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- SASDFT6: DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (rat... -

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Basic information

Entry
Database: SASBDB / ID: SASDFT6
SampleDNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:5)
  • 80bp_DNA Forward (DNA), Escherichia coli
  • 80bp_DNA Reverse (DNA), Escherichia coli K-12
  • DNA-binding protein HU-alpha, E34K (protein), Escherichia coli K-12
Function / homology
Function and homology information


HU-DNA complex / DnaA-HU complex / bacterial nucleoid packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding ...HU-DNA complex / DnaA-HU complex / bacterial nucleoid packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding / identical protein binding / membrane / cytosol
Similarity search - Function
Histone-like DNA-binding protein, conserved site / Bacterial histone-like DNA-binding proteins signature. / Histone-like DNA-binding protein / Bacterial DNA-binding protein / bacterial (prokaryotic) histone like domain / Integration host factor (IHF)-like DNA-binding domain superfamily
Similarity search - Domain/homology
DNA-binding protein HU-alpha
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
Escherichia coli K-12 (bacteria)
Contact author
  • Soumya G Remesh (Lawrence Berkeley National laboratory)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3019
Type: atomic
Model #3020
Type: atomic

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Sample

SampleName: DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:5)
Entity id: 1622 / 1623 / 1656
BufferName: 20mM HEPES, 100mM NaCl / pH: 7.5
Entity #1622Type: DNA / Description: 80bp_DNA Forward / Formula weight: 24.849 / Num. of mol.: 1 / Source: Escherichia coli
Sequence:
AATGAGGTAA CAACGAAAGC AGATGATAGC TGCTTATCAA TTTGTTGCAA ACAAGTAGCC GCGCCCAATG AGGTAACAAT
Entity #1623Type: DNA / Description: 80bp_DNA Reverse / Formula weight: 24.612 / Num. of mol.: 1 / Source: Escherichia coli K-12
Sequence:
TTACTCCATT GTTGCTTTCG TCTACTATCG ACGAATAGTT AAACAACGTT TGTTCATCGG CGCGGGTTAC TCCATTGTTA
Entity #1656Type: protein / Description: DNA-binding protein HU-alpha, E34K / Formula weight: 9.534 / Source: Escherichia coli K-12 / References: UniProt: P0ACF0
Sequence:
MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITKSLKEGD AVQLVGFGTF KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK

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Experimental information

BeamInstrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS)
City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 1.5 mm
DetectorName: Pilatus3 X 2M / Pixsize x: 172 mm
Scan
Title: DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:5)
Measurement date: Jul 8, 2016 / Cell temperature: 10 °C / Exposure time: 3 sec. / Number of frames: 300 / Unit: 1/A /
MinMax
Q0.0114 0.3236
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 393 /
MinMax
Q0.01533 0.233833
P(R) point1 393
R0 270
Result
D max: 27 / Type of curve: single_conc /
ExperimentalPorod
MW360 kDa-
Volume-474 nm3

P(R)GuinierGuinier error
Forward scattering, I065.42 63.82 0.64
Radius of gyration, Rg7.82 nm7.188 nm0.57

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