SASDFX8: Apolipoprotein E2 (ApoE2) bound to 37 µg/mL heparin

Basic information

Entry

Database: SASBDB / ID: SASDFX8

Sample

Apolipoprotein E2 (ApoE2) bound to 37 µg/mL heparin

• Apolipoprotein E2 (protein), ApoE2, Homo sapiens
• Heparin (other)

Function / homology

Function:

lipid transport involved in lipid storage / intermediate-density lipoprotein particle clearance / positive regulation of lipid transport across blood-brain barrier / regulation of cellular response to very-low-density lipoprotein particle stimulus / metal chelating activity / triglyceride-rich lipoprotein particle clearance / discoidal high-density lipoprotein particle / lipoprotein particle / negative regulation of triglyceride metabolic process / negative regulation of cholesterol biosynthetic process / regulation of amyloid-beta clearance / maintenance of location in cell / positive regulation of lipoprotein transport / Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors / chylomicron remnant clearance / chylomicron remnant / intermediate-density lipoprotein particle / acylglycerol homeostasis / NMDA glutamate receptor clustering / very-low-density lipoprotein particle remodeling / phosphatidylcholine-sterol O-acyltransferase activator activity / response to caloric restriction / Chylomicron clearance / positive regulation of phospholipid efflux / very-low-density lipoprotein particle clearance / Chylomicron remodeling / lipid transporter activity / cellular response to lipoprotein particle stimulus / positive regulation of low-density lipoprotein particle receptor catabolic process / regulation of amyloid fibril formation / Chylomicron assembly / high-density lipoprotein particle clearance / chylomicron / phospholipid efflux / regulation of protein metabolic process / very-low-density lipoprotein particle receptor binding / high-density lipoprotein particle remodeling / lipoprotein catabolic process / AMPA glutamate receptor clustering / melanosome organization / positive regulation of cholesterol metabolic process / multivesicular body, internal vesicle / regulation of behavioral fear response / reverse cholesterol transport / positive regulation of amyloid-beta clearance / host-mediated activation of viral process / high-density lipoprotein particle assembly / low-density lipoprotein particle / lipoprotein biosynthetic process / cholesterol transfer activity / high-density lipoprotein particle / protein import / very-low-density lipoprotein particle / cholesterol catabolic process / low-density lipoprotein particle remodeling / heparan sulfate proteoglycan binding / amyloid precursor protein metabolic process / negative regulation of amyloid fibril formation / regulation of amyloid precursor protein catabolic process / positive regulation of membrane protein ectodomain proteolysis / regulation of Cdc42 protein signal transduction / synaptic transmission, cholinergic / HDL remodeling / negative regulation of endothelial cell migration / cholesterol efflux / regulation of cholesterol metabolic process / negative regulation of protein metabolic process / regulation of axon extension / triglyceride homeostasis / artery morphogenesis / Scavenging by Class A Receptors / triglyceride metabolic process / low-density lipoprotein particle receptor binding / positive regulation of amyloid fibril formation / regulation of innate immune response / virion assembly / positive regulation of dendritic spine development / negative regulation of endothelial cell proliferation / negative regulation of amyloid-beta formation / locomotory exploration behavior / antioxidant activity / response to dietary excess / lipoprotein particle binding / negative regulation of MAP kinase activity / negative regulation of blood vessel endothelial cell migration / positive regulation of endocytosis / negative regulation of long-term synaptic potentiation / negative regulation of platelet activation / positive regulation of dendritic spine maintenance / negative regulation of blood coagulation / positive regulation of cholesterol efflux / regulation of neuronal synaptic plasticity / long-term memory / negative regulation of protein secretion / fatty acid homeostasis / long-chain fatty acid transport / synaptic cleft / regulation of protein-containing complex assembly / intracellular transport / positive regulation of lipid biosynthetic process

Domain/homology:

Apolipoprotein A/E / : / Apolipoprotein A1/A4/E domain

Component:

Apolipoprotein E

• Biological species: Homo sapiens (human)

Contact author

• Lucas Kraft (University of Sussex, Brighton, UK)

Models

Sample

• Sample: Name: Apolipoprotein E2 (ApoE2) bound to 37 µg/mL heparin / Specimen concentration: 4 mg/ml / Entity id: 1086 / 1633
• Buffer: Name: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP / pH: 8

Entity #1086

Name: ApoE2 / Type: protein / Description: Apolipoprotein E2 / Formula weight: 34.606 / Num. of mol.: 4 / Source: Homo sapiens / References: UniProt: P02649
Sequence:

GPHMKVEQAV ETEPEPELRQ QTEWQSGQRW ELALGRFWDY LRWVQTLSEQ VQEELLSSQV TQELRALMDE TMKELKAYKS ELEEQLTPVA EETRARLSKE LQAAQARLGA DMEDVCGRLV QYRGEVQAML GQSTEELRVR LASHLRKLRK RLLRDADDLQ KCLAVYQAGA REGAERGLSA IRERLGPLVE QGRVRAATVG SLAGQPLQER AQAWGERLRA RMEEMGSRTR DRLDEVKEQV AEVRAKLEEQ AQQIRLQAEA FQARLKSWFE PLVEDMQRQW AGLVEKVQAA VGTSAAPVPS DNH

Entity #1633

Type: other / Description: Heparin / Formula weight: 15 / Num. of mol.: 1
Sequence:

(C12H19NO20S3)n

Experimental information

• Beam: Instrument name: Diamond Light Source B21 / City: Didcot / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.036 mm
• Detector: Name: Pilatus 2M

Scan

Title: Apolipoprotein E2 (ApoE2) bound to 37 µg/mL heparin / Measurement date: May 3, 2018 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 28 / Unit: 1/A /

	Min	Max
Q	0.003	0.37

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 1008 /

	Min	Max
Q	0.010567	0.234684
P(R) point	1	1008
R	0	234

Result

Type of curve: single_conc
Comments: ApoE2 + 37 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.

	Experimental	Porod
MW	206 kDa	-
Volume	-	584 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.5559	0.553	0.0049
Radius of gyration, Rg	6.75 nm	6.527 nm	0.081

	Min	Max
D	-	23.4
Guinier point	35	75