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- SASDFZ9: RuvB-like 1 and 2, sperm-associated antigen 1 complex (His-RuvBL1... -

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Basic information

Entry
Database: SASBDB / ID: SASDFZ9
SampleRuvB-like 1 and 2, sperm-associated antigen 1 complex (His-RuvBL1/RuvBL2/SPAG1)
  • RuvB-like 1 (protein), Homo sapiens
  • RuvB-like 2 (protein), Homo sapiens
  • Sperm-associated antigen 1 (protein), SPAG1, Homo sapiens
Function / homology
Function and homology information


axonemal dynein complex assembly / promoter-enhancer loop anchoring activity / telomerase RNA localization to Cajal body / regulation of DNA strand elongation / positive regulation of telomere maintenance in response to DNA damage / establishment of protein localization to chromatin / R2TP complex / dynein axonemal particle / Swr1 complex / RPAP3/R2TP/prefoldin-like complex ...axonemal dynein complex assembly / promoter-enhancer loop anchoring activity / telomerase RNA localization to Cajal body / regulation of DNA strand elongation / positive regulation of telomere maintenance in response to DNA damage / establishment of protein localization to chromatin / R2TP complex / dynein axonemal particle / Swr1 complex / RPAP3/R2TP/prefoldin-like complex / Ino80 complex / regulation of double-strand break repair / box C/D snoRNP assembly / protein folding chaperone complex / regulation of chromosome organization / NuA4 histone acetyltransferase complex / TFIID-class transcription factor complex binding / regulation of DNA replication / MLL1 complex / regulation of embryonic development / Telomere Extension By Telomerase / single fertilization / RNA polymerase II core promoter sequence-specific DNA binding / regulation of DNA repair / positive regulation of double-strand break repair via homologous recombination / Deposition of new CENPA-containing nucleosomes at the centromere / telomere maintenance / DNA helicase activity / TBP-class protein binding / positive regulation of DNA repair / cellular response to estradiol stimulus / Formation of the beta-catenin:TCF transactivating complex / euchromatin / negative regulation of canonical Wnt signaling pathway / DNA Damage Recognition in GG-NER / ADP binding / beta-catenin binding / chromatin DNA binding / nuclear matrix / cellular response to UV / UCH proteinases / transcription corepressor activity / unfolded protein binding / positive regulation of canonical Wnt signaling pathway / protein folding / nucleosome / HATs acetylate histones / ATPase binding / spermatogenesis / regulation of apoptotic process / DNA recombination / DNA helicase / transcription coactivator activity / hydrolase activity / regulation of cell cycle / Ub-specific processing proteases / protein stabilization / nuclear speck / cilium / ciliary basal body / cadherin binding / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin remodeling / ribonucleoprotein complex / cell division / DNA repair / centrosome / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / GTP binding / positive regulation of DNA-templated transcription / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / ATP hydrolysis activity / extracellular exosome / nucleoplasm / ATP binding / identical protein binding / nucleus / membrane / cytoplasm / cytosol
Similarity search - Function
: / RNA-polymerase II-associated protein 3-like, C-terminal domain / Potential Monad-binding region of RPAP3 / RuvB-like / RuvB-like, AAA-lid domain / RuvBL1/2, DNA/RNA binding domain / TIP49 P-loop domain / TIP49 AAA-lid domain / TIP49, P-loop domain / Tetratricopeptide repeat ...: / RNA-polymerase II-associated protein 3-like, C-terminal domain / Potential Monad-binding region of RPAP3 / RuvB-like / RuvB-like, AAA-lid domain / RuvBL1/2, DNA/RNA binding domain / TIP49 P-loop domain / TIP49 AAA-lid domain / TIP49, P-loop domain / Tetratricopeptide repeat / Tetratricopeptide repeat / TPR repeat region circular profile. / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Tetratricopeptide-like helical domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Sperm-associated antigen 1 / RuvB-like 2 / RuvB-like 1
Similarity search - Component
Biological speciesHomo sapiens (human)
Contact author
  • Raphael Dos Santos Morais (University of Lorraine, France)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2938
Type: dummy / Radius of dummy atoms: 3.90 A / Chi-square value: 1.878

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Sample

SampleName: RuvB-like 1 and 2, sperm-associated antigen 1 complex (His-RuvBL1/RuvBL2/SPAG1)
Specimen concentration: 7 mg/ml / Entity id: 1608 / 1609 / 1792
BufferName: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP / pH: 7.5
Entity #1608Type: protein / Description: RuvB-like 1 / Formula weight: 51.759 / Num. of mol.: 6 / Source: Homo sapiens / References: UniProt: Q9Y265
Sequence: MVHHHHHHLV PRGSKIEEVK STTKTQRIAS HSHVKGLGLD ESGLAKQAAS GLVGQENARE ACGVIVELIK SKKMAGRAVL LAGPPGTGKT ALALAIAQEL GSKVPFCPMV GSEVYSTEIK KTEVLMENFR RAIGLRIKET KEVYEGEVTE LTPCETENPM GGYGKTISHV ...Sequence:
MVHHHHHHLV PRGSKIEEVK STTKTQRIAS HSHVKGLGLD ESGLAKQAAS GLVGQENARE ACGVIVELIK SKKMAGRAVL LAGPPGTGKT ALALAIAQEL GSKVPFCPMV GSEVYSTEIK KTEVLMENFR RAIGLRIKET KEVYEGEVTE LTPCETENPM GGYGKTISHV IIGLKTAKGT KQLKLDPSIF ESLQKERVEA GDVIYIEANS GAVKRQGRCD TYATEFDLEA EEYVPLPKGD VHKKKEIIQD VTLHDLDVAN ARPQGGQDIL SMMGQLMKPK KTEITDKLRG EINKVVNKYI DQGIAELVPG VLFVDEVHML DIECFTYLHR ALESSIAPIV IFASNRGNCV IRGTEDITSP HGIPLDLLDR VMIIRTMLYT PQEMKQIIKI RAQTEGINIS EEALNHLGEI GTKTTLRYSV QLLTPANLLA KINGKDSIEK EHVEEISELF YDAKSSAKIL ADQQDKYMK
Entity #1609Type: protein / Description: RuvB-like 2 / Formula weight: 51.886 / Num. of mol.: 6 / Source: Homo sapiens / References: UniProt: Q9Y230
Sequence: MATVTATTKV PEIRDVTRIE RIGAHSHIRG LGLDDALEPR QASQGMVGQL AARRAAGVVL EMIREGKIAG RAVLIAGQPG TGKTAIAMGM AQALGPDTPF TAIAGSEIFS LEMSKTEALT QAFRRSIGVR IKEETEIIEG EVVEIQIDRP ATGTGSKVGK LTLKTTEMET ...Sequence:
MATVTATTKV PEIRDVTRIE RIGAHSHIRG LGLDDALEPR QASQGMVGQL AARRAAGVVL EMIREGKIAG RAVLIAGQPG TGKTAIAMGM AQALGPDTPF TAIAGSEIFS LEMSKTEALT QAFRRSIGVR IKEETEIIEG EVVEIQIDRP ATGTGSKVGK LTLKTTEMET IYDLGTKMIE SLTKDKVQAG DVITIDKATG KISKLGRSFT RARDYDAMGS QTKFVQCPDG ELQKRKEVVH TVSLHEIDVI NSRTQGFLAL FSGDTGEIKS EVREQINAKV AEWREEGKAE IIPGVLFIDE VHMLDIESFS FLNRALESDM APVLIMATNR GITRIRGTSY QSPHGIPIDL LDRLLIVSTT PYSEKDTKQI LRIRCEEEDV EMSEDAYTVL TRIGLETSLR YAIQLITAAS LVCRKRKGTE VQVDDIKRVY SLFLDESRST QYMKEYQDAF LFNELKGETM DTSLEVLFQ
Entity #1792Name: SPAG1 / Type: protein / Description: Sperm-associated antigen 1 / Formula weight: 103.929 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q07617
Sequence: GPHMTTKDYP SLWGFGTTKT FKIPIEHLDF KYIEKCSDVK HLEKILCVLR SGEEGYYPEL TEFCEKHLQA LAPESRALRK DKPAATAASF TAEEWEKIDG DIKSWVSEIK KEEDKMHFHE TETFPAMKDN LPPVRGSNSC LHVGKEKYSK RPTKKKTPRD YAEWDKFDVE ...Sequence:
GPHMTTKDYP SLWGFGTTKT FKIPIEHLDF KYIEKCSDVK HLEKILCVLR SGEEGYYPEL TEFCEKHLQA LAPESRALRK DKPAATAASF TAEEWEKIDG DIKSWVSEIK KEEDKMHFHE TETFPAMKDN LPPVRGSNSC LHVGKEKYSK RPTKKKTPRD YAEWDKFDVE KECLKIDEDY KEKTVIDKSH LSKIETRIDT AGLTEKEKDF LATREKEKGN EAFNSGDYEE AVMYYTRSIS ALPTVVAYNN RAQAEIKLQN WNSAFQDCEK VLELEPGNVK ALLRRATTYK HQNKLREATE DLSKVLDVEP DNDLAKKTLS EVERDLKNSE AASETQTKGK RMVIQEIENS EDEEGKSGRK HEDGGGDKKP AEPAGAARAA QPCVMGNIQK KLTGKAEGGK RPARGAPQRG QTPEAGADKR SPRRASAAAA AGGGATGHPG GGQGAENPAG LKSQGNELFR SGQFAEAAGK YSAAIALLEP AGSEIADDLS ILYSNRAACY LKEGNCSGCI QDCNRALELH PFSMKPLLRR AMAYETLEQY GKAYVDYKTV LQIDCGLQLA NDSVNRLSRI LMELDGPNWR EKLSPIPAVP ASVPLQAWHP AKEMISKQAG DSSSHRQQGI TDEKTFKALK EEGNQCVNDK NYKDALSKYS ECLKINNKEC AIYTNRALCY LKLCQFEEAK QDCDQALQLA DGNVKAFYRR ALAHKGLKNY QKSLIDLNKV ILLDPSIIEA KMELEEVTRL LNLKDKTAPF NKEKERRKIE IQEVNEGKEE PGRPAGEVSM GCLASEKGGK SSRSPEDPEK LPIAKPNNAY EFGQIINALS TRKDKEACAH LLAITAPKDL PMFLSNKLEG DTFLLLIQSL KNNLIEKDPS LVYQHLLYLS KAERFKMMLT LISKGQKELI EQLFEDLSDT PNNHFTLEDI QALKRQYEL

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Experimental information

BeamInstrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 2 mm
DetectorName: Eiger 4M / Pixsize x: 75 mm
Scan
Title: RuvB-like 1 and 2, sperm-associated antigen 1 complex (His-RuvBL1/RuvBL2/SPAG1)
Measurement date: Mar 21, 2019 / Cell temperature: 15 °C / Exposure time: 1 sec. / Number of frames: 26 / Unit: 1/A /
MinMax
Q0.0041 0.5786
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 248 /
MinMax
Q0.00592921 0.118567
P(R) point1 248
R0 348
Result
Type of curve: sec /
ExperimentalPorod
MW807 kDa-
Volume-1800 nm3

P(R)GuinierGuinier error
Forward scattering, I00.05402 0.05335 -
Radius of gyration, Rg7.2 nm6.805 nm0.02

MinMax
D-34.8
Guinier point5 33

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