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- SASDFY4: R4-15 human dystrophin fragment (Human dystrophin central domain ... -

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Basic information

Entry
Database: SASBDB / ID: SASDFY4
SampleR4-15 human dystrophin fragment
  • Human dystrophin central domain R4-15 fragment (protein)
Function / homology
Function and homology information


regulation of muscle system process / regulation of cellular response to growth factor stimulus / regulation of skeletal muscle contraction / syntrophin complex / synaptic signaling / cardiac muscle cell action potential / regulation of voltage-gated calcium channel activity / negative regulation of peptidyl-cysteine S-nitrosylation / positive regulation of sodium ion transmembrane transporter activity / dystrophin-associated glycoprotein complex ...regulation of muscle system process / regulation of cellular response to growth factor stimulus / regulation of skeletal muscle contraction / syntrophin complex / synaptic signaling / cardiac muscle cell action potential / regulation of voltage-gated calcium channel activity / negative regulation of peptidyl-cysteine S-nitrosylation / positive regulation of sodium ion transmembrane transporter activity / dystrophin-associated glycoprotein complex / regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion / peptide biosynthetic process / cell-substrate junction / motile cilium assembly / dystroglycan binding / vinculin binding / muscle cell development / costamere / neuron projection terminus / Striated Muscle Contraction / filopodium membrane / muscle organ development / structural constituent of muscle / muscle cell cellular homeostasis / maintenance of blood-brain barrier / myosin binding / nitric-oxide synthase binding / Non-integrin membrane-ECM interactions / neuron development / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / negative regulation of peptidyl-serine phosphorylation / regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / skeletal muscle tissue development / cardiac muscle contraction / regulation of ryanodine-sensitive calcium-release channel activity / response to muscle stretch / positive regulation of neuron differentiation / regulation of heart rate / filopodium / sarcolemma / protein localization / structural constituent of cytoskeleton / Z disc / positive regulation of neuron projection development / actin binding / postsynaptic membrane / protein-containing complex assembly / cytoskeleton / membrane raft / synapse / cell surface / protein-containing complex / zinc ion binding / nucleus / plasma membrane / cytosol
Similarity search - Function
Dystrophin/utrophin / EF-hand domain, type 1 / EF-hand domain, type 2 / EF hand / EF-hand / Spectrin repeat / Spectrin repeat / Spectrin/alpha-actinin / Actinin-type actin-binding domain signature 1. / Actinin-type actin-binding domain signature 2. ...Dystrophin/utrophin / EF-hand domain, type 1 / EF-hand domain, type 2 / EF hand / EF-hand / Spectrin repeat / Spectrin repeat / Spectrin/alpha-actinin / Actinin-type actin-binding domain signature 1. / Actinin-type actin-binding domain signature 2. / Spectrin repeats / Actinin-type actin-binding domain, conserved site / Calponin homology domain / Zinc finger ZZ-type signature. / Zinc-binding domain, present in Dystrophin, CREB-binding protein. / Calponin homology (CH) domain / Zinc finger, ZZ type / Zinc finger, ZZ-type / Zinc finger, ZZ-type superfamily / Zinc finger ZZ-type profile. / Calponin homology domain / CH domain superfamily / Calponin homology (CH) domain profile. / WW domain / WW/rsp5/WWP domain signature. / WW domain superfamily / WW/rsp5/WWP domain profile. / Domain with 2 conserved Trp (W) residues / WW domain / EF-hand domain pair
Similarity search - Domain/homology
Contact author
  • Raphael Dos Santos Morais (University of Lorraine)

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Structure visualization

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Models

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Sample

SampleName: R4-15 human dystrophin fragment
BufferName: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2% / pH: 7.5
Entity #1556Type: protein / Description: Human dystrophin central domain R4-15 fragment / Formula weight: 150.486 / Num. of mol.: 1 / References: UniProt: P11532
Sequence: MSYYHHHHHH DYDIPTTENL YFOGAMGRSL VPRGSSEIRK RLDVDITELH SWITRSEAVL QSPEFAIFRK EGNFSDLKEK VNAIEREKAE KFRKLQDASR SAQALVEQMV NEGVNADSIK QASEQLNSRW IEFCQLLSER LNWLEYQNNI IAFYNQLQQL EQMTTTAENW ...Sequence:
MSYYHHHHHH DYDIPTTENL YFOGAMGRSL VPRGSSEIRK RLDVDITELH SWITRSEAVL QSPEFAIFRK EGNFSDLKEK VNAIEREKAE KFRKLQDASR SAQALVEQMV NEGVNADSIK QASEQLNSRW IEFCQLLSER LNWLEYQNNI IAFYNQLQQL EQMTTTAENW LKIQPTTPSE PTAIKSQLKI CKDEVNRLSG LQPQIERLKI QSIALKEKGQ GPMFLDADFV AFTNHFKQVF SDVQAREKEL QTIFDTLPPM RYQETMSAIR TWVQQSETKL SIPQLSVTDY EIMEQRLGEL QALQSSLQEQ QSGLYYLSTT VKEMSKKAPS EISRKYQSEF EEIEGRWKKL SSQLVEHCQK LEEQMNKLRK IQNHIQTLKK WMAEVDVFLK EEWPALGDSE ILKKQLKQCR LLVSDIQTIQ PSLNSVNEGG QKIKNEAEPE FASRLETELK ELNTQWDHMC QQVYARKEAL KGGLEKTVSL QKDLSEMHEW MTQAEEEYLE RDFEYKTPDE LQKAVEEMKR AKEEAQQKEA KVKLLTESVN SVIAQAPPVA QEALKKELET LTTNYQWLCT RLNGKCKTLE EVWACWHELL SYLEKANKWL NEVEFKLKTT ENIPGGAEEI SEVLDSLENL MRHSEDNPNQ IRILAQTLTD GGVMDELINE ELETFNSRWR ELHEEAVRRQ KLLEQSIQSA QETEKSLHLI QESLTFIDKQ LAAYIADKVD AAQMPQEAQK IQSDLTSHEI SLEEMKKHNQ GKEAAQRVLS QIDVAQKKLQ DVSMKFRLFQ KPANFELRLQ ESKMILDEVK MHLPALETKS VEQEVVQSQL NHCVNLYKSL SEVKSEVEMV IKTGRQIVQK KQTENPKELD ERVTALKLHY NELGAKVTER KQQLEKCLKL SRKMRKEMNV LTEWLAATDM ELTKRSAVEG MPSNLDSEVA WGKATQKEIE KQKVHLKSIT EVGEALKTVL GKKETLVEDK LSLLNSNWIA VTSRAEEWLN LLLEYQKHME TFDQNVDHIT KWIIQADTLL DESEKKKPQQ KEDVLKRLKA ELNDIRPKVD STRDQAANLM ANRGDHCRKL VEPQISELNH RFAAISHRIK TGKASIPLKE LEQFNSDIQK LLEPLEAEIQ QGVNLKEEDF NKDMNEDNEG TVKELLQRGD NLQQRITDER KREEIKIKQQ LLQTKHNALK DLRSQRRKKA LEISHQWYQY KRQADDLLKC LDDIEKKLAS LPEPRDERKI KEIDRELQKK KEELNAVRRQ AEGLSEDGAA MAVEPTQIQL SKRWREIESK FAQFRRLNFA Q

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Experimental information

BeamInstrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 2 mm
DetectorName: AVIEX PCCD170170 / Type: CCD
Scan
Title: R4-15 human dystrophin fragment / Measurement date: May 26, 2016 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 21 / Unit: 1/A /
MinMax
Q0.0051 0.5496
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 444 /
MinMax
Q0.008115 0.2327
P(R) point6 449
R0 475
Result
Experimental MW: 150 kDa / D max: 47.5 / Type of curve: sec
Comments: SEC-SAXS was performed at 20°C using the following parameters: Column: BioSEC5-500Å (4.6 mm id * 300 mm); Flow rate: 0.3 mL/min; Sample injection concentration: 4.4 mg/mL; Injection ...Comments: SEC-SAXS was performed at 20°C using the following parameters: Column: BioSEC5-500Å (4.6 mm id * 300 mm); Flow rate: 0.3 mL/min; Sample injection concentration: 4.4 mg/mL; Injection volume: 50μL. The data were collected through the SEC peak of the protein as a series of 21 x 1 second exposures. The experimental molecular weight was determined from SEC-MALS (150 kDa).
P(R)Guinier
Forward scattering, I01.742 1.53
Radius of gyration, Rg12.57 nm9.69 nm

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