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Yorodumi- SASDFX6: DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80... -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDFX6 |
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Sample | DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:4)
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Function / homology | Function and homology information HU-DNA complex / DnaA-HU complex / bacterial nucleoid packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding ...HU-DNA complex / DnaA-HU complex / bacterial nucleoid packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding / membrane / identical protein binding / cytosol Similarity search - Function |
Biological species | Escherichia coli (E. coli) Escherichia coli K-12 (bacteria) |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Data source
SASBDB page | SASDFX6 |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Models
Model #3027 | Type: atomic / Chi-square value: 1.7613483923 |
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-Sample
Sample | Name: DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:4) Entity id: 1622 / 1623 / 1658 |
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Buffer | Name: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF / pH: 7.5 |
Entity #1622 | Type: DNA / Description: 80bp_DNA Forward / Formula weight: 24.849 / Num. of mol.: 1 / Source: Escherichia coli Sequence: AATGAGGTAA CAACGAAAGC AGATGATAGC TGCTTATCAA TTTGTTGCAA ACAAGTAGCC GCGCCCAATG AGGTAACAAT |
Entity #1623 | Type: DNA / Description: 80bp_DNA Reverse / Formula weight: 24.612 / Num. of mol.: 1 / Source: Escherichia coli K-12 Sequence: TTACTCCATT GTTGCTTTCG TCTACTATCG ACGAATAGTT AAACAACGTT TGTTCATCGG CGCGGGTTAC TCCATTGTTA |
Entity #1658 | Type: protein Description: DNA-binding protein HU-alpha, E38K/V42L double mutant Formula weight: 9.548 / Num. of mol.: 4 / Source: Escherichia coli K-12 / References: UniProt: P0ACF0 Sequence: MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITESLKKGD ALQLVGFGTF KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK |
-Experimental information
Beam | Instrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS) City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 1.5 mm | |||||||||||||||||||||
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Detector | Name: MAR 165 CCD | |||||||||||||||||||||
Scan | Title: DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:4) Measurement date: Apr 23, 2015 / Cell temperature: 10 °C / Exposure time: 0.1 sec. / Number of frames: 32 / Unit: 1/A /
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Distance distribution function P(R) | Sofotware P(R): GNOM 4.6 / Number of points: 499 /
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Result | D max: 25.65 / Type of curve: single_conc /
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