SASDFZ8: Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin

基本情報

登録情報

データベース: SASBDB / ID: SASDFZ8

試料

Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin

• Apolipoprotein E2 (protein), ApoE2, Homo sapiens
• Heparin (other)

機能・相同性

分子機能:

positive regulation of heparan sulfate proteoglycan binding / lipid transport involved in lipid storage / chylomicron remnant / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / regulation of cellular response to very-low-density lipoprotein particle stimulus / positive regulation of low-density lipoprotein particle receptor catabolic process / metal chelating activity / triglyceride-rich lipoprotein particle clearance / lipoprotein particle / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / regulation of amyloid-beta clearance / negative regulation of cholesterol biosynthetic process / discoidal high-density lipoprotein particle / intermediate-density lipoprotein particle / chylomicron remnant clearance / maintenance of location in cell / very-low-density lipoprotein particle remodeling / very-low-density lipoprotein particle receptor binding / very-low-density lipoprotein particle clearance / Chylomicron clearance / NMDA glutamate receptor clustering / acylglycerol homeostasis / response to caloric restriction / Chylomicron remodeling / cellular response to lipoprotein particle stimulus / negative regulation of triglyceride metabolic process / phosphatidylcholine-sterol O-acyltransferase activator activity / positive regulation of phospholipid efflux / Chylomicron assembly / positive regulation of cholesterol metabolic process / regulation of amyloid fibril formation / regulation of behavioral fear response / regulation of protein metabolic process / high-density lipoprotein particle clearance / chylomicron / lipoprotein catabolic process / lipid transporter activity / high-density lipoprotein particle remodeling / phospholipid efflux / melanosome organization / multivesicular body, internal vesicle / AMPA glutamate receptor clustering / cholesterol transfer activity / reverse cholesterol transport / high-density lipoprotein particle assembly / positive regulation of amyloid-beta clearance / very-low-density lipoprotein particle / positive regulation by host of viral process / low-density lipoprotein particle / lipoprotein biosynthetic process / positive regulation of CoA-transferase activity / protein import / high-density lipoprotein particle / negative regulation of blood coagulation / low-density lipoprotein particle remodeling / negative regulation of amyloid fibril formation / heparan sulfate proteoglycan binding / synaptic transmission, cholinergic / regulation of Cdc42 protein signal transduction / amyloid precursor protein metabolic process / regulation of amyloid precursor protein catabolic process / cholesterol catabolic process / triglyceride homeostasis / positive regulation of membrane protein ectodomain proteolysis / negative regulation of protein metabolic process / HDL remodeling / triglyceride metabolic process / negative regulation of endothelial cell migration / Scavenging by Class A Receptors / cholesterol efflux / low-density lipoprotein particle receptor binding / artery morphogenesis / regulation of axon extension / regulation of cholesterol metabolic process / positive regulation of amyloid fibril formation / virion assembly / positive regulation of dendritic spine development / regulation of innate immune response / negative regulation of amyloid-beta formation / negative regulation of endothelial cell proliferation / locomotory exploration behavior / regulation of neuronal synaptic plasticity / antioxidant activity / lipoprotein particle binding / positive regulation of endocytosis / response to dietary excess / negative regulation of blood vessel endothelial cell migration / negative regulation of long-term synaptic potentiation / negative regulation of protein secretion / positive regulation of dendritic spine maintenance / negative regulation of platelet activation / positive regulation of cholesterol efflux / regulation of protein-containing complex assembly / intracellular transport / fatty acid homeostasis / long-term memory / long-chain fatty acid transport / positive regulation of lipid biosynthetic process / regulation of proteasomal protein catabolic process

ドメイン・相同性:

Apolipoprotein A/E / : / Apolipoprotein A1/A4/E domain

構成要素:

Apolipoprotein E

• 生物種: Homo sapiens (ヒト)

登録者

• Lucas Kraft (University of Sussex, Brighton, UK)

モデル

試料

• 試料: 名称: Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin; 試料濃度: 4 mg/ml / Entity id: 1086 / 1633
• バッファ: 名称: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

要素 #1086

名称: ApoE2 / タイプ: protein / 記述: Apolipoprotein E2 / 分子量: 34.606 / 分子数: 4 / 由来: Homo sapiens / 参照: UniProt: P02649
配列:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVCGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KCLAVYQAGA REGAERGLSA IRERLGPLVE QGRVRAATVG SLAGQPLQER AQAWGERLRA RMEEMGSRTR DRLDEVKEQV AEVRAKLEEQ AQQIRLQAEA FQARLKSWFE PLVEDMQRQW AGLVEKVQAA VGTSAAPVPS DNH

要素 #1633

タイプ: other / 記述: Heparin / 分子量: 15 / 分子数: 1
配列:

(C12H19NO20S3)n

実験情報

• ビーム: 設備名称: Diamond Light Source B21 / 地域: Didcot / 国: UK / 形状: 1 x 5 mm / 線源: X-ray synchrotron / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 4.036 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin
測定日: 2018年5月3日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 28 / 単位: 1/A /

	Min	Max
Q	0.003	0.37

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 980 /

	Min	Max
Q	0.00745118	0.225337
P(R) point	1	980
R	0	245

結果

カーブのタイプ: single_conc
コメント: ApoE2 + 330 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.

	実験値	Porod
分子量	303 kDa	-
体積	-	696 nm3

	P(R)	Guinier	Guinier error
前方散乱 I0	0.7629	0.766	0.0056
慣性半径, Rg	7.34 nm	7.217 nm	0.086

	Min	Max
D	-	24.5
Guinier point	22	62