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- SASDFV6: DNA-binding protein HU-alpha, E38K/V42L double mutant -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDFV6
SampleDNA-binding protein HU-alpha, E38K/V42L double mutant
  • DNA-binding protein HU-alpha, E38K/V42L double mutant (protein), Escherichia coli K-12
Function / homology
Function and homology information


HU-DNA complex / DnaA-HU complex / bacterial nucleoid DNA packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding ...HU-DNA complex / DnaA-HU complex / bacterial nucleoid DNA packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding / identical protein binding / membrane / cytosol
Similarity search - Function
Histone-like DNA-binding protein, conserved site / Bacterial histone-like DNA-binding proteins signature. / Histone-like DNA-binding protein / Bacterial DNA-binding protein / bacterial (prokaryotic) histone like domain / Integration host factor (IHF)-like DNA-binding domain superfamily
Similarity search - Domain/homology
DNA-binding protein HU-alpha
Similarity search - Component
Biological speciesEscherichia coli K-12 (bacteria)
Contact author
  • Soumya G Remesh (Lawrence Berkeley National laboratory)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3004
Type: atomic / Chi-square value: 2.34225022748301
Model #3005
Type: atomic / Chi-square value: 2.34225022748301

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Sample

SampleName: DNA-binding protein HU-alpha, E38K/V42L double mutant
BufferName: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF / pH: 7.5
Entity #1649Type: protein
Description: DNA-binding protein HU-alpha, E38K/V42L double mutant
Formula weight: 9.548 / Num. of mol.: 10 / Source: Escherichia coli K-12 / References: UniProt: P0ACF0
Sequence:
MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITESLKKGD ALQLVGFGTF KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK

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Experimental information

BeamInstrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS)
City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 1.5 mm
DetectorName: MAR 165 CCD
Scan
Title: DNA-binding protein HU-alpha, E38K/V42L double mutant
Measurement date: Apr 23, 2015 / Cell temperature: 10 °C / Exposure time: 0.1 sec. / Number of frames: 32 / Unit: 1/A /
MinMax
Q0.0143 0.3214
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 412 /
MinMax
Q0.014899 0.264819
P(R) point1 412
R0 105.4
Result
Type of curve: single_conc /
ExperimentalPorod
MW60 kDa-
Volume-53 nm3

P(R)GuinierGuinier error
Forward scattering, I059.14 59.96 0.68
Radius of gyration, Rg3.06 nm3.02 nm0.104

MinMax
D-10.54
Guinier point2 47

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