SASDG35: Mothers against decapentaplegic homolog 2, S2MH1E3

基本情報

登録情報

データベース: SASBDB / ID: SASDG35

試料

Mothers against decapentaplegic homolog 2, S2MH1E3

• Mothers against decapentaplegic homolog 2 (protein), Homo sapiens

機能・相同性

分子機能:

regulation of binding / zygotic specification of dorsal/ventral axis / paraxial mesoderm morphogenesis / homomeric SMAD protein complex / activin responsive factor complex / SMAD4 MH2 Domain Mutants in Cancer / SMAD2/3 MH2 Domain Mutants in Cancer / nodal signaling pathway / SMAD protein complex / endoderm formation / heteromeric SMAD protein complex / co-SMAD binding / odontoblast differentiation / determination of left/right asymmetry in lateral mesoderm / FOXO-mediated transcription of cell cycle genes / regulation of transforming growth factor beta receptor signaling pathway / pericardium development / secondary palate development / trophoblast cell migration / SMAD2/3 Phosphorylation Motif Mutants in Cancer / TGFBR1 KD Mutants in Cancer / Transcriptional regulation of pluripotent stem cells / embryonic foregut morphogenesis / transforming growth factor beta receptor binding / Germ layer formation at gastrulation / primary miRNA processing / pulmonary valve morphogenesis / type I transforming growth factor beta receptor binding / Formation of definitive endoderm / SMAD protein signal transduction / Signaling by Activin / activin receptor signaling pathway / Formation of axial mesoderm / positive regulation of BMP signaling pathway / Signaling by NODAL / response to cholesterol / embryonic cranial skeleton morphogenesis / I-SMAD binding / pancreas development / aortic valve morphogenesis / signal transduction involved in regulation of gene expression / negative regulation of ossification / insulin secretion / anterior/posterior pattern specification / ureteric bud development / endocardial cushion morphogenesis / organ growth / adrenal gland development / SMAD binding / R-SMAD binding / TGF-beta receptor signaling activates SMADs / mesoderm formation / negative regulation of cell differentiation / anatomical structure morphogenesis / cell fate commitment / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / phosphatase binding / positive regulation of epithelial to mesenchymal transition / cis-regulatory region sequence-specific DNA binding / gastrulation / transforming growth factor beta receptor signaling pathway / Downregulation of TGF-beta receptor signaling / post-embryonic development / lung development / Downregulation of SMAD2/3:SMAD4 transcriptional activity / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / cellular response to glucose stimulus / tau protein binding / disordered domain specific binding / DNA-binding transcription activator activity, RNA polymerase II-specific / double-stranded DNA binding / DNA-binding transcription factor binding / transcription regulator complex / RNA polymerase II-specific DNA-binding transcription factor binding / in utero embryonic development / cell differentiation / DNA-binding transcription factor activity, RNA polymerase II-specific / cell population proliferation / Ub-specific processing proteases / intracellular signal transduction / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / negative regulation of DNA-templated transcription / intracellular membrane-bounded organelle / DNA-templated transcription / ubiquitin protein ligase binding / chromatin binding / positive regulation of gene expression / regulation of DNA-templated transcription / chromatin / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / protein-containing complex / nucleoplasm / metal ion binding / identical protein binding / nucleus / cytosol / cytoplasm

ドメイン・相同性:

MAD homology, MH1 / Dwarfin / SMAD MH1 domain superfamily / MAD homology domain 1 (MH1) profile. / SMAD domain, Dwarfin-type / MH2 domain / MAD homology domain 2 (MH2) profile. / Domain B in dwarfin family proteins / MAD homology 1, Dwarfin-type / MH1 domain / Domain A in dwarfin family proteins / SMAD-like domain superfamily / SMAD/FHA domain superfamily

構成要素:

Mothers against decapentaplegic homolog 2

• 生物種: Homo sapiens (ヒト)

登録者

• Tiago Lopes (IRB, Institute for Research in Biomedicine in Barcelona (IRB), Barcelona, Spain)

モデル

試料

• 試料: 名称: Mothers against decapentaplegic homolog 2, S2MH1E3 / 試料濃度: 1.00-5.00
• バッファ: 名称: 20 mM Tris 150 mM NaCl / pH: 7.2

要素 #1876

タイプ: protein / 記述: Mothers against decapentaplegic homolog 2 / 分子量: 19.014 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: Q15796
配列:

GAMGPVVKRL LGWKKSAGGS GGAGGGEQNG QEEKWCEKAV KSLVKKLKKT GRLDELEKAI TTQNCNTKCV TIPSTCSEIW GLSTPNTIDQ WDTTGLYSFS EQTRSLDGRL QVSHRKGLPH VIYCRLWRWP DLHSHHELKA IENCEYAFNL KKDEVCVNPY HYQRVETPV

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.099 Å
• 検出器: 名称: Pilatus 1M / タイプ: Dectris / Pixsize x: 172 mm

スキャン

タイトル: Mothers against decapentaplegic homolog 2, S2MH1E3
測定日: 2015年7月2日 / 保管温度: 10 °C / セル温度: 10 °C / 単位: 1/nm /

	Min	Max
Q	0.0609	4.9336

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 798 /

	Min	Max
Q	0.0703133	3.8189
P(R) point	1	798
R	0	7.4

結果

カーブのタイプ: merged /

	実験値	Porod
分子量	20.49 kDa	22 kDa
体積	-	35 nm3

	P(R)	Guinier	Guinier error
前方散乱 I0	20.85	20.78	0.017
慣性半径, Rg	1.93 nm	1.89 nm	0.01

	Min	Max
D	-	7.4
Guinier point	3	131