SASDG35: Mothers against decapentaplegic homolog 2, S2MH1E3

Basic information

Entry

Database: SASBDB / ID: SASDG35

Sample

Mothers against decapentaplegic homolog 2, S2MH1E3

• Mothers against decapentaplegic homolog 2 (protein), Homo sapiens

Function / homology

Function:

zygotic specification of dorsal/ventral axis / regulation of binding / homomeric SMAD protein complex / paraxial mesoderm morphogenesis / activin responsive factor complex / SMAD4 MH2 Domain Mutants in Cancer / SMAD2/3 MH2 Domain Mutants in Cancer / nodal signaling pathway / SMAD protein complex / endoderm formation / co-SMAD binding / heteromeric SMAD protein complex / odontoblast differentiation / determination of left/right asymmetry in lateral mesoderm / secondary palate development / pericardium development / FOXO-mediated transcription of cell cycle genes / SMAD2/3 Phosphorylation Motif Mutants in Cancer / TGFBR1 KD Mutants in Cancer / Transcriptional regulation of pluripotent stem cells / regulation of transforming growth factor beta receptor signaling pathway / embryonic foregut morphogenesis / transforming growth factor beta receptor binding / primary miRNA processing / Germ layer formation at gastrulation / pulmonary valve morphogenesis / signal transduction involved in regulation of gene expression / Formation of definitive endoderm / type I transforming growth factor beta receptor binding / activin receptor signaling pathway / Signaling by Activin / Formation of axial mesoderm / SMAD protein signal transduction / positive regulation of BMP signaling pathway / Signaling by NODAL / embryonic cranial skeleton morphogenesis / response to cholesterol / I-SMAD binding / pancreas development / aortic valve morphogenesis / insulin secretion / ureteric bud development / anterior/posterior pattern specification / endocardial cushion morphogenesis / organ growth / adrenal gland development / TGF-beta receptor signaling activates SMADs / SMAD binding / R-SMAD binding / mesoderm formation / gastrulation / anatomical structure morphogenesis / positive regulation of epithelial to mesenchymal transition / phosphatase binding / cell fate commitment / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / cis-regulatory region sequence-specific DNA binding / Downregulation of TGF-beta receptor signaling / post-embryonic development / transforming growth factor beta receptor signaling pathway / cellular response to glucose stimulus / Downregulation of SMAD2/3:SMAD4 transcriptional activity / lung development / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / tau protein binding / disordered domain specific binding / double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific / DNA-binding transcription factor binding / in utero embryonic development / transcription regulator complex / RNA polymerase II-specific DNA-binding transcription factor binding / cell differentiation / DNA-binding transcription factor activity, RNA polymerase II-specific / Ub-specific processing proteases / intracellular signal transduction / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / negative regulation of DNA-templated transcription / DNA-templated transcription / ubiquitin protein ligase binding / chromatin binding / chromatin / positive regulation of gene expression / regulation of DNA-templated transcription / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / protein-containing complex / nucleoplasm / identical protein binding / metal ion binding / nucleus / cytosol / cytoplasm

Domain/homology:

MAD homology, MH1 / Dwarfin / SMAD MH1 domain superfamily / MAD homology domain 1 (MH1) profile. / SMAD domain, Dwarfin-type / MH2 domain / MAD homology domain 2 (MH2) profile. / Domain B in dwarfin family proteins / MAD homology 1, Dwarfin-type / MH1 domain / Domain A in dwarfin family proteins / SMAD-like domain superfamily / SMAD/FHA domain superfamily

Component:

Mothers against decapentaplegic homolog 2

• Biological species: Homo sapiens (human)

Contact author

• Tiago Lopes (IRB, Institute for Research in Biomedicine in Barcelona (IRB), Barcelona, Spain)

Models

Sample

• Sample: Name: Mothers against decapentaplegic homolog 2, S2MH1E3 / Specimen concentration: 1.00-5.00
• Buffer: Name: 20 mM Tris 150 mM NaCl / pH: 7.2

Entity #1876

Type: protein / Description: Mothers against decapentaplegic homolog 2 / Formula weight: 19.014 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q15796
Sequence:

GAMGPVVKRL LGWKKSAGGS GGAGGGEQNG QEEKWCEKAV KSLVKKLKKT GRLDELEKAI TTQNCNTKCV TIPSTCSEIW GLSTPNTIDQ WDTTGLYSFS EQTRSLDGRL QVSHRKGLPH VIYCRLWRWP DLHSHHELKA IENCEYAFNL KKDEVCVNPY HYQRVETPV

Experimental information

• Beam: Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.099 Å
• Detector: Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm

Scan

Title: Mothers against decapentaplegic homolog 2, S2MH1E3 / Measurement date: Jul 2, 2015 / Storage temperature: 10 °C / Cell temperature: 10 °C / Unit: 1/nm /

	Min	Max
Q	0.0609	4.9336

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 798 /

	Min	Max
Q	0.0703133	3.8189
P(R) point	1	798
R	0	7.4

Result

Type of curve: merged /

	Experimental	Porod
MW	20.49 kDa	22 kDa
Volume	-	35 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	20.85	20.78	0.017
Radius of gyration, Rg	1.93 nm	1.89 nm	0.01

	Min	Max
D	-	7.4
Guinier point	3	131