SASDFY6: DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80...

Basic information

• Entry: Database: SASBDB / ID: SASDFY6

Sample

DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:8)

• 80bp_DNA Forward (DNA), Escherichia coli
• 80bp_DNA Reverse (DNA), Escherichia coli K-12
• DNA-binding protein HU-alpha, E38K/V42L double mutant (protein), Escherichia coli K-12

Function / homology

Function:

HU-DNA complex / DnaA-HU complex / bacterial nucleoid DNA packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding / identical protein binding / membrane / cytosol

Domain/homology:

Histone-like DNA-binding protein, conserved site / Bacterial histone-like DNA-binding proteins signature. / Histone-like DNA-binding protein / Bacterial DNA-binding protein / bacterial (prokaryotic) histone like domain / Integration host factor (IHF)-like DNA-binding domain superfamily

Component:

DNA-binding protein HU-alpha

• Biological species: Escherichia coli (E. coli); Escherichia coli K-12 (bacteria)

Contact author

• Soumya G Remesh (Lawrence Berkeley National laboratory)

Models

• Model #3029: ; Type: atomic / Chi-square value: 0.797437547263

Sample

• Sample: Name: DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:8); Entity id: 1622 / 1623 / 1659
• Buffer: Name: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF / pH: 7.5

Entity #1622

Type: DNA / Description: 80bp_DNA Forward / Formula weight: 24.849 / Num. of mol.: 1 / Source: Escherichia coli
Sequence:

AATGAGGTAA CAACGAAAGC AGATGATAGC TGCTTATCAA TTTGTTGCAA ACAAGTAGCC GCGCCCAATG AGGTAACAAT

Entity #1623

Type: DNA / Description: 80bp_DNA Reverse / Formula weight: 24.612 / Num. of mol.: 1 / Source: Escherichia coli K-12
Sequence:

TTACTCCATT GTTGCTTTCG TCTACTATCG ACGAATAGTT AAACAACGTT TGTTCATCGG CGCGGGTTAC TCCATTGTTA

Entity #1659

Type: protein
Description: DNA-binding protein HU-alpha, E38K/V42L double mutant
Formula weight: 9.548 / Num. of mol.: 8 / Source: Escherichia coli K-12 / References: UniProt: P0ACF0
Sequence:

MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITESLKKGD ALQLVGFGTF KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK

Experimental information

• Beam: Instrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS); City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 1.5 mm
• Detector: Name: MAR 165 CCD

Scan

Title: DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:8)
Measurement date: Apr 23, 2015 / Cell temperature: 10 °C / Exposure time: 0.1 sec. / Number of frames: 32 / Unit: 1/A /

	Min	Max
Q	0.01	0.325

Distance distribution function P(R)

Sofotware P(R): GNOM 4.6 / Number of points: 258 /

	Min	Max
Q	0.01216	0.3247
P(R) point	3	260
R	0	280.5

Result

Type of curve: single_conc /

	Experimental	Porod
MW	140 kDa	-
Volume	-	296 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	536	462.76	6.01
Radius of gyration, Rg	7.89 nm	5.77 nm	0.317

	Min	Max
D	-	28.05
Guinier point	2	21