SASDG29: Truncated Apolipoprotein E4 (1-191) bound to 4 µM Suramin

基本情報

登録情報

データベース: SASBDB / ID: SASDG29

試料

Truncated Apolipoprotein E4 (1-191) bound to 4 µM Suramin

• Apolipoprotein E4 (1-191) (protein), ApoE4, Homo sapiens
• Suraminスラミン (other)

機能・相同性

分子機能:

chylomicron remnant / lipid transport involved in lipid storage / triglyceride-rich lipoprotein particle clearance / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / positive regulation of heparan sulfate proteoglycan binding / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / discoidal high-density lipoprotein particle / chylomicron remnant clearance / リポタンパク質 / maintenance of location in cell / very-low-density lipoprotein particle clearance / intermediate-density lipoprotein particle / very-low-density lipoprotein particle remodeling / regulation of amyloid-beta clearance / response to caloric restriction / Chylomicron clearance / negative regulation of triglyceride metabolic process / NMDA glutamate receptor clustering / Chylomicron remodeling / phosphatidylcholine-sterol O-acyltransferase activator activity / lipid transporter activity / positive regulation of phospholipid efflux / カイロミクロン / Chylomicron assembly / positive regulation of low-density lipoprotein particle receptor catabolic process / positive regulation of cholesterol metabolic process / regulation of behavioral fear response / regulation of protein metabolic process / high-density lipoprotein particle clearance / multivesicular body, internal vesicle / lipoprotein catabolic process / very-low-density lipoprotein particle receptor binding / regulation of amyloid fibril formation / melanosome organization / high-density lipoprotein particle remodeling / phospholipid efflux / low-density lipoprotein particle / AMPA glutamate receptor clustering / cholesterol transfer activity / positive regulation by host of viral process / reverse cholesterol transport / positive regulation of amyloid-beta clearance / high-density lipoprotein particle assembly / very-low-density lipoprotein particle / positive regulation of CoA-transferase activity / lipoprotein biosynthetic process / protein import / negative regulation of blood coagulation / high-density lipoprotein particle / low-density lipoprotein particle remodeling / negative regulation of amyloid fibril formation / synaptic transmission, cholinergic / negative regulation of cholesterol biosynthetic process / heparan sulfate proteoglycan binding / regulation of Cdc42 protein signal transduction / amyloid precursor protein metabolic process / triglyceride homeostasis / regulation of amyloid precursor protein catabolic process / positive regulation of membrane protein ectodomain proteolysis / HDL remodeling / negative regulation of endothelial cell migration / Scavenging by Class A Receptors / cholesterol efflux / negative regulation of protein metabolic process / artery morphogenesis / regulation of axon extension / regulation of cholesterol metabolic process / low-density lipoprotein particle receptor binding / positive regulation of amyloid fibril formation / triglyceride metabolic process / positive regulation of dendritic spine development / virion assembly / regulation of innate immune response / regulation of neuronal synaptic plasticity / locomotory exploration behavior / negative regulation of amyloid-beta formation / lipoprotein particle binding / negative regulation of endothelial cell proliferation / positive regulation of endocytosis / antioxidant activity / cGMP-mediated signaling / cholesterol catabolic process / response to dietary excess / negative regulation of blood vessel endothelial cell migration / negative regulation of long-term synaptic potentiation / positive regulation of dendritic spine maintenance / negative regulation of platelet activation / positive regulation of cholesterol efflux / intracellular transport / regulation of protein-containing complex assembly / negative regulation of protein secretion / fatty acid homeostasis / long-term memory / long-chain fatty acid transport / positive regulation of lipid biosynthetic process / synaptic cleft / regulation of proteasomal protein catabolic process

ドメイン・相同性:

Apolipoprotein A/E / Apolipoprotein A1/A4/E domain

構成要素:

アポリポプロテインE

• 生物種: Homo sapiens (ヒト)

登録者

• Lucas Kraft (University of Sussex, Brighton, UK)

モデル

試料

• 試料: 名称: Truncated Apolipoprotein E4 (1-191) bound to 4 µM Suramin; 試料濃度: 4 mg/ml / Entity id: 1635 / 1682
• バッファ: 名称: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

要素 #1635

名称: ApoE4 / タイプ: protein / 記述: Apolipoprotein E4 (1-191) / 分子量: 22.605 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P02649
配列:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVRGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KRLAVYQAGA REGAERGLSA IRERLGPLVE QGRVR

要素 #1682

タイプ: other / 記述: Suraminスラミン / 分子量: 1.297 / 分子数: 1
配列:

C51H40N6O2 3S6

実験情報

• ビーム: 設備名称: Diamond Light Source B21 / 地域: Didcot / 国: UK / 形状: 1 x 5 mm / 線源: X-ray synchrotronシンクロトロン / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 4.036 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Truncated Apolipoprotein E4 (1-191) bound to 4 µM Suramin
測定日: 2018年5月3日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 28 / 単位: 1/A /

	Min	Max
Q	0.003	0.37

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 1387 /

	Min	Max
Q	0.0167987	0.325266
P(R) point	1	1387
R	0	78

結果

Experimental MW: 24 kDa / カーブのタイプ: single_conc / コメント: ApoE4 (1-191) + 4 µM Suramin

	P(R)	Guinier	Guinier error
前方散乱 I0	0.07988	0.081	0.00052
慣性半径, Rg	2.43 nm	2.433 nm	0.027

	Min	Max
D	-	7.8
Guinier point	63	216