UL7 / UL51
Function and homology information
Biological speciesHuman alphaherpesvirus 1 strain KOS
Contact author
  • Benjamin Butt (University of Cambridge)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3905
Type: dummy / Software: (SUPCOMB 23 (r9988)) / Radius of dummy atoms: 2.50 A / Symmetry: P1
Comment: Refined DAMMIN model of the HSV-1 pUL7:pUL51(8-142) 1:2 heterotrimer
Chi-square value: 1.040 / P-value: 0.100571
Model #3906
Type: dummy / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Representative GASBOR model of the HSV-1 pUL7:pUL51(8-142) 1:2 heterotrimer
Chi-square value: 1.03 / P-value: 0.161218
Model #3918
Type: mix / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Chi-square value: 1.034

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Sample

SampleName: 1:2 heterotrimer of pUL7 and pUL51(8-142) from herpes simplex virus 1
Specimen concentration: 4.5 mg/ml / Entity id: 1939 / 1945
BufferName: 20 mM tris, 200 mM NaCl, 3% (v/v) glycerol, 0.25 mM TCEP
pH: 7.5
Entity #1939Name: pUL7 / Type: protein / Description: Tegument protein UL7 / Formula weight: 33.907 / Num. of mol.: 1 / Source: Human alphaherpesvirus 1 strain KOS / References: UniProt: A0A110B4Q7
Sequence: MAAATADDEG SAATILKQAI AGDRSLVEAA EAISQQTLLR LACEVRQVGD RQPRFTATSI ARVDVAPGCR LRFVLDGSPE DAYVTSEDYF KRCCGQSSYR GFAVAVLTAN EDHVHSLAVP PLVLLHRFSL FNPRDLLDFE LACLLMYLEN CPRSHATPST FAKVLAWLGV ...Sequence:
MAAATADDEG SAATILKQAI AGDRSLVEAA EAISQQTLLR LACEVRQVGD RQPRFTATSI ARVDVAPGCR LRFVLDGSPE DAYVTSEDYF KRCCGQSSYR GFAVAVLTAN EDHVHSLAVP PLVLLHRFSL FNPRDLLDFE LACLLMYLEN CPRSHATPST FAKVLAWLGV AGRRTSPFER VRCLFLRSCH WVLNTLMFMV HVKPFDDEFV LPHWYMARYL LANNPPPVLS ALFCATPTSS SFRLPGPPPR SDCVAYNPAG IMGSCWASEE VRAPLVYWWL SETPKRQTSS LFYQFCGSLE VLFQ
Entity #1945Name: pUL51 / Type: protein / Description: Tegument protein UL51 / Formula weight: 14.814 / Num. of mol.: 2 / Source: Human alphaherpesvirus 1 strain KOS / References: UniProt: D3YPL0
Sequence:
MISGWGARPE EQYEMIRAAV PPSEAEPRLQ EALVVVNALL PAPITLDDAL GSLDDTRRLV KARALARTYH ACMVNLERLA RHHPGLEAPT IDGAVAAHQD KMRRLADTCM ATILQMYMSV GAADKSADVL VSQAIR

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3 mm
DetectorName: Pilatus 6M
Scan
Title: 1:2 heterotrimer of pUL7 and pUL51(8-142) from herpes simplex virus 1
Measurement date: May 16, 2019 / Cell temperature: 20.2 °C / Exposure time: 0.995 sec. / Number of frames: 3600 / Unit: 1/nm /
MinMax
Q0.0785 7.2987
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1450 /
MinMax
Q0.0785094 4.08388
P(R) point1 1450
R0 11.5
Result
Type of curve: single_conc
Comments: The quoted experimental molecular mass, that corresponds to a 1:2 pUL7:pUL51(8-142) heterotrimer, was determined from SEC-MALLS-RI measurements performed on identical samples, eluted in ...Comments: The quoted experimental molecular mass, that corresponds to a 1:2 pUL7:pUL51(8-142) heterotrimer, was determined from SEC-MALLS-RI measurements performed on identical samples, eluted in parallel to the SEC-SAXS. The SEC-SAXS data for each individual run, Rg correlations through the main elution peak as well as the the SEC-MALLS-RI data and hydrodynamic estimates obtained from QELS are made available in the full-entry zip archive. The CORAL rigid-body refined (atomistic) model displayed in this entry is one example of several alternatives whereby a second copy of pUL51 samples different spatial positions bound within the complex. Additional representations of the 1:2 pUL7:pUL51(8-142) heterotrimeric complex, including those incorporating mass spectrometry cross-linking distance constraints, are also made available in the full entry zip-archive.
ExperimentalPorod
MW67 kDa66.99 kDa
Volume-115.57 nm3

P(R)GuinierGuinier error
Forward scattering, I03346 3331.04 2.09
Radius of gyration, Rg3.023 nm2.96 nm0.01

MinMax
D-11.5
Guinier point9 131

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