SASDFZ8: Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin

Basic information

Entry

Database: SASBDB / ID: SASDFZ8

Sample

Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin

• Apolipoprotein E2 (protein), ApoE2, Homo sapiens
• Heparin (other)

Function / homology

Function:

chylomicron remnant / lipid transport involved in lipid storage / triglyceride-rich lipoprotein particle clearance / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / positive regulation of heparan sulfate proteoglycan binding / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / discoidal high-density lipoprotein particle / chylomicron remnant clearance / lipoprotein particle / maintenance of location in cell / very-low-density lipoprotein particle clearance / intermediate-density lipoprotein particle / very-low-density lipoprotein particle remodeling / regulation of amyloid-beta clearance / response to caloric restriction / Chylomicron clearance / negative regulation of triglyceride metabolic process / NMDA glutamate receptor clustering / chylomicron / Chylomicron remodeling / phosphatidylcholine-sterol O-acyltransferase activator activity / positive regulation of phospholipid efflux / Chylomicron assembly / lipid transporter activity / positive regulation of low-density lipoprotein particle receptor catabolic process / positive regulation of cholesterol metabolic process / regulation of behavioral fear response / high-density lipoprotein particle remodeling / high-density lipoprotein particle clearance / multivesicular body, internal vesicle / lipoprotein catabolic process / very-low-density lipoprotein particle receptor binding / phospholipid efflux / regulation of amyloid fibril formation / regulation of protein metabolic process / AMPA glutamate receptor clustering / low-density lipoprotein particle / positive regulation by host of viral process / cholesterol transfer activity / reverse cholesterol transport / positive regulation of amyloid-beta clearance / high-density lipoprotein particle assembly / very-low-density lipoprotein particle / positive regulation of CoA-transferase activity / melanosome organization / lipoprotein biosynthetic process / protein import / negative regulation of blood coagulation / low-density lipoprotein particle remodeling / high-density lipoprotein particle / negative regulation of amyloid fibril formation / negative regulation of cholesterol biosynthetic process / amyloid precursor protein metabolic process / heparan sulfate proteoglycan binding / regulation of Cdc42 protein signal transduction / triglyceride homeostasis / regulation of amyloid precursor protein catabolic process / cholesterol catabolic process / synaptic transmission, cholinergic / positive regulation of membrane protein ectodomain proteolysis / HDL remodeling / negative regulation of endothelial cell migration / cholesterol efflux / Scavenging by Class A Receptors / negative regulation of protein metabolic process / artery morphogenesis / triglyceride metabolic process / regulation of axon extension / regulation of cholesterol metabolic process / positive regulation of amyloid fibril formation / low-density lipoprotein particle receptor binding / positive regulation of dendritic spine development / virion assembly / regulation of innate immune response / regulation of neuronal synaptic plasticity / locomotory exploration behavior / lipoprotein particle binding / positive regulation of endocytosis / negative regulation of amyloid-beta formation / negative regulation of endothelial cell proliferation / antioxidant activity / cGMP-mediated signaling / response to dietary excess / negative regulation of blood vessel endothelial cell migration / negative regulation of long-term synaptic potentiation / negative regulation of platelet activation / positive regulation of cholesterol efflux / positive regulation of dendritic spine maintenance / intracellular transport / regulation of protein-containing complex assembly / negative regulation of protein secretion / long-term memory / fatty acid homeostasis / long-chain fatty acid transport / positive regulation of lipid biosynthetic process / synaptic cleft / regulation of proteasomal protein catabolic process

Domain/homology:

Apolipoprotein A/E / Apolipoprotein A1/A4/E domain

Component:

Apolipoprotein E

• Biological species: Homo sapiens (human)

Contact author

• Lucas Kraft (University of Sussex, Brighton, UK)

Models

Sample

• Sample: Name: Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin / Specimen concentration: 4 mg/ml / Entity id: 1086 / 1633
• Buffer: Name: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

Entity #1086

Name: ApoE2 / Type: protein / Description: Apolipoprotein E2 / Formula weight: 34.606 / Num. of mol.: 4 / Source: Homo sapiens / References: UniProt: P02649
Sequence:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVCGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KCLAVYQAGA REGAERGLSA IRERLGPLVE QGRVRAATVG SLAGQPLQER AQAWGERLRA RMEEMGSRTR DRLDEVKEQV AEVRAKLEEQ AQQIRLQAEA FQARLKSWFE PLVEDMQRQW AGLVEKVQAA VGTSAAPVPS DNH

Entity #1633

Type: other / Description: Heparin / Formula weight: 15 / Num. of mol.: 1
Sequence:

(C12H19NO20S3)n

Experimental information

• Beam: Instrument name: Diamond Light Source B21 / City: Didcot / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.036 mm
• Detector: Name: Pilatus 2M

Scan

Title: Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin
Measurement date: May 3, 2018 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 28 / Unit: 1/A /

	Min	Max
Q	0.003	0.37

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 980 /

	Min	Max
Q	0.00745118	0.225337
P(R) point	1	980
R	0	245

Result

Type of curve: single_conc
Comments: ApoE2 + 330 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.

	Experimental	Porod
MW	303 kDa	-
Volume	-	696 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.7629	0.766	0.0056
Radius of gyration, Rg	7.34 nm	7.217 nm	0.086

	Min	Max
D	-	24.5
Guinier point	22	62