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- SASDFQ6: DNA-binding protein HU-alpha, E34K -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDFQ6
SampleDNA-binding protein HU-alpha, E34K
  • DNA-binding protein HU-alpha, E34K (protein), Escherichia coli K-12
Function / homology
Function and homology information


HU-DNA complex / DnaA-HU complex / bacterial nucleoid packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding ...HU-DNA complex / DnaA-HU complex / bacterial nucleoid packaging / chromosome condensation / DNA replication initiation / structural constituent of chromatin / DNA repair / DNA-templated transcription / DNA damage response / DNA binding / identical protein binding / membrane / cytosol
Similarity search - Function
Histone-like DNA-binding protein, conserved site / Bacterial histone-like DNA-binding proteins signature. / Histone-like DNA-binding protein / Bacterial DNA-binding protein / bacterial (prokaryotic) histone like domain / Integration host factor (IHF)-like DNA-binding domain superfamily
Similarity search - Domain/homology
DNA-binding protein HU-alpha
Similarity search - Component
Biological speciesEscherichia coli K-12 (bacteria)
Contact author
  • Soumya G Remesh (Lawrence Berkeley National laboratory)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2976
Type: atomic / Chi-square value: 4.03449454684557

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Sample

SampleName: DNA-binding protein HU-alpha, E34K
BufferName: 10 mM Bis-Tris, 100 mM NaCl / pH: 7.5
Entity #1632Type: protein / Description: DNA-binding protein HU-alpha, E34K / Formula weight: 9.534 / Num. of mol.: 2 / Source: Escherichia coli K-12 / References: UniProt: P0ACF0
Sequence:
MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITKSLKEGD AVQLVGFGTF KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK

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Experimental information

BeamInstrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS)
City: Berkeley, CA / : USA / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 1.5 mm
DetectorName: Pilatus3 X 2M / Pixsize x: 172 mm
Scan
Title: DNA-binding protein HU-alpha, E34K / Measurement date: Jun 1, 2018 / Cell temperature: 10 °C / Exposure time: 3 sec. / Number of frames: 300 / Unit: 1/A /
MinMax
Q0.0114 0.3236
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 428 /
MinMax
Q0.023692 0.261699
P(R) point1 428
R0 66.67
Result
Type of curve: single_conc /
ExperimentalPorod
MW20 kDa-
Volume-36 nm3

P(R)GuinierGuinier error
Forward scattering, I0442.6 449.42 1.71
Radius of gyration, Rg2.16 nm2.18 nm0.213

MinMax
D-6.67
Guinier point23 87

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