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- SASDFR7: Rabbit muscle fructose-bisphosphate aldolase A, K229M mutant -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDFR7
SampleRabbit muscle fructose-bisphosphate aldolase A, K229M mutant
  • Fructose-bisphosphate aldolase A (protein), Aldolase, Oryctolagus cuniculus
Function / homology
Function and homology information


negative regulation of Arp2/3 complex-mediated actin nucleation / fructose-bisphosphate aldolase / fructose-bisphosphate aldolase activity / M band / I band / glycolytic process / protein homotetramerization / positive regulation of cell migration
Similarity search - Function
Fructose-bisphosphate aldolase class-I active site / Fructose-bisphosphate aldolase class-I active site. / Fructose-bisphosphate aldolase, class-I / Fructose-bisphosphate aldolase class-I / Aldolase-type TIM barrel
Similarity search - Domain/homology
Fructose-bisphosphate aldolase A
Similarity search - Component
Biological speciesOryctolagus cuniculus (rabbit)
Contact author
  • Normand Cyr (Département de biochimie, Université de Montréal, Montréal, Canada)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3194
Type: atomic / Symmetry: P222 / Chi-square value: 1.659 / P-value: 0.000021
Model #3193
Type: dummy / Radius of dummy atoms: 3.50 A / Symmetry: P1 / Chi-square value: 1.178 / P-value: 0.008512

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Sample

SampleName: Rabbit muscle fructose-bisphosphate aldolase A, K229M mutant
Specimen concentration: 8.66 mg/ml
BufferName: 20 mM HEPES / pH: 7
Entity #1735Name: Aldolase / Type: protein / Description: Fructose-bisphosphate aldolase A / Formula weight: 39.214 / Num. of mol.: 4 / Source: Oryctolagus cuniculus / References: UniProt: P00883
Sequence: PHSHPALTPE QKKELSDIAH RIVAPGKGIL AADESTGSIA KRLQSIGTEN TEENRRFYRQ LLLTADDRVN PCIGGVILFH ETLYQKADDG RPFPQVIKSK GGVVGIKVDK GVVPLAGTNG ETTTQGLDGL SERCAQYKKD GADFAKWRCV LKIGEHTPSA LAIMENANVL ...Sequence:
PHSHPALTPE QKKELSDIAH RIVAPGKGIL AADESTGSIA KRLQSIGTEN TEENRRFYRQ LLLTADDRVN PCIGGVILFH ETLYQKADDG RPFPQVIKSK GGVVGIKVDK GVVPLAGTNG ETTTQGLDGL SERCAQYKKD GADFAKWRCV LKIGEHTPSA LAIMENANVL ARYASICQQN GIVPIVEPEI LPDGDHDLKR CQYVTEKVLA AVYKALSDHH IYLEGTLLMP NMVTPGHACT QKYSHEEIAM ATVTALRRTV PPAVTGVTFL SGGQSEEEAS INLNAINKCP LLKPWALTFS YGRALQASAL KAWGGKKENL KAAQEEYVKR ALANSLACQG KYTPSGQAGA AASESLFISN HAY

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Experimental information

BeamInstrument name: Université de Montréal Xenocs BioXolver L with MetalJet
City: Montréal / 国: Canada / Type of source: X-ray in house / Wavelength: 1.348 Å / Dist. spec. to detc.: 600 mm
DetectorName: Pilatus3 R 300K / Pixsize x: 172 mm
Scan
Title: Rabbit muscle fructose-bisphosphate aldolase A, K229M mutant
Measurement date: Jun 14, 2019 / Storage temperature: 4 °C / Cell temperature: 20 °C / Exposure time: 60 sec. / Number of frames: 10 / Unit: 1/A /
MinMax
Q0.0107 0.3999
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 429 /
MinMax
Q0.0106922 0.579347
P(R) point1 429
R0 118
Result
Type of curve: single_conc
ExperimentalStandardPorod
MW157.3 kDa160 kDa133 kDa
Volume--213 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.1303 0.0002174 0.1302 0.0002708
Radius of gyration, Rg3.6 nm0.007 3.602 nm0.011

MinMax
D-11.8
Guinier point1 20

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