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Yorodumi- SASDFY3: Semi-synthetic nucleosome core particle (NCP) in complex with lys... -
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-Basic information
Entry | Database: SASBDB / ID: SASDFY3 |
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Sample | Semi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205)
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Biological species | Homo sapiens (human) Xenopus laevis (African clawed frog) synthetic construct (others) |
-Structure visualization
-Downloads & links
-Data source
SASBDB page | SASDFY3 |
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-Models
-Sample
Sample | Name: Semi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205) Specimen concentration: 1.8 mg/ml / Entity id: 1516 / 1519 / 1520 / 1521 / 1522 / 1523 / 1524 |
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Buffer | Name: 15 mM HEPES, 200 mM NaCl / pH: 7.3 |
Entity #1516 | Name: LSD2 / Type: protein / Description: Lysyne-specific Demethylase LSD2 / Formula weight: 88.832 / Num. of mol.: 1 / Source: Homo sapiens Sequence: KKKATETTDE DEDGGSEKKY RKCEKAGCTA TCPVCFASAS ERCAKNGYTS RWYHLSCGEH FCNECFDHYY RSHKDGYDKY TTWKKIWTSN GKTEPSPKAF MADQQLPYWV QCTKPECRKW RQLTKEIQLT PQIAKTYRCG MKPNTAIKPE TSDHCSLPED LRVLEVSNHW ...Sequence: KKKATETTDE DEDGGSEKKY RKCEKAGCTA TCPVCFASAS ERCAKNGYTS RWYHLSCGEH FCNECFDHYY RSHKDGYDKY TTWKKIWTSN GKTEPSPKAF MADQQLPYWV QCTKPECRKW RQLTKEIQLT PQIAKTYRCG MKPNTAIKPE TSDHCSLPED LRVLEVSNHW WYSMLILPPL LKDSVAAPLL SAYYPDCVGM SPSCTSTNRA AATGNASPGK LEHSKAALSV HVPGMNRYFQ PFYQPNECGK ALCVRPDVME LDELYEFPEY SRDPTMYLAL RNLILALWYT NCKEALTPQK CIPHIIVRGL VRIRCVQEVE RILYFMTRKG LINTGVLSVG ADQYLLPKDY HNKSVIIIGA GPAGLAAARQ LHNFGIKVTV LEAKDRIGGR VWDDKSFKGV TVGRGAQIVN GCINNPVALM CEQLGISMHK FGERCDLIQE GGRITDPTID KRMDFHFNAL LDVVSEWRKD KTQLQDVPLG EKIEEIYKAF IKESGIQFSE LEGQVLQFHL SNLEYACGSN LHQVSARSWD HNEFFAQFAG DHTLLTPGYS VIIEKLAEGL DIQLKSPVQC IDYSGDEVQV TTTDGTGYSA QKVLVTVPLA LLQKGAIQFN PPLSEKKMKA INSLGAGIIE KIALQFPYRF WDSKVQGADF FGHVPPSASK RGLFAVFYDM DPQKKHSVLM SVIAGEAVAS VRTLDDKQVL QQCMATLREL FKEQEVPDPT KYFVTRWSTD PWIQMAYSFV KTGGSGEAYD IIAEDIQGTV FFAGEATNRH FPQTVTGAYL SGVREASKIA AF |
Entity #1519 | Name: NPAC delta205 / Type: protein / Description: NPAC linker+DH (delta-205) / Formula weight: 37.438 / Num. of mol.: 4 / Source: Homo sapiens Sequence: TASEPVKDAD PHFHHFLLSQ TEKPAVCYQA ITKKLKICEE ETGSTSIQAA DSTAVNGSIT PTDKKIGFLG LGLMGSGIVS NLLKMGHTVT VWNRTAEKCD LFIQEGARLG RTPAEVVSTC DITFACVSDP KAAKDLVLGP SGVLQGIRPG KCYVDMSTVD ADTVTELAQV ...Sequence: TASEPVKDAD PHFHHFLLSQ TEKPAVCYQA ITKKLKICEE ETGSTSIQAA DSTAVNGSIT PTDKKIGFLG LGLMGSGIVS NLLKMGHTVT VWNRTAEKCD LFIQEGARLG RTPAEVVSTC DITFACVSDP KAAKDLVLGP SGVLQGIRPG KCYVDMSTVD ADTVTELAQV IVSRGGRFLE APVSGNQQLS NDGMLVILAA GDRGLYEDCS SCFQAMGKTS FFLGEVGNAA KMMLIVNMVQ GSFMATIAEG LTLAQVTGQS QQTLLDILNQ GQLASIFLDQ KCQNILQGNF KPDFYLKYIQ KDLRLAIALG DAVNHPTPMA AAANEVYKRA KALDQSDNDM SAVYRAYIH |
Entity #1520 | Name: H3 / Type: protein / Description: Histone H3 / Formula weight: 15.271 / Num. of mol.: 2 / Source: Xenopus laevis Sequence: ARTKQTARKS TGGKAPRKQL ATKAARKSAP ATGGVKKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSSAVM ALQEASEAYL VALFEDTNLC AIHAKRVTIM PKDIQLARRI RGERA |
Entity #1521 | Name: H4 / Type: protein / Description: Histone H4 / Formula weight: 11.236 / Num. of mol.: 2 / Source: Xenopus laevis Sequence: SGRGKGGKGL GKGGAKRHRK VLRDNIQGIT KPAIRRLARR GGVKRISGLI YEETRGVLKV FLENVIRDAV TYTEHAKRKT VTAMDVVYAL KRQGRTLYGF GG |
Entity #1522 | Name: H2a / Type: protein / Description: Histone H2a / Formula weight: 13.95 / Num. of mol.: 2 / Source: Xenopus laevis Sequence: SGRGKQGGKT RAKAKTRSSR AGLQFPVGRV HRLLRKGNYA ERVGAGAPVY LAAVLEYLTA EILELAGNAA RDNKKTRIIP RHLQLAVRND EELNKLLGRV TIAQGGVLPN IQSVLLPKKT ESSKSAKSK |
Entity #1523 | Name: H2b / Type: protein / Description: Histone H2b / Formula weight: 13.494 / Num. of mol.: 2 / Source: Xenopus laevis Sequence: AKSAPAPKKG SKKAVTKTQK KDGKKRRKTR KESYAIYVYK VLKQVHPDTG ISSKAMSIMN SFVNDVFERI AGEASRLAHY NKRSTITSRE IQTAVRLLLP GELAKHAVSE GTKAVTKYTS AK |
Entity #1524 | Name: 601-147bp / Type: DNA / Description: 147bp 601 Widom sequence / Formula weight: 45.202 / Num. of mol.: 1 / Source: synthetic construct Sequence: ATCGAGAATC CCGGTGCCGA GGCCGCTCAA TTGGTCGTAG ACAGCTCTAG CACCGCTTAA ACGCACGTAC GCGCTGTCCC CCGCGTTTTA ACCGCCAAGG GGATTACTCC CTAGTCTCCA GGCACGTGTC AGATATATAC ATCCGAT |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 2.87 mm | ||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
Scan | Title: Semi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205) Measurement date: Dec 11, 2017 / Storage temperature: 4 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 70 / Unit: 1/nm /
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Result | Type of curve: sec /
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